Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles
Abstract Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal‐B) and on the hydrolytic activity (hydrolysis of p ‐nitrophenyl acetate) of...
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crwiley:10.1002/marc.200900497 2024-06-23T07:47:44+00:00 Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles Miletić, Nemanja Abetz, Volker Ebert, Katrin Loos, Katja 2009 http://dx.doi.org/10.1002/marc.200900497 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmarc.200900497 https://onlinelibrary.wiley.com/doi/pdf/10.1002/marc.200900497 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Macromolecular Rapid Communications volume 31, issue 1, page 71-74 ISSN 1022-1336 1521-3927 journal-article 2009 crwiley https://doi.org/10.1002/marc.200900497 2024-06-13T04:21:34Z Abstract Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal‐B) and on the hydrolytic activity (hydrolysis of p ‐nitrophenyl acetate) of the immobilized Cal‐B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal‐B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. magnified image Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Macromolecular Rapid Communications 31 1 71 74 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Polystyrene (PS) nanoparticles were prepared via a nanoprecipitation process. The influence of the pH of the buffer solution used during the immobilization process on the loading of Candida antarctica lipase B (Cal‐B) and on the hydrolytic activity (hydrolysis of p ‐nitrophenyl acetate) of the immobilized Cal‐B was studied. The pH of the buffer solution has no influence on enzyme loading, while immobilized enzyme activity is very dependent on the pH of adsorption. Cal‐B immobilized on PS nanoparticles in buffer solution pH 6.8 performed higher hydrolytic activity than crude enzyme powder and Novozyme 435. magnified image |
format |
Article in Journal/Newspaper |
author |
Miletić, Nemanja Abetz, Volker Ebert, Katrin Loos, Katja |
spellingShingle |
Miletić, Nemanja Abetz, Volker Ebert, Katrin Loos, Katja Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
author_facet |
Miletić, Nemanja Abetz, Volker Ebert, Katrin Loos, Katja |
author_sort |
Miletić, Nemanja |
title |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_short |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_full |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_fullStr |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_full_unstemmed |
Immobilization of Candida antarctica lipase B on Polystyrene Nanoparticles |
title_sort |
immobilization of candida antarctica lipase b on polystyrene nanoparticles |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1002/marc.200900497 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmarc.200900497 https://onlinelibrary.wiley.com/doi/pdf/10.1002/marc.200900497 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Macromolecular Rapid Communications volume 31, issue 1, page 71-74 ISSN 1022-1336 1521-3927 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/marc.200900497 |
container_title |
Macromolecular Rapid Communications |
container_volume |
31 |
container_issue |
1 |
container_start_page |
71 |
op_container_end_page |
74 |
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1802651886867709952 |