Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters
Abstract Aquatic microbial responses to changes in the amount and composition of dissolved organic carbon (DOC) are of fundamental ecological and biogeochemical importance. Parallel factor (PARAFAC) analysis of excitation–emission fluorescence spectra is a common tool to characterize DOC, yet its ab...
Published in: | Limnology and Oceanography |
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Online Access: | http://dx.doi.org/10.1002/lno.12406 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12406 |
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crwiley:10.1002/lno.12406 2024-06-02T08:12:12+00:00 Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann‐Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert Knut och Alice Wallenbergs Stiftelse Svenska Forskningsrådet Formas Vetenskapsrådet 2023 http://dx.doi.org/10.1002/lno.12406 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12406 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Limnology and Oceanography volume 68, issue 9, page 2059-2069 ISSN 0024-3590 1939-5590 journal-article 2023 crwiley https://doi.org/10.1002/lno.12406 2024-05-03T10:58:42Z Abstract Aquatic microbial responses to changes in the amount and composition of dissolved organic carbon (DOC) are of fundamental ecological and biogeochemical importance. Parallel factor (PARAFAC) analysis of excitation–emission fluorescence spectra is a common tool to characterize DOC, yet its ability to predict bacterial production (BP), bacterial respiration (BR), and bacterial growth efficiency (BGE) vary widely, potentially because inorganic nutrient limitation decouples microbial processes from their dependence on DOC composition. We used 28‐d bioassays with water from 19 lakes, streams, and rivers in northern Sweden to test how much the links between bacterial metabolism and fluorescence PARAFAC components depend on experimental additions of inorganic nutrients. We found a significant interaction effect between nutrient addition and fluorescence on carbon‐specific BP, and weak evidence for influence on BGE by the same interaction ( p = 0.1), but no corresponding interaction effect on BR. A practical implication of this interaction was that fluorescence components could explain more than twice as much of the variability in carbon‐specific BP ( R 2 = 0.90) and BGE ( R 2 = 0.70) after nitrogen and phosphorus addition, compared with control incubations. Our results suggest that an increased supply of labile DOC relative to ambient phosphorus and nitrogen induces gradually larger degrees of nutrient limitation of BP, which in turn decouple BP and BGE from fluorescence signals. Thus, while fluorescence does contain precise information about the degree to which DOC can support microbial processes, this information may be hidden in field studies due to nutrient limitation of bacterial metabolism. Article in Journal/Newspaper Northern Sweden Wiley Online Library Limnology and Oceanography 68 9 2059 2069 |
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English |
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Abstract Aquatic microbial responses to changes in the amount and composition of dissolved organic carbon (DOC) are of fundamental ecological and biogeochemical importance. Parallel factor (PARAFAC) analysis of excitation–emission fluorescence spectra is a common tool to characterize DOC, yet its ability to predict bacterial production (BP), bacterial respiration (BR), and bacterial growth efficiency (BGE) vary widely, potentially because inorganic nutrient limitation decouples microbial processes from their dependence on DOC composition. We used 28‐d bioassays with water from 19 lakes, streams, and rivers in northern Sweden to test how much the links between bacterial metabolism and fluorescence PARAFAC components depend on experimental additions of inorganic nutrients. We found a significant interaction effect between nutrient addition and fluorescence on carbon‐specific BP, and weak evidence for influence on BGE by the same interaction ( p = 0.1), but no corresponding interaction effect on BR. A practical implication of this interaction was that fluorescence components could explain more than twice as much of the variability in carbon‐specific BP ( R 2 = 0.90) and BGE ( R 2 = 0.70) after nitrogen and phosphorus addition, compared with control incubations. Our results suggest that an increased supply of labile DOC relative to ambient phosphorus and nitrogen induces gradually larger degrees of nutrient limitation of BP, which in turn decouple BP and BGE from fluorescence signals. Thus, while fluorescence does contain precise information about the degree to which DOC can support microbial processes, this information may be hidden in field studies due to nutrient limitation of bacterial metabolism. |
author2 |
Knut och Alice Wallenbergs Stiftelse Svenska Forskningsrådet Formas Vetenskapsrådet |
format |
Article in Journal/Newspaper |
author |
Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann‐Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert |
spellingShingle |
Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann‐Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
author_facet |
Berggren, Martin Ye, Linlin Sponseller, Ryan A. Bergström, Ann‐Kristin Karlsson, Jan Verheijen, Hendricus Hensgens, Geert |
author_sort |
Berggren, Martin |
title |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_short |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_full |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_fullStr |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_full_unstemmed |
Nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
title_sort |
nutrient limitation masks the dissolved organic matter composition effects on bacterial metabolism in unproductive freshwaters |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/lno.12406 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12406 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Limnology and Oceanography volume 68, issue 9, page 2059-2069 ISSN 0024-3590 1939-5590 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/lno.12406 |
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Limnology and Oceanography |
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68 |
container_issue |
9 |
container_start_page |
2059 |
op_container_end_page |
2069 |
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1800758575710076928 |