Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19
Abstract Functional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diat...
Published in: | Limnology and Oceanography |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | http://dx.doi.org/10.1002/lno.12201 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12201 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.12201 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12201 |
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crwiley:10.1002/lno.12201 2024-06-02T07:57:50+00:00 Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19 Kellogg, Riss M. Moran, Dawn M. McIlvin, Matthew R. Subhas, Adam V. Allen, Andrew E. Saito, Mak A. National Institutes of Health National Science Foundation of Sri Lanka 2022 http://dx.doi.org/10.1002/lno.12201 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12201 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.12201 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12201 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 67, issue 10, page 2265-2280 ISSN 0024-3590 1939-5590 journal-article 2022 crwiley https://doi.org/10.1002/lno.12201 2024-05-03T11:45:33Z Abstract Functional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diatom Chaetoceros neogracile RS19, originally isolated from the Ross Sea, Antarctica, has a Zn requirement that cannot be met by Co and thus does not demonstrate a Zn/Co substitution ability as assessed by growth rate. We investigated this diatom's inability to use Co to alleviate Zn‐limited growth rates at the transporter, sensor/chaperone, and metalloenzyme level using metal quota and proteomic analyses of cultures grown over a range of Zn and Co availability. Analysis of total cellular metal quotas revealed that, although incapable of substitution, this diatom still actively assimilated dissolved Co. We furthermore observed distinct trends in the abundance levels of putative α and θ‐CAs, ZIP transporters, Zn fingers, and a Zn chaperone in response to increasing media Zn 2+ . Overall, Co appears to be transported into the cell, but not efficiently utilized by Zn metalloenzymes. Article in Journal/Newspaper Antarc* Antarctica Ross Sea Wiley Online Library Ross Sea Limnology and Oceanography 67 10 2265 2280 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Functional substitution of the essential trace metals zinc (Zn) and cobalt (Co) within metalloenzymes has been well documented in marine diatoms and is known to be prevalent among varying genera and species. In contrast to the majority of species studied to date, we find that the polar diatom Chaetoceros neogracile RS19, originally isolated from the Ross Sea, Antarctica, has a Zn requirement that cannot be met by Co and thus does not demonstrate a Zn/Co substitution ability as assessed by growth rate. We investigated this diatom's inability to use Co to alleviate Zn‐limited growth rates at the transporter, sensor/chaperone, and metalloenzyme level using metal quota and proteomic analyses of cultures grown over a range of Zn and Co availability. Analysis of total cellular metal quotas revealed that, although incapable of substitution, this diatom still actively assimilated dissolved Co. We furthermore observed distinct trends in the abundance levels of putative α and θ‐CAs, ZIP transporters, Zn fingers, and a Zn chaperone in response to increasing media Zn 2+ . Overall, Co appears to be transported into the cell, but not efficiently utilized by Zn metalloenzymes. |
author2 |
National Institutes of Health National Science Foundation of Sri Lanka |
format |
Article in Journal/Newspaper |
author |
Kellogg, Riss M. Moran, Dawn M. McIlvin, Matthew R. Subhas, Adam V. Allen, Andrew E. Saito, Mak A. |
spellingShingle |
Kellogg, Riss M. Moran, Dawn M. McIlvin, Matthew R. Subhas, Adam V. Allen, Andrew E. Saito, Mak A. Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19 |
author_facet |
Kellogg, Riss M. Moran, Dawn M. McIlvin, Matthew R. Subhas, Adam V. Allen, Andrew E. Saito, Mak A. |
author_sort |
Kellogg, Riss M. |
title |
Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19 |
title_short |
Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19 |
title_full |
Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19 |
title_fullStr |
Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19 |
title_full_unstemmed |
Lack of a Zn/Co substitution ability in the polar diatom Chaetoceros neogracile RS19 |
title_sort |
lack of a zn/co substitution ability in the polar diatom chaetoceros neogracile rs19 |
publisher |
Wiley |
publishDate |
2022 |
url |
http://dx.doi.org/10.1002/lno.12201 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12201 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.12201 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.12201 |
geographic |
Ross Sea |
geographic_facet |
Ross Sea |
genre |
Antarc* Antarctica Ross Sea |
genre_facet |
Antarc* Antarctica Ross Sea |
op_source |
Limnology and Oceanography volume 67, issue 10, page 2265-2280 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/lno.12201 |
container_title |
Limnology and Oceanography |
container_volume |
67 |
container_issue |
10 |
container_start_page |
2265 |
op_container_end_page |
2280 |
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1800741045472854016 |