Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii
Abstract The molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. The sex differentiation period was investigated in the Siberian sturgeon, Acipenser baerii , by expression profiling of Sertoli cell transcription factors ( dmrt1 , sox9 ) tha...
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crwiley:10.1002/mrd.22053 2024-06-23T07:44:53+00:00 Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii Berbejillo, Julio Martinez‐Bengochea, Anabel Bedo, Gabriela Brunet, Frédéric Volff, Jean‐Nicolas Vizziano‐Cantonnet, Denise 2012 http://dx.doi.org/10.1002/mrd.22053 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.22053 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22053 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Reproduction and Development volume 79, issue 8, page 504-516 ISSN 1040-452X 1098-2795 journal-article 2012 crwiley https://doi.org/10.1002/mrd.22053 2024-06-04T06:44:19Z Abstract The molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. The sex differentiation period was investigated in the Siberian sturgeon, Acipenser baerii , by expression profiling of Sertoli cell transcription factors ( dmrt1 , sox9 ) that control testis differentiation in vertebrates; Leydig cell factors ( cyp17a1 , star ) affecting androgen production; the androgen receptor ( ar ); a growth factor controlling testis development ( igf1 ); and a gene coding for a gonadotropin hormone ( lh ). Two genes were characterised for the first time in the Siberian sturgeon ( dmrt1 , cyp17a1 ), while the others came from public databases. Sturgeon gonad development is very slow, with a late sexual differentiation time during their juvenile stage, and are still immature at 3 years of age. Immature fish showed a sex‐dimorphic pattern; all the genes studied displayed a higher expression level in male gonads. We took advantage of the presence of juvenile fish with pre‐ and post‐differentiated gonads (16 and 18 months old) to characterise them at the molecular level. The post‐differentiated fish displayed a sex dimorphism of gene expression in their gonads for all genes studied, with the exception of sox9 . The trends in undifferentiated fish lead us to propose that sturgeons undergoing male differentiation express high levels of Sertoli cell factors ( dmrt1 , sox9 ) and of genes involved in the production and receptivity of androgens ( cyp17a1 , star and ar ) together with lh . Expression profiles and phylogenetic studies suggest that these genes are potential regulators of testis development in the Siberian sturgeon.Mol. Reprod. Dev. 79: 504‐504, 2012. © 2012 Wiley Periodicals, Inc. Article in Journal/Newspaper Acipenser baerii Siberian sturgeon Wiley Online Library Molecular Reproduction and Development 79 8 504 516 |
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English |
description |
Abstract The molecular mechanisms underlying testis differentiation in basal actinopterygian fish remains poorly understood. The sex differentiation period was investigated in the Siberian sturgeon, Acipenser baerii , by expression profiling of Sertoli cell transcription factors ( dmrt1 , sox9 ) that control testis differentiation in vertebrates; Leydig cell factors ( cyp17a1 , star ) affecting androgen production; the androgen receptor ( ar ); a growth factor controlling testis development ( igf1 ); and a gene coding for a gonadotropin hormone ( lh ). Two genes were characterised for the first time in the Siberian sturgeon ( dmrt1 , cyp17a1 ), while the others came from public databases. Sturgeon gonad development is very slow, with a late sexual differentiation time during their juvenile stage, and are still immature at 3 years of age. Immature fish showed a sex‐dimorphic pattern; all the genes studied displayed a higher expression level in male gonads. We took advantage of the presence of juvenile fish with pre‐ and post‐differentiated gonads (16 and 18 months old) to characterise them at the molecular level. The post‐differentiated fish displayed a sex dimorphism of gene expression in their gonads for all genes studied, with the exception of sox9 . The trends in undifferentiated fish lead us to propose that sturgeons undergoing male differentiation express high levels of Sertoli cell factors ( dmrt1 , sox9 ) and of genes involved in the production and receptivity of androgens ( cyp17a1 , star and ar ) together with lh . Expression profiles and phylogenetic studies suggest that these genes are potential regulators of testis development in the Siberian sturgeon.Mol. Reprod. Dev. 79: 504‐504, 2012. © 2012 Wiley Periodicals, Inc. |
format |
Article in Journal/Newspaper |
author |
Berbejillo, Julio Martinez‐Bengochea, Anabel Bedo, Gabriela Brunet, Frédéric Volff, Jean‐Nicolas Vizziano‐Cantonnet, Denise |
spellingShingle |
Berbejillo, Julio Martinez‐Bengochea, Anabel Bedo, Gabriela Brunet, Frédéric Volff, Jean‐Nicolas Vizziano‐Cantonnet, Denise Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii |
author_facet |
Berbejillo, Julio Martinez‐Bengochea, Anabel Bedo, Gabriela Brunet, Frédéric Volff, Jean‐Nicolas Vizziano‐Cantonnet, Denise |
author_sort |
Berbejillo, Julio |
title |
Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii |
title_short |
Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii |
title_full |
Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii |
title_fullStr |
Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii |
title_full_unstemmed |
Expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the Siberian sturgeon, Acipenser baerii |
title_sort |
expression and phylogeny of candidate genes for sex differentiation in a primitive fish species, the siberian sturgeon, acipenser baerii |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1002/mrd.22053 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.22053 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.22053 |
genre |
Acipenser baerii Siberian sturgeon |
genre_facet |
Acipenser baerii Siberian sturgeon |
op_source |
Molecular Reproduction and Development volume 79, issue 8, page 504-516 ISSN 1040-452X 1098-2795 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/mrd.22053 |
container_title |
Molecular Reproduction and Development |
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79 |
container_issue |
8 |
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504 |
op_container_end_page |
516 |
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1802638353891328000 |