Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes
Abstract Several feeding trials with Atlantic salmon fed naturally high phytosterol concentrations due to dietary rapeseed oil inclusion have shown changes in lipid metabolism and increased hepatic lipid storage in the fish. An in vitro trial with Atlantic salmon hepatocytes was, therefore, performe...
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Online Access: | http://dx.doi.org/10.1002/lipd.12083 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flipd.12083 http://onlinelibrary.wiley.com/wol1/doi/10.1002/lipd.12083/fullpdf |
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crwiley:10.1002/lipd.12083 2024-06-02T08:03:24+00:00 Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes Liland, Nina S. Pittman, Karin Whatmore, Paul Torstensen, Bente E. Sissener, Nini H. The Norwegian Seafood Research Fund-FHF Institute of Marine Research, Norway The Research Council of Norway 2018 http://dx.doi.org/10.1002/lipd.12083 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flipd.12083 http://onlinelibrary.wiley.com/wol1/doi/10.1002/lipd.12083/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Lipids volume 53, issue 7, page 737-747 ISSN 0024-4201 1558-9307 journal-article 2018 crwiley https://doi.org/10.1002/lipd.12083 2024-05-03T11:00:11Z Abstract Several feeding trials with Atlantic salmon fed naturally high phytosterol concentrations due to dietary rapeseed oil inclusion have shown changes in lipid metabolism and increased hepatic lipid storage in the fish. An in vitro trial with Atlantic salmon hepatocytes was, therefore, performed to study the possible direct effects of phytosterols on lipid storage and metabolism. The isolated hepatocytes were exposed to seven different sterol treatments and gene expression, as well as lipid accumulation by Oil Red O dyeing, was assessed. Fucosterol, a sterol found in many algae species, had an effect on the size of individual lipid droplets, leading to smaller lipid droplets than in the control without added sterols. A sterol extract from soybean/rapeseed led to an increase in the percentage of hepatocytes with visible lipid droplets at 20× magnification, while hepatocytes of both the sterol extract‐treated groups and fucosterol‐treated groups had a larger proportion of their area covered with lipids compared to control cells. Brassicasterol, a sterol characteristic of rapeseed oil, was the only sterol treatment leading to a change in gene expression, affecting the expression of the nuclear receptors, peroxisome proliferator‐activated receptor gamma ( pparg ) and retinoid X receptor ( rxr ). The current study thus shows that phytosterols can have direct, although subtle, effects on both hepatic lipid storage and gene expression of Atlantic salmon in vitro . Article in Journal/Newspaper Atlantic salmon Wiley Online Library Lipids 53 7 737 747 |
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Open Polar |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract Several feeding trials with Atlantic salmon fed naturally high phytosterol concentrations due to dietary rapeseed oil inclusion have shown changes in lipid metabolism and increased hepatic lipid storage in the fish. An in vitro trial with Atlantic salmon hepatocytes was, therefore, performed to study the possible direct effects of phytosterols on lipid storage and metabolism. The isolated hepatocytes were exposed to seven different sterol treatments and gene expression, as well as lipid accumulation by Oil Red O dyeing, was assessed. Fucosterol, a sterol found in many algae species, had an effect on the size of individual lipid droplets, leading to smaller lipid droplets than in the control without added sterols. A sterol extract from soybean/rapeseed led to an increase in the percentage of hepatocytes with visible lipid droplets at 20× magnification, while hepatocytes of both the sterol extract‐treated groups and fucosterol‐treated groups had a larger proportion of their area covered with lipids compared to control cells. Brassicasterol, a sterol characteristic of rapeseed oil, was the only sterol treatment leading to a change in gene expression, affecting the expression of the nuclear receptors, peroxisome proliferator‐activated receptor gamma ( pparg ) and retinoid X receptor ( rxr ). The current study thus shows that phytosterols can have direct, although subtle, effects on both hepatic lipid storage and gene expression of Atlantic salmon in vitro . |
author2 |
The Norwegian Seafood Research Fund-FHF Institute of Marine Research, Norway The Research Council of Norway |
format |
Article in Journal/Newspaper |
author |
Liland, Nina S. Pittman, Karin Whatmore, Paul Torstensen, Bente E. Sissener, Nini H. |
spellingShingle |
Liland, Nina S. Pittman, Karin Whatmore, Paul Torstensen, Bente E. Sissener, Nini H. Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes |
author_facet |
Liland, Nina S. Pittman, Karin Whatmore, Paul Torstensen, Bente E. Sissener, Nini H. |
author_sort |
Liland, Nina S. |
title |
Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes |
title_short |
Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes |
title_full |
Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes |
title_fullStr |
Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes |
title_full_unstemmed |
Fucosterol Causes Small Changes in Lipid Storage and Brassicasterol Affects some Markers of Lipid Metabolism in Atlantic Salmon Hepatocytes |
title_sort |
fucosterol causes small changes in lipid storage and brassicasterol affects some markers of lipid metabolism in atlantic salmon hepatocytes |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/lipd.12083 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Flipd.12083 http://onlinelibrary.wiley.com/wol1/doi/10.1002/lipd.12083/fullpdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Lipids volume 53, issue 7, page 737-747 ISSN 0024-4201 1558-9307 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/lipd.12083 |
container_title |
Lipids |
container_volume |
53 |
container_issue |
7 |
container_start_page |
737 |
op_container_end_page |
747 |
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1800747913098297344 |