Biocatalytic quantification of α‐glucan in marine particulate organic matter
Abstract Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine α‐glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compoun...
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crwiley:10.1002/mbo3.1289 2024-06-09T07:48:17+00:00 Biocatalytic quantification of α‐glucan in marine particulate organic matter Steinke, Nicola Vidal‐Melgosa, Silvia Schultz‐Johansen, Mikkel Hehemann, Jan‐Hendrik Deutsche Forschungsgemeinschaft 2022 http://dx.doi.org/10.1002/mbo3.1289 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mbo3.1289 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mbo3.1289 en eng Wiley http://creativecommons.org/licenses/by/4.0/ MicrobiologyOpen volume 11, issue 3 ISSN 2045-8827 2045-8827 journal-article 2022 crwiley https://doi.org/10.1002/mbo3.1289 2024-05-16T14:24:07Z Abstract Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine α‐glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compounds are likely to account for a high amount of the carbon stored in the oceans they have not been quantified in marine samples so far. Here we present a method to extract and quantify α‐glucans (and compare it with the β‐glucan laminarin) in particulate organic matter from algal cultures and environmental samples using sequential physicochemical extraction and enzymes as α‐glucan‐specific probes. This enzymatic assay is more specific and less susceptible to side reactions than chemical hydrolysis. Using HPAEC‐PAD to detect the hydrolysis products allows for a glycan quantification in particulate marine samples down to a concentration of ≈2 µg/L. We measured glucans in three cultured microalgae as well as in marine particulate organic matter from the North Sea and western North Atlantic Ocean. While the β‐glucan laminarin from diatoms and brown algae is an essential component of marine carbon turnover, our results further indicate the significant contribution of starch‐like α‐glucans to marine particulate organic matter. Henceforth, the combination of glycan‐linkage‐specific enzymes and chromatographic hydrolysis product detection can provide a powerful tool in the exploration of marine glycans and their role in the global carbon cycle. Article in Journal/Newspaper North Atlantic Wiley Online Library MicrobiologyOpen 11 3 |
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Wiley Online Library |
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English |
description |
Abstract Marine algae drive the marine carbon cycle, converting carbon dioxide into organic material. A major component of this produced biomass is a variety of glycans. Marine α‐glucans include a range of storage glycans from red and green algae, bacteria, fungi, and animals. Although these compounds are likely to account for a high amount of the carbon stored in the oceans they have not been quantified in marine samples so far. Here we present a method to extract and quantify α‐glucans (and compare it with the β‐glucan laminarin) in particulate organic matter from algal cultures and environmental samples using sequential physicochemical extraction and enzymes as α‐glucan‐specific probes. This enzymatic assay is more specific and less susceptible to side reactions than chemical hydrolysis. Using HPAEC‐PAD to detect the hydrolysis products allows for a glycan quantification in particulate marine samples down to a concentration of ≈2 µg/L. We measured glucans in three cultured microalgae as well as in marine particulate organic matter from the North Sea and western North Atlantic Ocean. While the β‐glucan laminarin from diatoms and brown algae is an essential component of marine carbon turnover, our results further indicate the significant contribution of starch‐like α‐glucans to marine particulate organic matter. Henceforth, the combination of glycan‐linkage‐specific enzymes and chromatographic hydrolysis product detection can provide a powerful tool in the exploration of marine glycans and their role in the global carbon cycle. |
author2 |
Deutsche Forschungsgemeinschaft |
format |
Article in Journal/Newspaper |
author |
Steinke, Nicola Vidal‐Melgosa, Silvia Schultz‐Johansen, Mikkel Hehemann, Jan‐Hendrik |
spellingShingle |
Steinke, Nicola Vidal‐Melgosa, Silvia Schultz‐Johansen, Mikkel Hehemann, Jan‐Hendrik Biocatalytic quantification of α‐glucan in marine particulate organic matter |
author_facet |
Steinke, Nicola Vidal‐Melgosa, Silvia Schultz‐Johansen, Mikkel Hehemann, Jan‐Hendrik |
author_sort |
Steinke, Nicola |
title |
Biocatalytic quantification of α‐glucan in marine particulate organic matter |
title_short |
Biocatalytic quantification of α‐glucan in marine particulate organic matter |
title_full |
Biocatalytic quantification of α‐glucan in marine particulate organic matter |
title_fullStr |
Biocatalytic quantification of α‐glucan in marine particulate organic matter |
title_full_unstemmed |
Biocatalytic quantification of α‐glucan in marine particulate organic matter |
title_sort |
biocatalytic quantification of α‐glucan in marine particulate organic matter |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1002/mbo3.1289 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mbo3.1289 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mbo3.1289 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
MicrobiologyOpen volume 11, issue 3 ISSN 2045-8827 2045-8827 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/mbo3.1289 |
container_title |
MicrobiologyOpen |
container_volume |
11 |
container_issue |
3 |
_version_ |
1801379932794781696 |