The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8
Abstract Wax esters (WE) belong to the class of neutral lipids. They are formed by an esterification of a fatty alcohol and an activated fatty acid. Dependent on the chain length and desaturation degree of the fatty acid and the fatty alcohol moiety, WE can have diverse physicochemical properties. W...
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crwiley:10.1002/lipd.12250 2024-06-02T08:14:53+00:00 The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8 Vollheyde, Katharina Yu, Dan Hornung, Ellen Herrfurth, Cornelia Feussner, Ivo Deutsche Forschungsgemeinschaft 2020 http://dx.doi.org/10.1002/lipd.12250 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flipd.12250 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lipd.12250 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lipd.12250 en eng Wiley http://creativecommons.org/licenses/by/4.0/ Lipids volume 55, issue 5, page 479-494 ISSN 0024-4201 1558-9307 journal-article 2020 crwiley https://doi.org/10.1002/lipd.12250 2024-05-03T10:57:56Z Abstract Wax esters (WE) belong to the class of neutral lipids. They are formed by an esterification of a fatty alcohol and an activated fatty acid. Dependent on the chain length and desaturation degree of the fatty acid and the fatty alcohol moiety, WE can have diverse physicochemical properties. WE derived from monounsaturated long‐chain acyl moieties are of industrial interest due to their very good lubrication properties. Whereas WE were obtained in the past from spermaceti organs of the sperm whale, industrial WE are nowadays mostly produced chemically from fossil fuels. In order to produce WE more sustainably, attempts to produce industrial WE in transgenic plants are steadily increasing. To achieve this, different combinations of WE producing enzymes are expressed in developing Arabidopsis thaliana or Camelina sativa seeds. Here we report the identification and characterization of a fifth wax synthase from the organism Marinobacter aquaeolei VT8, MaWSD5. It belongs to the class of bifunctional wax synthase/acyl‐CoA:diacylglycerol O ‐acyltransferases (WSD). The protein was purified to homogeneity. In vivo and in vitro substrate analyses revealed that MaWSD5 is able to synthesize WE but no triacylglycerols. The protein produces WE from saturated and monounsaturated mid‐ and long‐chain substrates. Arabidopsis thaliana seeds expressing a fatty acid reductase from Marinobacter aquaeolei VT8 and MaWSD5 produce WE. Main WE synthesized are 20:1/18:1 and 20:1/20:1. This makes MaWSD5 a suitable candidate for industrial WE production in planta . Article in Journal/Newspaper Sperm whale Wiley Online Library Lipids 55 5 479 494 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Wax esters (WE) belong to the class of neutral lipids. They are formed by an esterification of a fatty alcohol and an activated fatty acid. Dependent on the chain length and desaturation degree of the fatty acid and the fatty alcohol moiety, WE can have diverse physicochemical properties. WE derived from monounsaturated long‐chain acyl moieties are of industrial interest due to their very good lubrication properties. Whereas WE were obtained in the past from spermaceti organs of the sperm whale, industrial WE are nowadays mostly produced chemically from fossil fuels. In order to produce WE more sustainably, attempts to produce industrial WE in transgenic plants are steadily increasing. To achieve this, different combinations of WE producing enzymes are expressed in developing Arabidopsis thaliana or Camelina sativa seeds. Here we report the identification and characterization of a fifth wax synthase from the organism Marinobacter aquaeolei VT8, MaWSD5. It belongs to the class of bifunctional wax synthase/acyl‐CoA:diacylglycerol O ‐acyltransferases (WSD). The protein was purified to homogeneity. In vivo and in vitro substrate analyses revealed that MaWSD5 is able to synthesize WE but no triacylglycerols. The protein produces WE from saturated and monounsaturated mid‐ and long‐chain substrates. Arabidopsis thaliana seeds expressing a fatty acid reductase from Marinobacter aquaeolei VT8 and MaWSD5 produce WE. Main WE synthesized are 20:1/18:1 and 20:1/20:1. This makes MaWSD5 a suitable candidate for industrial WE production in planta . |
author2 |
Deutsche Forschungsgemeinschaft |
format |
Article in Journal/Newspaper |
author |
Vollheyde, Katharina Yu, Dan Hornung, Ellen Herrfurth, Cornelia Feussner, Ivo |
spellingShingle |
Vollheyde, Katharina Yu, Dan Hornung, Ellen Herrfurth, Cornelia Feussner, Ivo The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8 |
author_facet |
Vollheyde, Katharina Yu, Dan Hornung, Ellen Herrfurth, Cornelia Feussner, Ivo |
author_sort |
Vollheyde, Katharina |
title |
The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8 |
title_short |
The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8 |
title_full |
The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8 |
title_fullStr |
The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8 |
title_full_unstemmed |
The Fifth WS/DGAT Enzyme of the Bacterium Marinobacter aquaeolei VT8 |
title_sort |
fifth ws/dgat enzyme of the bacterium marinobacter aquaeolei vt8 |
publisher |
Wiley |
publishDate |
2020 |
url |
http://dx.doi.org/10.1002/lipd.12250 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flipd.12250 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lipd.12250 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lipd.12250 |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
Lipids volume 55, issue 5, page 479-494 ISSN 0024-4201 1558-9307 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/lipd.12250 |
container_title |
Lipids |
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55 |
container_issue |
5 |
container_start_page |
479 |
op_container_end_page |
494 |
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1800738900234207232 |