Physiological and molecular responses to ocean acidification among strains of a model diatom
Abstract Differential responses of diatoms, an important group of marine primary producers to ocean acidification, have been well documented. However, studies so far are based on limited representative strains from key species. Investigation of strain level responses will help us better understand t...
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crwiley:10.1002/lno.11565 2024-04-28T08:34:26+00:00 Physiological and molecular responses to ocean acidification among strains of a model diatom Huang, Ruiping Ding, Jiancheng Sun, Jiazhen Tian, Yang Bowler, Chris Lin, Xin Gao, Kunshan National Basic Research Program of China National Natural Science Foundation of China 2020 http://dx.doi.org/10.1002/lno.11565 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11565 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.11565 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11565 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Limnology and Oceanography volume 65, issue 12, page 2926-2936 ISSN 0024-3590 1939-5590 Aquatic Science Oceanography journal-article 2020 crwiley https://doi.org/10.1002/lno.11565 2024-04-08T06:57:09Z Abstract Differential responses of diatoms, an important group of marine primary producers to ocean acidification, have been well documented. However, studies so far are based on limited representative strains from key species. Investigation of strain level responses will help us better understand the contrasting discrepancy in diatom responses to ocean acidification. Here, we selected four strains of the model diatom Phaeodactylum tricornutum isolated from different regions of the global ocean, representing all genotypes based on internal transcribed spacer 2 sequences, and investigated strain‐specific responses to ocean acidification. In response to ocean acidification, changes in carbon metabolism varied among strains, although no significant effects of ocean acidification on growth rates or pigments were observed in any strains. The expression of genes encoding plasma membrane bicarbonate transporters was downregulated in strain Pt4, reflecting a potential decrease in active uptake, which was not observed in the other strains. Reduction of CO 2 concentrating mechanism efficiency was also indicated by the regulated expression of genes encoding carbonic anhydrases that catalyze the interconversion of and CO 2 in the pyrenoids and pyrenoid‐penetrating thylakoid, which exhibited different patterns among the strains. Under ocean acidification conditions, C4‐like metabolism appeared to redistribute carbon flux to gluconeogenesis in strain Pt1, and lipid synthesis in strains Pt8 and Pt11, rather than participating in net photosynthetic carbon fixation. These variations were incompletely correlated with phylogenetic relationship in different strains, implying that the habitat‐adapted imprints of the different strains could also be responsible for their differential responses to ocean acidification. Article in Journal/Newspaper Ocean acidification Wiley Online Library Limnology and Oceanography 65 12 2926 2936 |
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English |
topic |
Aquatic Science Oceanography |
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Aquatic Science Oceanography Huang, Ruiping Ding, Jiancheng Sun, Jiazhen Tian, Yang Bowler, Chris Lin, Xin Gao, Kunshan Physiological and molecular responses to ocean acidification among strains of a model diatom |
topic_facet |
Aquatic Science Oceanography |
description |
Abstract Differential responses of diatoms, an important group of marine primary producers to ocean acidification, have been well documented. However, studies so far are based on limited representative strains from key species. Investigation of strain level responses will help us better understand the contrasting discrepancy in diatom responses to ocean acidification. Here, we selected four strains of the model diatom Phaeodactylum tricornutum isolated from different regions of the global ocean, representing all genotypes based on internal transcribed spacer 2 sequences, and investigated strain‐specific responses to ocean acidification. In response to ocean acidification, changes in carbon metabolism varied among strains, although no significant effects of ocean acidification on growth rates or pigments were observed in any strains. The expression of genes encoding plasma membrane bicarbonate transporters was downregulated in strain Pt4, reflecting a potential decrease in active uptake, which was not observed in the other strains. Reduction of CO 2 concentrating mechanism efficiency was also indicated by the regulated expression of genes encoding carbonic anhydrases that catalyze the interconversion of and CO 2 in the pyrenoids and pyrenoid‐penetrating thylakoid, which exhibited different patterns among the strains. Under ocean acidification conditions, C4‐like metabolism appeared to redistribute carbon flux to gluconeogenesis in strain Pt1, and lipid synthesis in strains Pt8 and Pt11, rather than participating in net photosynthetic carbon fixation. These variations were incompletely correlated with phylogenetic relationship in different strains, implying that the habitat‐adapted imprints of the different strains could also be responsible for their differential responses to ocean acidification. |
author2 |
National Basic Research Program of China National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Huang, Ruiping Ding, Jiancheng Sun, Jiazhen Tian, Yang Bowler, Chris Lin, Xin Gao, Kunshan |
author_facet |
Huang, Ruiping Ding, Jiancheng Sun, Jiazhen Tian, Yang Bowler, Chris Lin, Xin Gao, Kunshan |
author_sort |
Huang, Ruiping |
title |
Physiological and molecular responses to ocean acidification among strains of a model diatom |
title_short |
Physiological and molecular responses to ocean acidification among strains of a model diatom |
title_full |
Physiological and molecular responses to ocean acidification among strains of a model diatom |
title_fullStr |
Physiological and molecular responses to ocean acidification among strains of a model diatom |
title_full_unstemmed |
Physiological and molecular responses to ocean acidification among strains of a model diatom |
title_sort |
physiological and molecular responses to ocean acidification among strains of a model diatom |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1002/lno.11565 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11565 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.11565 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11565 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Limnology and Oceanography volume 65, issue 12, page 2926-2936 ISSN 0024-3590 1939-5590 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/lno.11565 |
container_title |
Limnology and Oceanography |
container_volume |
65 |
container_issue |
12 |
container_start_page |
2926 |
op_container_end_page |
2936 |
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1797591088340402176 |