bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.)
SUMMARY Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by...
Published in: | Molecular Reproduction and Development |
---|---|
Main Authors: | , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2016
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/mrd.22755 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.22755 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22755 |
id |
crwiley:10.1002/mrd.22755 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/mrd.22755 2024-06-23T07:51:15+00:00 bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.) Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Juanchich, Amélie Wargelius, Anna U.S. Nuclear Regulatory Commission 2016 http://dx.doi.org/10.1002/mrd.22755 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.22755 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22755 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Reproduction and Development volume 84, issue 1, page 76-87 ISSN 1040-452X 1098-2795 journal-article 2016 crwiley https://doi.org/10.1002/mrd.22755 2024-05-31T08:13:32Z SUMMARY Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad‐specific genes using available adult multi‐tissue transcriptomes. We excluded genes with expression in tissues other than gonads based on quantity of reads, and then a subset of genes was selected for verification in a multi‐tissue PCR screen. Four gonad‐specific genes ( bmp15l , figla , smc1bl , and larp6l ) were chosen for further characterization, namely: germ cell specificity, investigated by comparing mRNA abundance in wild‐type and germ cell‐free gonads by quantitative real‐time PCR, and cellular location, visualized by in situ hybridization. All four genes were expressed in both testis and ovary, and preferentially within the germ cells of both sexes. These genes may be essential players in salmon germ cell development, and could be important for future studies aiming to understand and control reproduction. Mol. Reprod. Dev. 84: 76–87, 2017. © 2016 Wiley Periodicals, Inc . Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Molecular Reproduction and Development 84 1 76 87 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
SUMMARY Atlantic salmon is a valuable commercial aquaculture species that would benefit economically and environmentally by controlling precocious puberty and preventing escapees from reproducing with wild populations. One solution to both these challenges is the production of sterile individuals by inhibiting the formation of germ cells, but achieving this requires more information on the specific factors that control germ cell formation. Here, we identified and characterized novel factors that are preferentially expressed in Atlantic salmon germ cells by screening for gonad‐specific genes using available adult multi‐tissue transcriptomes. We excluded genes with expression in tissues other than gonads based on quantity of reads, and then a subset of genes was selected for verification in a multi‐tissue PCR screen. Four gonad‐specific genes ( bmp15l , figla , smc1bl , and larp6l ) were chosen for further characterization, namely: germ cell specificity, investigated by comparing mRNA abundance in wild‐type and germ cell‐free gonads by quantitative real‐time PCR, and cellular location, visualized by in situ hybridization. All four genes were expressed in both testis and ovary, and preferentially within the germ cells of both sexes. These genes may be essential players in salmon germ cell development, and could be important for future studies aiming to understand and control reproduction. Mol. Reprod. Dev. 84: 76–87, 2017. © 2016 Wiley Periodicals, Inc . |
author2 |
U.S. Nuclear Regulatory Commission |
format |
Article in Journal/Newspaper |
author |
Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Juanchich, Amélie Wargelius, Anna |
spellingShingle |
Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Juanchich, Amélie Wargelius, Anna bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.) |
author_facet |
Kleppe, Lene Edvardsen, Rolf Brudvik Furmanek, Tomasz Andersson, Eva Juanchich, Amélie Wargelius, Anna |
author_sort |
Kleppe, Lene |
title |
bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.) |
title_short |
bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.) |
title_full |
bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.) |
title_fullStr |
bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.) |
title_full_unstemmed |
bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in Atlantic salmon ( Salmo salar L.) |
title_sort |
bmp15l, figla, smc1bl, and larp6l are preferentially expressed in germ cells in atlantic salmon ( salmo salar l.) |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1002/mrd.22755 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.22755 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22755 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Molecular Reproduction and Development volume 84, issue 1, page 76-87 ISSN 1040-452X 1098-2795 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/mrd.22755 |
container_title |
Molecular Reproduction and Development |
container_volume |
84 |
container_issue |
1 |
container_start_page |
76 |
op_container_end_page |
87 |
_version_ |
1802642285705297920 |