Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica
Abstract Snow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser e...
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crwiley:10.1002/mbo3.1152 2024-09-15T17:44:54+00:00 Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica Smirnova, Margarita Miamin, Uladzislau Kohler, Achim Valentovich, Leonid Akhremchuk, Artur Sidarenka, Anastasiya Dolgikh, Andrey Shapaval, Volha 2020 http://dx.doi.org/10.1002/mbo3.1152 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mbo3.1152 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mbo3.1152 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ MicrobiologyOpen volume 10, issue 1 ISSN 2045-8827 2045-8827 journal-article 2020 crwiley https://doi.org/10.1002/mbo3.1152 2024-08-13T04:14:10Z Abstract Snow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser extent. In this study, we performed 16S rRNA gene amplicon sequencing of two green snow samples collected from the coastal area of the eastern part of Antarctica and conducted genotypic and phenotypic profiling of 45 fast‐growing bacteria isolated from these samples. 16S rRNA gene amplicon sequencing of two green snow samples showed that bacteria inhabiting these samples are mostly represented by families Burkholderiaceae (46.31%), Flavobacteriaceae (22.98%), and Pseudomonadaceae (17.66%). Identification of 45 fast‐growing bacteria isolated from green snow was performed using 16S rRNA gene sequencing. We demonstrated that they belong to the phyla Actinobacteria and Proteobacteria , and are represented by the genera Arthrobacter , Cryobacterium , Leifsonia , Salinibacterium , Paeniglutamicibacter , Rhodococcu s, Polaromonas , Pseudomonas , and Psychrobacter . Nearly all bacterial isolates exhibited various growth temperatures from 4°C to 25°C, and some isolates were characterized by a high level of enzymatic activity. Phenotyping using Fourier transform infrared (FTIR) spectroscopy revealed a possible accumulation of intracellular polymer polyhydroxyalkanoates (PHA) or lipids in some isolates. The bacteria showed different lipids/PHA and protein profiles. It was shown that lipid/PHA and protein spectral regions are the most discriminative for differentiating the isolates. Article in Journal/Newspaper Antarc* Antarctic Antarctica Climate change East Antarctica Wiley Online Library MicrobiologyOpen 10 1 |
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English |
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Abstract Snow microorganisms play a significant role in climate change and affecting the snow melting rate in the Arctic and Antarctic regions. While research on algae inhabiting green and red snow has been performed extensively, bacteria dwelling in this biotope have been studied to a much lesser extent. In this study, we performed 16S rRNA gene amplicon sequencing of two green snow samples collected from the coastal area of the eastern part of Antarctica and conducted genotypic and phenotypic profiling of 45 fast‐growing bacteria isolated from these samples. 16S rRNA gene amplicon sequencing of two green snow samples showed that bacteria inhabiting these samples are mostly represented by families Burkholderiaceae (46.31%), Flavobacteriaceae (22.98%), and Pseudomonadaceae (17.66%). Identification of 45 fast‐growing bacteria isolated from green snow was performed using 16S rRNA gene sequencing. We demonstrated that they belong to the phyla Actinobacteria and Proteobacteria , and are represented by the genera Arthrobacter , Cryobacterium , Leifsonia , Salinibacterium , Paeniglutamicibacter , Rhodococcu s, Polaromonas , Pseudomonas , and Psychrobacter . Nearly all bacterial isolates exhibited various growth temperatures from 4°C to 25°C, and some isolates were characterized by a high level of enzymatic activity. Phenotyping using Fourier transform infrared (FTIR) spectroscopy revealed a possible accumulation of intracellular polymer polyhydroxyalkanoates (PHA) or lipids in some isolates. The bacteria showed different lipids/PHA and protein profiles. It was shown that lipid/PHA and protein spectral regions are the most discriminative for differentiating the isolates. |
format |
Article in Journal/Newspaper |
author |
Smirnova, Margarita Miamin, Uladzislau Kohler, Achim Valentovich, Leonid Akhremchuk, Artur Sidarenka, Anastasiya Dolgikh, Andrey Shapaval, Volha |
spellingShingle |
Smirnova, Margarita Miamin, Uladzislau Kohler, Achim Valentovich, Leonid Akhremchuk, Artur Sidarenka, Anastasiya Dolgikh, Andrey Shapaval, Volha Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
author_facet |
Smirnova, Margarita Miamin, Uladzislau Kohler, Achim Valentovich, Leonid Akhremchuk, Artur Sidarenka, Anastasiya Dolgikh, Andrey Shapaval, Volha |
author_sort |
Smirnova, Margarita |
title |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_short |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_full |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_fullStr |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_full_unstemmed |
Isolation and characterization of fast‐growing green snow bacteria from coastal East Antarctica |
title_sort |
isolation and characterization of fast‐growing green snow bacteria from coastal east antarctica |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1002/mbo3.1152 https://onlinelibrary.wiley.com/doi/pdf/10.1002/mbo3.1152 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/mbo3.1152 |
genre |
Antarc* Antarctic Antarctica Climate change East Antarctica |
genre_facet |
Antarc* Antarctic Antarctica Climate change East Antarctica |
op_source |
MicrobiologyOpen volume 10, issue 1 ISSN 2045-8827 2045-8827 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1002/mbo3.1152 |
container_title |
MicrobiologyOpen |
container_volume |
10 |
container_issue |
1 |
_version_ |
1810492579353985024 |