Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations
Abstract Lipophilic persistent organic pollutants (POPs) are ubiquitous in the environment and suspected to interfere with hormone activities and reproduction. In previous studies we demonstrated that POP exposure can affect sperm DNA integrity and differences between Inuits and Europeans in sperm D...
Published in: | Molecular Reproduction and Development |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2007
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/mrd.20747 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.20747 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.20747 |
id |
crwiley:10.1002/mrd.20747 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/mrd.20747 2024-05-19T07:41:31+00:00 Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations Krüger, Tanja Spanò, Marcello Long, Manhai Eleuteri, Patrizia Rescia, Michele Hjelmborg, Philip S. Manicardi, Gian‐Carlo Bizzaro, Davide Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld‐Jorgensen, Eva C. 2007 http://dx.doi.org/10.1002/mrd.20747 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.20747 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.20747 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Reproduction and Development volume 75, issue 4, page 669-680 ISSN 1040-452X 1098-2795 journal-article 2007 crwiley https://doi.org/10.1002/mrd.20747 2024-05-03T10:59:33Z Abstract Lipophilic persistent organic pollutants (POPs) are ubiquitous in the environment and suspected to interfere with hormone activities and reproduction. In previous studies we demonstrated that POP exposure can affect sperm DNA integrity and differences between Inuits and Europeans in sperm DNA integrity and xenobiotic activity were observed. The aim of this study was to investigate possible relations between human sperm chromatin integrity and the xenobiotic serum activity of lipophilic POPs assessed as effects on the estrogen (ER), androgen (AR), and/or aryl hydrocarbon (AhR) receptors. Human sperm chromatin integrity was assessed as DNA fragmentation index (%DFI) and high DNA stainability (%HDS) using the flow cytometric sperm chromatin structure assay (SCSA). Xenobiotic receptor activities were determined using chemically activated luciferase gene expression (CALUX) assay. The study included 53 Greenlandic Inuits and 247 Europeans (Sweden, Warsaw (Poland) and Kharkiv (Ukraine)). A heterogeneous pattern of correlations was found. For Inuits, ER and AhR activities and %DFI were inversely correlated, whereas a positive correlation between AR activity and %DFI was found for Europeans. In contrast, no correlation between receptor activities and %HDS was observed for Inuits but for Europeans positive and negative correlations were observed between ER and AR activities and %HDS, respectively. We suggest that the different patterns of xenobiotic serum activities, in combination with diet associated factors and/or genetics, might be connected to the observed differences in sperm chromatin integrity between the Inuits and Europeans. Mol. Reprod. Dev. 75: 669–680, 2008. © 2007 Wiley‐Liss, Inc. Article in Journal/Newspaper greenlandic inuit inuits Wiley Online Library Molecular Reproduction and Development 75 4 669 680 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Lipophilic persistent organic pollutants (POPs) are ubiquitous in the environment and suspected to interfere with hormone activities and reproduction. In previous studies we demonstrated that POP exposure can affect sperm DNA integrity and differences between Inuits and Europeans in sperm DNA integrity and xenobiotic activity were observed. The aim of this study was to investigate possible relations between human sperm chromatin integrity and the xenobiotic serum activity of lipophilic POPs assessed as effects on the estrogen (ER), androgen (AR), and/or aryl hydrocarbon (AhR) receptors. Human sperm chromatin integrity was assessed as DNA fragmentation index (%DFI) and high DNA stainability (%HDS) using the flow cytometric sperm chromatin structure assay (SCSA). Xenobiotic receptor activities were determined using chemically activated luciferase gene expression (CALUX) assay. The study included 53 Greenlandic Inuits and 247 Europeans (Sweden, Warsaw (Poland) and Kharkiv (Ukraine)). A heterogeneous pattern of correlations was found. For Inuits, ER and AhR activities and %DFI were inversely correlated, whereas a positive correlation between AR activity and %DFI was found for Europeans. In contrast, no correlation between receptor activities and %HDS was observed for Inuits but for Europeans positive and negative correlations were observed between ER and AR activities and %HDS, respectively. We suggest that the different patterns of xenobiotic serum activities, in combination with diet associated factors and/or genetics, might be connected to the observed differences in sperm chromatin integrity between the Inuits and Europeans. Mol. Reprod. Dev. 75: 669–680, 2008. © 2007 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Krüger, Tanja Spanò, Marcello Long, Manhai Eleuteri, Patrizia Rescia, Michele Hjelmborg, Philip S. Manicardi, Gian‐Carlo Bizzaro, Davide Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld‐Jorgensen, Eva C. |
spellingShingle |
Krüger, Tanja Spanò, Marcello Long, Manhai Eleuteri, Patrizia Rescia, Michele Hjelmborg, Philip S. Manicardi, Gian‐Carlo Bizzaro, Davide Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld‐Jorgensen, Eva C. Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations |
author_facet |
Krüger, Tanja Spanò, Marcello Long, Manhai Eleuteri, Patrizia Rescia, Michele Hjelmborg, Philip S. Manicardi, Gian‐Carlo Bizzaro, Davide Giwercman, Alexander Toft, Gunnar Bonde, Jens Peter Bonefeld‐Jorgensen, Eva C. |
author_sort |
Krüger, Tanja |
title |
Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations |
title_short |
Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations |
title_full |
Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations |
title_fullStr |
Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations |
title_full_unstemmed |
Xenobiotic activity in serum and sperm chromatin integrity in European and inuit populations |
title_sort |
xenobiotic activity in serum and sperm chromatin integrity in european and inuit populations |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1002/mrd.20747 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmrd.20747 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mrd.20747 |
genre |
greenlandic inuit inuits |
genre_facet |
greenlandic inuit inuits |
op_source |
Molecular Reproduction and Development volume 75, issue 4, page 669-680 ISSN 1040-452X 1098-2795 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/mrd.20747 |
container_title |
Molecular Reproduction and Development |
container_volume |
75 |
container_issue |
4 |
container_start_page |
669 |
op_container_end_page |
680 |
_version_ |
1799481115991343104 |