Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples
Abstract A microwave‐preservation method was developed to quantify total dimethylsulfoniopropionate (DMSP T ), dimethylsulfoxide (DMSO T ), and acrylate (acrylate T ) concentrations in unfiltered samples to alleviate problems associated with the acidification method when applied to samples containin...
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crwiley:10.1002/lom3.10081 2024-06-02T07:58:08+00:00 Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples Kinsey, Joanna D. Kieber, David J. 2016 http://dx.doi.org/10.1002/lom3.10081 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flom3.10081 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10081 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography: Methods volume 14, issue 3, page 196-209 ISSN 1541-5856 1541-5856 journal-article 2016 crwiley https://doi.org/10.1002/lom3.10081 2024-05-03T11:19:23Z Abstract A microwave‐preservation method was developed to quantify total dimethylsulfoniopropionate (DMSP T ), dimethylsulfoxide (DMSO T ), and acrylate (acrylate T ) concentrations in unfiltered samples to alleviate problems associated with the acidification method when applied to samples containing Phaeocystis . Microwave‐ and acid‐preservation methods were compared using batch cultures of Phaeocystis antarctica and 11 other marine phytoplankton species for DMSP T , batch P. antarctica cultures for DMSO T and acrylate T , and unfiltered Delaware Estuary water samples for DMSP T to demonstrate the general applicability of this method. Acidification of P. antarctica culture samples resulted in the underestimation of DMSP T (42–69%) and overestimation of dimethylsulfide (DMS) (2156–3819%), DMSO T (9–101%), and acrylate T (71–249%). By comparison, DMSP T concentrations in microwaved samples agreed with non‐microwaved, non‐acidified controls. In contrast to P. antarctica results, the microwave‐ and acid‐preservation methods yielded DMSP T concentrations that were statistically indistinguishable for 11 other marine phytoplankton species and Delaware Estuary samples. Unfiltered samples stored frozen following microwave treatment or stored at room temperature if acidified after the microwaving step, resulted in no change in DMSP T or acrylate T DMSO T concentrations increased slightly (∼ 15%) when they were not sparged to remove DMS prior to acidification and room temperature storage. Based on these findings, we propose microwaving small sample volumes (≤ 7 mL) of unfiltered seawater or culture samples as a general approach to preserve samples for subsequent DMSP T , DMSO T , and acrylate T analyses, especially when the phytoplankton composition of the samples is unknown. Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Limnology and Oceanography: Methods 14 3 196 209 |
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Wiley Online Library |
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English |
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Abstract A microwave‐preservation method was developed to quantify total dimethylsulfoniopropionate (DMSP T ), dimethylsulfoxide (DMSO T ), and acrylate (acrylate T ) concentrations in unfiltered samples to alleviate problems associated with the acidification method when applied to samples containing Phaeocystis . Microwave‐ and acid‐preservation methods were compared using batch cultures of Phaeocystis antarctica and 11 other marine phytoplankton species for DMSP T , batch P. antarctica cultures for DMSO T and acrylate T , and unfiltered Delaware Estuary water samples for DMSP T to demonstrate the general applicability of this method. Acidification of P. antarctica culture samples resulted in the underestimation of DMSP T (42–69%) and overestimation of dimethylsulfide (DMS) (2156–3819%), DMSO T (9–101%), and acrylate T (71–249%). By comparison, DMSP T concentrations in microwaved samples agreed with non‐microwaved, non‐acidified controls. In contrast to P. antarctica results, the microwave‐ and acid‐preservation methods yielded DMSP T concentrations that were statistically indistinguishable for 11 other marine phytoplankton species and Delaware Estuary samples. Unfiltered samples stored frozen following microwave treatment or stored at room temperature if acidified after the microwaving step, resulted in no change in DMSP T or acrylate T DMSO T concentrations increased slightly (∼ 15%) when they were not sparged to remove DMS prior to acidification and room temperature storage. Based on these findings, we propose microwaving small sample volumes (≤ 7 mL) of unfiltered seawater or culture samples as a general approach to preserve samples for subsequent DMSP T , DMSO T , and acrylate T analyses, especially when the phytoplankton composition of the samples is unknown. |
format |
Article in Journal/Newspaper |
author |
Kinsey, Joanna D. Kieber, David J. |
spellingShingle |
Kinsey, Joanna D. Kieber, David J. Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples |
author_facet |
Kinsey, Joanna D. Kieber, David J. |
author_sort |
Kinsey, Joanna D. |
title |
Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples |
title_short |
Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples |
title_full |
Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples |
title_fullStr |
Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples |
title_full_unstemmed |
Microwave preservation method for DMSP, DMSO, and acrylate in unfiltered seawater and phytoplankton culture samples |
title_sort |
microwave preservation method for dmsp, dmso, and acrylate in unfiltered seawater and phytoplankton culture samples |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1002/lom3.10081 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flom3.10081 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lom3.10081 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Limnology and Oceanography: Methods volume 14, issue 3, page 196-209 ISSN 1541-5856 1541-5856 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/lom3.10081 |
container_title |
Limnology and Oceanography: Methods |
container_volume |
14 |
container_issue |
3 |
container_start_page |
196 |
op_container_end_page |
209 |
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1800741405278076928 |