Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B
Abstract PANGMA nanofibers and nanomats with fiber diameters of 200–300 nanometers were fabricated by electrospinning. Cal‐B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal‐B‐immobilized PANGMA nanomats were assayed and compare...
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crwiley:10.1002/macp.201000536 2024-09-15T17:46:26+00:00 Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B Dai, Tianhe Miletić, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker 2010 http://dx.doi.org/10.1002/macp.201000536 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmacp.201000536 https://onlinelibrary.wiley.com/doi/pdf/10.1002/macp.201000536 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Macromolecular Chemistry and Physics volume 212, issue 4, page 319-327 ISSN 1022-1352 1521-3935 journal-article 2010 crwiley https://doi.org/10.1002/macp.201000536 2024-07-18T04:25:48Z Abstract PANGMA nanofibers and nanomats with fiber diameters of 200–300 nanometers were fabricated by electrospinning. Cal‐B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal‐B‐immobilized PANGMA nanomats were assayed and compared with the free Cal‐B. The observed Cal‐B loading on these nanomats is up to ≈50 mg · g −1 , and their hydrolytic activity is up to ≈2 500 nmol · min −1 · mg −1 , much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal‐B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal‐B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage. magnified image Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Macromolecular Chemistry and Physics 212 4 319 327 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract PANGMA nanofibers and nanomats with fiber diameters of 200–300 nanometers were fabricated by electrospinning. Cal‐B was covalently immobilized onto the PANGMA nanomats via three different immobilization routes. The properties of the Cal‐B‐immobilized PANGMA nanomats were assayed and compared with the free Cal‐B. The observed Cal‐B loading on these nanomats is up to ≈50 mg · g −1 , and their hydrolytic activity is up to ≈2 500 nmol · min −1 · mg −1 , much higher than free enzyme powder and also slightly higher than Novozyme 435. Cal‐B immobilized PANGMA nanomats have better reusability, thermal stability, and storage ability than free Cal‐B. They retain over 50% of their initial activity after 15 cycles, over 65% after 10 h heat incubation, and over 75% after 30 d storage. magnified image |
format |
Article in Journal/Newspaper |
author |
Dai, Tianhe Miletić, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker |
spellingShingle |
Dai, Tianhe Miletić, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
author_facet |
Dai, Tianhe Miletić, Nemanja Loos, Katja Elbahri, Mady Abetz, Volker |
author_sort |
Dai, Tianhe |
title |
Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_short |
Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_full |
Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_fullStr |
Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_full_unstemmed |
Electrospinning of Poly[acrylonitrile‐co‐(glycidyl methacrylate)] Nanofibrous Mats for the Immobilization of Candida Antarctica Lipase B |
title_sort |
electrospinning of poly[acrylonitrile‐co‐(glycidyl methacrylate)] nanofibrous mats for the immobilization of candida antarctica lipase b |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1002/macp.201000536 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fmacp.201000536 https://onlinelibrary.wiley.com/doi/pdf/10.1002/macp.201000536 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Macromolecular Chemistry and Physics volume 212, issue 4, page 319-327 ISSN 1022-1352 1521-3935 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/macp.201000536 |
container_title |
Macromolecular Chemistry and Physics |
container_volume |
212 |
container_issue |
4 |
container_start_page |
319 |
op_container_end_page |
327 |
_version_ |
1810494567410040832 |