Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean)
Abstract Marine nitrogen fixation occurs not only in subtropical and tropical regions but also in colder regions. However, the distribution of diazotrophs, nitrogen fixation rate, and its contribution to the nitrogen cycle in the Arctic Ocean remain poorly understood. We examined the diazotroph comm...
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Online Access: | http://dx.doi.org/10.1002/lno.10933 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flno.10933 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.10933 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.10933 https://onlinelibrary.wiley.com/doi/am-pdf/10.1002%2Flno.10933 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.10933 |
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crwiley:10.1002/lno.10933 2024-09-15T17:53:16+00:00 Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean) Shiozaki, Takuhei Fujiwara, Amane Ijichi, Minoru Harada, Naomi Nishino, Shigeto Nishi, Shinro Nagata, Toshi Hamasaki, Koji Japan Society for the Promotion of Science 2018 http://dx.doi.org/10.1002/lno.10933 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flno.10933 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.10933 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.10933 https://onlinelibrary.wiley.com/doi/am-pdf/10.1002%2Flno.10933 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.10933 en eng Wiley http://creativecommons.org/licenses/by-nc-nd/4.0/ Limnology and Oceanography volume 63, issue 5, page 2191-2205 ISSN 0024-3590 1939-5590 journal-article 2018 crwiley https://doi.org/10.1002/lno.10933 2024-08-01T04:22:45Z Abstract Marine nitrogen fixation occurs not only in subtropical and tropical regions but also in colder regions. However, the distribution of diazotrophs, nitrogen fixation rate, and its contribution to the nitrogen cycle in the Arctic Ocean remain poorly understood. We examined the diazotroph community structure and activity in the shelf and off‐shelf regions of the Chukchi Sea, western Arctic Ocean, during late summer 2015. The nitrate and ammonium assimilation rates were determined simultaneously to gain insights into the role of nitrogen fixation in the nitrogen cycle of the region. The diazotroph community determined by Illumina sequencing was mainly composed of Cluster III nifH phylotypes (putative anaerobes), accounting for > 80% of the total sequences examined, except for one surface sample. This result is strikingly different from previous findings in other oceanic regions. The nifH sequences other than those from Cluster III were mostly affiliated with UCYN‐A2 (symbiotic cyanobacteria), which accounted for < 15% of the total sequences. UCYN‐A2 tended to be abundant (maximum 2.9 × 10 3 copies L −1 ) in the high‐temperature low‐salinity water mass that is characteristic of Pacific‐originating water. Nitrogen fixation rate was detectable at most stations, with a range of 0.08–3.60 nmol L −1 d −1 , displaying no clear relationship with depth (light intensity) or nitrate or ammonium concentration. Nitrogen fixation locally exceeded nitrate assimilation, but accounted for 1.00% at most in the total nitrogen assimilation in the euphotic zone. Article in Journal/Newspaper Arctic Ocean Chukchi Chukchi Sea Wiley Online Library Limnology and Oceanography 63 5 2191 2205 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Marine nitrogen fixation occurs not only in subtropical and tropical regions but also in colder regions. However, the distribution of diazotrophs, nitrogen fixation rate, and its contribution to the nitrogen cycle in the Arctic Ocean remain poorly understood. We examined the diazotroph community structure and activity in the shelf and off‐shelf regions of the Chukchi Sea, western Arctic Ocean, during late summer 2015. The nitrate and ammonium assimilation rates were determined simultaneously to gain insights into the role of nitrogen fixation in the nitrogen cycle of the region. The diazotroph community determined by Illumina sequencing was mainly composed of Cluster III nifH phylotypes (putative anaerobes), accounting for > 80% of the total sequences examined, except for one surface sample. This result is strikingly different from previous findings in other oceanic regions. The nifH sequences other than those from Cluster III were mostly affiliated with UCYN‐A2 (symbiotic cyanobacteria), which accounted for < 15% of the total sequences. UCYN‐A2 tended to be abundant (maximum 2.9 × 10 3 copies L −1 ) in the high‐temperature low‐salinity water mass that is characteristic of Pacific‐originating water. Nitrogen fixation rate was detectable at most stations, with a range of 0.08–3.60 nmol L −1 d −1 , displaying no clear relationship with depth (light intensity) or nitrate or ammonium concentration. Nitrogen fixation locally exceeded nitrate assimilation, but accounted for 1.00% at most in the total nitrogen assimilation in the euphotic zone. |
author2 |
Japan Society for the Promotion of Science |
format |
Article in Journal/Newspaper |
author |
Shiozaki, Takuhei Fujiwara, Amane Ijichi, Minoru Harada, Naomi Nishino, Shigeto Nishi, Shinro Nagata, Toshi Hamasaki, Koji |
spellingShingle |
Shiozaki, Takuhei Fujiwara, Amane Ijichi, Minoru Harada, Naomi Nishino, Shigeto Nishi, Shinro Nagata, Toshi Hamasaki, Koji Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean) |
author_facet |
Shiozaki, Takuhei Fujiwara, Amane Ijichi, Minoru Harada, Naomi Nishino, Shigeto Nishi, Shinro Nagata, Toshi Hamasaki, Koji |
author_sort |
Shiozaki, Takuhei |
title |
Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean) |
title_short |
Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean) |
title_full |
Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean) |
title_fullStr |
Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean) |
title_full_unstemmed |
Diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the Chukchi Sea (western Arctic Ocean) |
title_sort |
diazotroph community structure and the role of nitrogen fixation in the nitrogen cycle in the chukchi sea (western arctic ocean) |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/lno.10933 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flno.10933 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lno.10933 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lno.10933 https://onlinelibrary.wiley.com/doi/am-pdf/10.1002%2Flno.10933 https://aslopubs.onlinelibrary.wiley.com/doi/pdf/10.1002/lno.10933 |
genre |
Arctic Ocean Chukchi Chukchi Sea |
genre_facet |
Arctic Ocean Chukchi Chukchi Sea |
op_source |
Limnology and Oceanography volume 63, issue 5, page 2191-2205 ISSN 0024-3590 1939-5590 |
op_rights |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
op_doi |
https://doi.org/10.1002/lno.10933 |
container_title |
Limnology and Oceanography |
container_volume |
63 |
container_issue |
5 |
container_start_page |
2191 |
op_container_end_page |
2205 |
_version_ |
1810295298816212992 |