Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar)
Abstract Adequate dietary supply of eicosapentaenoic acid (20:5n‐3) and docosahexaenoic acid (22:6n‐3) is required to maintain health and growth of Atlantic salmon ( Salmo salar) . However, salmon can also convert α‐linolenic acid (18:3n‐3) into eicosapentaenoic acid (20:5n‐3) and docosahexaenoic ac...
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crwiley:10.1002/lipd.12198 2024-09-30T14:32:24+00:00 Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar) Irvine, Nicola A. Ruyter, Bente Østbye, Tone‐Kari Sonesson, Anna K. Lillycrop, Karen A. Berge, Gerd Burdge, Graham C. Norges Forskningsråd 2019 http://dx.doi.org/10.1002/lipd.12198 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lipd.12198 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lipd.12198 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 54, issue 11-12, page 725-739 ISSN 0024-4201 1558-9307 journal-article 2019 crwiley https://doi.org/10.1002/lipd.12198 2024-09-05T05:08:07Z Abstract Adequate dietary supply of eicosapentaenoic acid (20:5n‐3) and docosahexaenoic acid (22:6n‐3) is required to maintain health and growth of Atlantic salmon ( Salmo salar) . However, salmon can also convert α‐linolenic acid (18:3n‐3) into eicosapentaenoic acid (20:5n‐3) and docosahexaenoic acid (22:6n‐3) by sequential desaturation and elongation reactions, which can be modified by 20:5n‐3 and 22:6n‐3 intake. In mammals, dietary 20:5n‐3 + 22:6n‐3 intake can modify Fads2 expression (Δ6 desaturase) via altered DNA methylation of its promoter. Decreasing dietary fish oil (FO) has been shown to increase Δ5fad expression in salmon liver. However, it is not known whether this is associated with changes in the DNA methylation of genes involved in polyunsaturated fatty acid synthesis. To address this, we investigated whether changing the proportions of dietary FO and vegetable oil altered the DNA methylation of Δ6fad_b , Δ5fad , Elovl2, and Elovl5_b promoters in liver and muscle from Atlantic salmon and whether any changes were associated with mRNA expression. Higher dietary FO content increased the proportions of 20:5n‐3 and 22:6n‐3 and decreased Δ6fad_b mRNA expression in liver, but there was no effect on Δ5fad, Elovl2, and Elovl5_b expression. There were significant differences between liver and skeletal muscle in the methylation of individual CpG loci in all four genes studied. Methylation of individual Δ6fad_b CpG loci was negatively related to its expression and to proportions of 20:5n‐3 and 22:6n‐3 in the liver. These findings suggest variations in dietary FO can induce gene‐, CpG locus‐, and tissue‐related changes in DNA methylation in salmon. Article in Journal/Newspaper Atlantic salmon Salmo salar Wiley Online Library Lipids 54 11-12 725 739 |
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English |
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Abstract Adequate dietary supply of eicosapentaenoic acid (20:5n‐3) and docosahexaenoic acid (22:6n‐3) is required to maintain health and growth of Atlantic salmon ( Salmo salar) . However, salmon can also convert α‐linolenic acid (18:3n‐3) into eicosapentaenoic acid (20:5n‐3) and docosahexaenoic acid (22:6n‐3) by sequential desaturation and elongation reactions, which can be modified by 20:5n‐3 and 22:6n‐3 intake. In mammals, dietary 20:5n‐3 + 22:6n‐3 intake can modify Fads2 expression (Δ6 desaturase) via altered DNA methylation of its promoter. Decreasing dietary fish oil (FO) has been shown to increase Δ5fad expression in salmon liver. However, it is not known whether this is associated with changes in the DNA methylation of genes involved in polyunsaturated fatty acid synthesis. To address this, we investigated whether changing the proportions of dietary FO and vegetable oil altered the DNA methylation of Δ6fad_b , Δ5fad , Elovl2, and Elovl5_b promoters in liver and muscle from Atlantic salmon and whether any changes were associated with mRNA expression. Higher dietary FO content increased the proportions of 20:5n‐3 and 22:6n‐3 and decreased Δ6fad_b mRNA expression in liver, but there was no effect on Δ5fad, Elovl2, and Elovl5_b expression. There were significant differences between liver and skeletal muscle in the methylation of individual CpG loci in all four genes studied. Methylation of individual Δ6fad_b CpG loci was negatively related to its expression and to proportions of 20:5n‐3 and 22:6n‐3 in the liver. These findings suggest variations in dietary FO can induce gene‐, CpG locus‐, and tissue‐related changes in DNA methylation in salmon. |
author2 |
Norges Forskningsråd |
format |
Article in Journal/Newspaper |
author |
Irvine, Nicola A. Ruyter, Bente Østbye, Tone‐Kari Sonesson, Anna K. Lillycrop, Karen A. Berge, Gerd Burdge, Graham C. |
spellingShingle |
Irvine, Nicola A. Ruyter, Bente Østbye, Tone‐Kari Sonesson, Anna K. Lillycrop, Karen A. Berge, Gerd Burdge, Graham C. Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar) |
author_facet |
Irvine, Nicola A. Ruyter, Bente Østbye, Tone‐Kari Sonesson, Anna K. Lillycrop, Karen A. Berge, Gerd Burdge, Graham C. |
author_sort |
Irvine, Nicola A. |
title |
Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar) |
title_short |
Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar) |
title_full |
Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar) |
title_fullStr |
Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar) |
title_full_unstemmed |
Dietary Fish Oil Alters DNA Methylation of Genes Involved in Polyunsaturated Fatty Acid Biosynthesis in Muscle and Liver of Atlantic Salmon ( Salmo salar) |
title_sort |
dietary fish oil alters dna methylation of genes involved in polyunsaturated fatty acid biosynthesis in muscle and liver of atlantic salmon ( salmo salar) |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/lipd.12198 https://onlinelibrary.wiley.com/doi/pdf/10.1002/lipd.12198 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/lipd.12198 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Lipids volume 54, issue 11-12, page 725-739 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/lipd.12198 |
container_title |
Lipids |
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54 |
container_issue |
11-12 |
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725 |
op_container_end_page |
739 |
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1811636575330631680 |