Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns
Scope IgE‐epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide‐based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parv...
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Online Access: | http://dx.doi.org/10.1002/mnfr.201200332 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmnfr.201200332 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mnfr.201200332 |
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crwiley:10.1002/mnfr.201200332 2024-06-23T07:51:03+00:00 Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns Perez‐Gordo, Marina Pastor‐Vargas, Carlos Lin, Jing Bardina, Ludmilla Cases, Barbara Ibáñez, Maria Dolores Vivanco, Fernando Cuesta‐Herranz, Javier Sampson, Hugh A. RETIC RIRAAF Ministerio de Educacion y Ciencia 2013 http://dx.doi.org/10.1002/mnfr.201200332 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmnfr.201200332 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mnfr.201200332 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Nutrition & Food Research volume 57, issue 7, page 1283-1290 ISSN 1613-4125 1613-4133 journal-article 2013 crwiley https://doi.org/10.1002/mnfr.201200332 2024-06-11T04:41:32Z Scope IgE‐epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide‐based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1 ) , the most consumed cod species in Spain. Methods and results Sera from 13 fish‐allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen‐specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod ( Gadus callarias ). In the 3D model of the protein, this region was located on the surface of the protein. Conclusion We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools. Article in Journal/Newspaper atlantic cod Wiley Online Library Molecular Nutrition & Food Research 57 7 1283 1290 |
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Wiley Online Library |
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language |
English |
description |
Scope IgE‐epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide‐based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1 ) , the most consumed cod species in Spain. Methods and results Sera from 13 fish‐allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen‐specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod ( Gadus callarias ). In the 3D model of the protein, this region was located on the surface of the protein. Conclusion We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools. |
author2 |
RETIC RIRAAF Ministerio de Educacion y Ciencia |
format |
Article in Journal/Newspaper |
author |
Perez‐Gordo, Marina Pastor‐Vargas, Carlos Lin, Jing Bardina, Ludmilla Cases, Barbara Ibáñez, Maria Dolores Vivanco, Fernando Cuesta‐Herranz, Javier Sampson, Hugh A. |
spellingShingle |
Perez‐Gordo, Marina Pastor‐Vargas, Carlos Lin, Jing Bardina, Ludmilla Cases, Barbara Ibáñez, Maria Dolores Vivanco, Fernando Cuesta‐Herranz, Javier Sampson, Hugh A. Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns |
author_facet |
Perez‐Gordo, Marina Pastor‐Vargas, Carlos Lin, Jing Bardina, Ludmilla Cases, Barbara Ibáñez, Maria Dolores Vivanco, Fernando Cuesta‐Herranz, Javier Sampson, Hugh A. |
author_sort |
Perez‐Gordo, Marina |
title |
Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns |
title_short |
Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns |
title_full |
Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns |
title_fullStr |
Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns |
title_full_unstemmed |
Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns |
title_sort |
epitope mapping of the major allergen from atlantic cod in spanish population reveals different ige‐binding patterns |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1002/mnfr.201200332 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmnfr.201200332 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mnfr.201200332 |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_source |
Molecular Nutrition & Food Research volume 57, issue 7, page 1283-1290 ISSN 1613-4125 1613-4133 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/mnfr.201200332 |
container_title |
Molecular Nutrition & Food Research |
container_volume |
57 |
container_issue |
7 |
container_start_page |
1283 |
op_container_end_page |
1290 |
_version_ |
1802642048377946112 |