Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer
Abstract Plankton are an extremely diverse and polyphyletic group, exhibiting a large range in morphological and physiological traits. Here, we apply automated optical techniques, provided by the pulse‐shape recording automated flow cytometer—CytoSense—to investigate trait variability of phytoplankt...
Published in: | Limnology and Oceanography |
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crwiley:10.1002/lno.11189 2024-06-02T08:01:30+00:00 Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer Fragoso, Glaucia Moreira Poulton, Alex James Pratt, Nicola Jane Johnsen, Geir Purdie, Duncan Alastair Conselho Nacional de Desenvolvimento Científico e Tecnológico Natural Environment Research Council Norges Forskningsråd 2019 http://dx.doi.org/10.1002/lno.11189 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11189 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.11189 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11189 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Limnology and Oceanography volume 64, issue 4, page 1763-1778 ISSN 0024-3590 1939-5590 journal-article 2019 crwiley https://doi.org/10.1002/lno.11189 2024-05-03T10:45:18Z Abstract Plankton are an extremely diverse and polyphyletic group, exhibiting a large range in morphological and physiological traits. Here, we apply automated optical techniques, provided by the pulse‐shape recording automated flow cytometer—CytoSense—to investigate trait variability of phytoplankton and plastidic ciliates in Arctic and Atlantic waters of the subpolar North Atlantic. We used the bio‐optical descriptors derived from the CytoSense (light scattering [forward and sideward] and fluorescence [red, yellow/green and orange from chlorophyll a , degraded pigments, and phycobiliproteins, respectively]) and translated them into functional traits to demonstrate ecological trait variability along an environmental gradient. Cell size was the master trait varying in this study, with large photosynthetic microplankton (> 20 μ m in cell diameter), including diatoms as single cells and chains, as well as plastidic ciliates found in Arctic waters, while small‐sized phytoplankton groups, such as the picoeukaryotes (< 4 μ m) and the cyanobacteria Synechococcus were dominant in Atlantic waters. Morphological traits, such as chain/colony formation and structural complexity (i.e., cellular processes, setae, and internal vacuoles), appear to favor buoyancy in highly illuminated and stratified Arctic waters. In Atlantic waters, small cell size and spherical cell shape, in addition to photo‐physiological traits, such as high internal pigmentation, offer chromatic adaptation for survival in the low nutrient and dynamic mixing waters of the Atlantic Ocean. The use of automated techniques that quantify ecological traits holds exciting new opportunities to unravel linkages between the structure and function of plankton communities and marine ecosystems. Article in Journal/Newspaper Arctic North Atlantic Phytoplankton Wiley Online Library Arctic Limnology and Oceanography |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Plankton are an extremely diverse and polyphyletic group, exhibiting a large range in morphological and physiological traits. Here, we apply automated optical techniques, provided by the pulse‐shape recording automated flow cytometer—CytoSense—to investigate trait variability of phytoplankton and plastidic ciliates in Arctic and Atlantic waters of the subpolar North Atlantic. We used the bio‐optical descriptors derived from the CytoSense (light scattering [forward and sideward] and fluorescence [red, yellow/green and orange from chlorophyll a , degraded pigments, and phycobiliproteins, respectively]) and translated them into functional traits to demonstrate ecological trait variability along an environmental gradient. Cell size was the master trait varying in this study, with large photosynthetic microplankton (> 20 μ m in cell diameter), including diatoms as single cells and chains, as well as plastidic ciliates found in Arctic waters, while small‐sized phytoplankton groups, such as the picoeukaryotes (< 4 μ m) and the cyanobacteria Synechococcus were dominant in Atlantic waters. Morphological traits, such as chain/colony formation and structural complexity (i.e., cellular processes, setae, and internal vacuoles), appear to favor buoyancy in highly illuminated and stratified Arctic waters. In Atlantic waters, small cell size and spherical cell shape, in addition to photo‐physiological traits, such as high internal pigmentation, offer chromatic adaptation for survival in the low nutrient and dynamic mixing waters of the Atlantic Ocean. The use of automated techniques that quantify ecological traits holds exciting new opportunities to unravel linkages between the structure and function of plankton communities and marine ecosystems. |
author2 |
Conselho Nacional de Desenvolvimento Científico e Tecnológico Natural Environment Research Council Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Fragoso, Glaucia Moreira Poulton, Alex James Pratt, Nicola Jane Johnsen, Geir Purdie, Duncan Alastair |
spellingShingle |
Fragoso, Glaucia Moreira Poulton, Alex James Pratt, Nicola Jane Johnsen, Geir Purdie, Duncan Alastair Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer |
author_facet |
Fragoso, Glaucia Moreira Poulton, Alex James Pratt, Nicola Jane Johnsen, Geir Purdie, Duncan Alastair |
author_sort |
Fragoso, Glaucia Moreira |
title |
Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer |
title_short |
Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer |
title_full |
Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer |
title_fullStr |
Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer |
title_full_unstemmed |
Trait‐based analysis of subpolar North Atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer |
title_sort |
trait‐based analysis of subpolar north atlantic phytoplankton and plastidic ciliate communities using automated flow cytometer |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/lno.11189 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11189 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.11189 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.11189 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic North Atlantic Phytoplankton |
genre_facet |
Arctic North Atlantic Phytoplankton |
op_source |
Limnology and Oceanography volume 64, issue 4, page 1763-1778 ISSN 0024-3590 1939-5590 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/lno.11189 |
container_title |
Limnology and Oceanography |
_version_ |
1800745888431210496 |