FIRe glider: Mapping in situ chlorophyll variable fluorescence with autonomous underwater gliders

• Published in: Limnology and Oceanography: Methods
• Main Authors: Carvalho, Filipa, Gorbunov, Maxim Y., Oliver, Matthew J., Haskins, Christina, Aragon, David, Kohut, Josh T., Schofield, Oscar
• Other Authors: Fundação para a Ciência e a Tecnologia, H2020 European Research Council, National Aeronautics and Space Administration, Office of Polar Programs
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2020
• Subjects: Southern Ocean
• Online Access: http://dx.doi.org/10.1002/lom3.10380; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flom3.10380; https://onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10380; https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lom3.10380; https://onlinelibrary.wiley.com/doi/am-pdf/10.1002%2Flom3.10380; https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10380

Abstract Nutrient and light availability regulate phytoplankton physiology and photosynthesis in the ocean. These physiological processes are difficult to sample in time and space over physiologically and ecologically relevant scales using traditional shipboard techniques. Gliders are changing the nature of data collection, by allowing a sustained presence at sea over regional scales, collecting data at resolution not possible using traditional techniques. The integration of a fluorescence induction and relaxation (FIRe) sensor in a Slocum glider allows autonomous high‐resolution and vertically‐resolved measurements of photosynthetic physiological variables together with oceanographic data. In situ measurements of variable fluorescence under ambient light allows a better understanding of the physical controls of primary production (PP). We demonstrate this capability in a laboratory setting and with several glider deployments in the Southern Ocean. Development of these approaches will allow for the in situ evaluation of phytoplankton light stress and photoacclimation mechanisms, as well as the role of vertical mixing in phytoplankton dynamics and the underlying physiology, especially in remote locations and for prolonged duration.