Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.)

Abstract The factors of the Sox9‐Amh‐Cyp19a1 cascade play a crucial role in the complex process of sex differentiation in mammals. The involvement of Sox9 and Cyp19a1 paralogs and the single Amh ortholog in sex differentiation and development of the gonads and the brain in Atlantic cod was examined...

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Published in:Molecular Reproduction and Development
Main Authors: Johnsen, Hanne, Tveiten, Helge, Torgersen, Jacob S., Andersen, Øivind
Other Authors: The Research Council of Norway
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2013
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Online Access:http://dx.doi.org/10.1002/mrd.22170
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spelling crwiley:10.1002/mrd.22170 2024-09-15T17:55:28+00:00 Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.) Johnsen, Hanne Tveiten, Helge Torgersen, Jacob S. Andersen, Øivind The Research Council of Norway 2013 http://dx.doi.org/10.1002/mrd.22170 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.22170 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22170 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Reproduction and Development volume 80, issue 5, page 358-370 ISSN 1040-452X 1098-2795 journal-article 2013 crwiley https://doi.org/10.1002/mrd.22170 2024-07-23T04:16:12Z Abstract The factors of the Sox9‐Amh‐Cyp19a1 cascade play a crucial role in the complex process of sex differentiation in mammals. The involvement of Sox9 and Cyp19a1 paralogs and the single Amh ortholog in sex differentiation and development of the gonads and the brain in Atlantic cod was examined by analyzing bimodal and sex‐dimorphic gene expression patterns, respectively, during early stages and in maturing males and females. Expression of sox9a and sox9b were initiated at blastulation, and both paralogs were expressed in chondrogenic tissue in the hatched larvae. The male‐specific expression of sox9a in the adult gonads supports a conserved role in testis function, while sox9b was expressed in the maturing testes and ovaries at similar levels. Amh was expressed at low, but variable, levels from late gastrulation prior to the onset of cyp19a1a and cyp19a1b expression. Male‐biased amh expression was found in the maturing gonads, but the increased ovarian levels during maturation suggest a role also in females. The larval expression of cyp19a1a and cyp19a1b increased at the expected time of sex differentiation, but showed large individual variation. The ovarian expression of cyp19a1a and amh increased concomitant with increased plasma estradiol levels during vitellogenesis. The testis‐specific cyp19a1b expression supports the importance of estrogen in the spermatogenesis, while abundant expression in the male and female brain is probably related to the continuous neurogenesis in fish. These divergent and sex‐dimorphic expression patterns of the cod sox9 and cyp19a1 paralogs demonstrate the complexity of the genetic network regulating sexual development in fish. Mol. Reprod. Dev. 80: 358–370, 2013. © 2013 Wiley Periodicals, Inc . Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library Molecular Reproduction and Development 80 5 358 370
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collection Wiley Online Library
op_collection_id crwiley
language English
description Abstract The factors of the Sox9‐Amh‐Cyp19a1 cascade play a crucial role in the complex process of sex differentiation in mammals. The involvement of Sox9 and Cyp19a1 paralogs and the single Amh ortholog in sex differentiation and development of the gonads and the brain in Atlantic cod was examined by analyzing bimodal and sex‐dimorphic gene expression patterns, respectively, during early stages and in maturing males and females. Expression of sox9a and sox9b were initiated at blastulation, and both paralogs were expressed in chondrogenic tissue in the hatched larvae. The male‐specific expression of sox9a in the adult gonads supports a conserved role in testis function, while sox9b was expressed in the maturing testes and ovaries at similar levels. Amh was expressed at low, but variable, levels from late gastrulation prior to the onset of cyp19a1a and cyp19a1b expression. Male‐biased amh expression was found in the maturing gonads, but the increased ovarian levels during maturation suggest a role also in females. The larval expression of cyp19a1a and cyp19a1b increased at the expected time of sex differentiation, but showed large individual variation. The ovarian expression of cyp19a1a and amh increased concomitant with increased plasma estradiol levels during vitellogenesis. The testis‐specific cyp19a1b expression supports the importance of estrogen in the spermatogenesis, while abundant expression in the male and female brain is probably related to the continuous neurogenesis in fish. These divergent and sex‐dimorphic expression patterns of the cod sox9 and cyp19a1 paralogs demonstrate the complexity of the genetic network regulating sexual development in fish. Mol. Reprod. Dev. 80: 358–370, 2013. © 2013 Wiley Periodicals, Inc .
author2 The Research Council of Norway
format Article in Journal/Newspaper
author Johnsen, Hanne
Tveiten, Helge
Torgersen, Jacob S.
Andersen, Øivind
spellingShingle Johnsen, Hanne
Tveiten, Helge
Torgersen, Jacob S.
Andersen, Øivind
Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.)
author_facet Johnsen, Hanne
Tveiten, Helge
Torgersen, Jacob S.
Andersen, Øivind
author_sort Johnsen, Hanne
title Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.)
title_short Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.)
title_full Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.)
title_fullStr Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.)
title_full_unstemmed Divergent and sex‐dimorphic expression of the paralogs of the Sox9‐Amh‐Cyp19a1 regulatory cascade in developing and adult atlantic cod ( Gadus morhua L.)
title_sort divergent and sex‐dimorphic expression of the paralogs of the sox9‐amh‐cyp19a1 regulatory cascade in developing and adult atlantic cod ( gadus morhua l.)
publisher Wiley
publishDate 2013
url http://dx.doi.org/10.1002/mrd.22170
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.22170
https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22170
genre atlantic cod
Gadus morhua
genre_facet atlantic cod
Gadus morhua
op_source Molecular Reproduction and Development
volume 80, issue 5, page 358-370
ISSN 1040-452X 1098-2795
op_rights http://onlinelibrary.wiley.com/termsAndConditions#vor
op_doi https://doi.org/10.1002/mrd.22170
container_title Molecular Reproduction and Development
container_volume 80
container_issue 5
container_start_page 358
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