Measurement of methylamines in seawater using solid phase microextraction and gas chromatography
Abstract The methylamines form part of the marine organic nitrogen pool. Although they are ubiquitous in marine systems, little is known about their distribution, production, and fate in the marine environment. Analytical methods have been developed for their analysis in marine waters, but these hav...
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crwiley:10.1002/lom3.10255 2024-06-23T07:56:56+00:00 Measurement of methylamines in seawater using solid phase microextraction and gas chromatography Cree, Charlotte H. L. Airs, Ruth Archer, Stephen D. Fitzsimons, Mark F. Natural Environment Research Council 2018 http://dx.doi.org/10.1002/lom3.10255 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flom3.10255 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10255 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography: Methods volume 16, issue 7, page 411-420 ISSN 1541-5856 1541-5856 journal-article 2018 crwiley https://doi.org/10.1002/lom3.10255 2024-06-06T04:21:51Z Abstract The methylamines form part of the marine organic nitrogen pool. Although they are ubiquitous in marine systems, little is known about their distribution, production, and fate in the marine environment. Analytical methods have been developed for their analysis in marine waters, but these have employed custom‐made apparatus, limiting data to a few studies. Here, we report a method developed for the determination of methylamines in seawater which combines headspace solid phase microextraction (SPME) and gas chromatography with selective nitrogen detection. Gaseous methylamines were pre‐concentrated from water samples (∼ 1 L) heated to 60 °C, onto a polydimethylsiloxane/divinylbenzene SPME fiber located in the headspace of the extraction flask. The analytes were then thermally desorbed from the fiber in a gas chromatograph containing a CP‐Volamine column and a nitrogen–phosphorus detector. The method was routinely calibrated down to 1 nM for all three analytes using matrix‐matched external standards. Limits of detection were determined operationally and ranged from 0.4 nM to 2.9 nM during two sampling campaigns. The analytes were detected in seawater samples from the English Channel and Southern Ocean where concentrations up to 20 nM and 5.6 nM were measured, respectively, consistent with previously reported levels. We propose this method for the analysis of methylamines, and other low‐molecular weight amines, as it is sensitive, robust, and the necessary equipment is widely available. Article in Journal/Newspaper Southern Ocean Wiley Online Library Southern Ocean Limnology and Oceanography: Methods 16 7 411 420 |
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Open Polar |
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Wiley Online Library |
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English |
description |
Abstract The methylamines form part of the marine organic nitrogen pool. Although they are ubiquitous in marine systems, little is known about their distribution, production, and fate in the marine environment. Analytical methods have been developed for their analysis in marine waters, but these have employed custom‐made apparatus, limiting data to a few studies. Here, we report a method developed for the determination of methylamines in seawater which combines headspace solid phase microextraction (SPME) and gas chromatography with selective nitrogen detection. Gaseous methylamines were pre‐concentrated from water samples (∼ 1 L) heated to 60 °C, onto a polydimethylsiloxane/divinylbenzene SPME fiber located in the headspace of the extraction flask. The analytes were then thermally desorbed from the fiber in a gas chromatograph containing a CP‐Volamine column and a nitrogen–phosphorus detector. The method was routinely calibrated down to 1 nM for all three analytes using matrix‐matched external standards. Limits of detection were determined operationally and ranged from 0.4 nM to 2.9 nM during two sampling campaigns. The analytes were detected in seawater samples from the English Channel and Southern Ocean where concentrations up to 20 nM and 5.6 nM were measured, respectively, consistent with previously reported levels. We propose this method for the analysis of methylamines, and other low‐molecular weight amines, as it is sensitive, robust, and the necessary equipment is widely available. |
author2 |
Natural Environment Research Council |
format |
Article in Journal/Newspaper |
author |
Cree, Charlotte H. L. Airs, Ruth Archer, Stephen D. Fitzsimons, Mark F. |
spellingShingle |
Cree, Charlotte H. L. Airs, Ruth Archer, Stephen D. Fitzsimons, Mark F. Measurement of methylamines in seawater using solid phase microextraction and gas chromatography |
author_facet |
Cree, Charlotte H. L. Airs, Ruth Archer, Stephen D. Fitzsimons, Mark F. |
author_sort |
Cree, Charlotte H. L. |
title |
Measurement of methylamines in seawater using solid phase microextraction and gas chromatography |
title_short |
Measurement of methylamines in seawater using solid phase microextraction and gas chromatography |
title_full |
Measurement of methylamines in seawater using solid phase microextraction and gas chromatography |
title_fullStr |
Measurement of methylamines in seawater using solid phase microextraction and gas chromatography |
title_full_unstemmed |
Measurement of methylamines in seawater using solid phase microextraction and gas chromatography |
title_sort |
measurement of methylamines in seawater using solid phase microextraction and gas chromatography |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/lom3.10255 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flom3.10255 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10255 |
geographic |
Southern Ocean |
geographic_facet |
Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Limnology and Oceanography: Methods volume 16, issue 7, page 411-420 ISSN 1541-5856 1541-5856 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/lom3.10255 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
16 |
container_issue |
7 |
container_start_page |
411 |
op_container_end_page |
420 |
_version_ |
1802650331770781696 |