Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana)
Abstract HPLC atmospheric pressure chemical ionization (APCI)/MS, GC MS, HPLC diode array detection (DAD), and NMR were used for the identification of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana ). Matrix solid phase dispersion was applied for the extraction of th...
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crwiley:10.1002/jssc.200500152 2024-06-23T07:52:34+00:00 Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana) Grynbaum, Marc David Hentschel, Petra Putzbach, Karsten Rehbein, Jens Krucker, Manfred Nicholson, Graeme Albert, Klaus 2005 http://dx.doi.org/10.1002/jssc.200500152 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjssc.200500152 https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.200500152 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Separation Science volume 28, issue 14, page 1685-1693 ISSN 1615-9306 1615-9314 journal-article 2005 crwiley https://doi.org/10.1002/jssc.200500152 2024-06-06T04:21:25Z Abstract HPLC atmospheric pressure chemical ionization (APCI)/MS, GC MS, HPLC diode array detection (DAD), and NMR were used for the identification of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana ). Matrix solid phase dispersion was applied for the extraction of the carotenoids. This gentle and expeditious extraction technique for solid and viscous samples leads to distinct higher enrichment rates than the conventional liquid–liquid extraction. The chromatographic separation was achieved employing a C 30 RP column that allows the separation of shape‐constrained geometrical isomers. A methanol/ tert ‐butylmethyl ether/water gradient was applied. ( all ‐E) Astaxanthin and the geometrical isomers were identified by HPLC APCI/MS, by coelution with isomerized authentical standard, by UV spectroscopy (DAD), and three isomers were unambiguously assigned by microcoil NMR spectroscopy. In this method, microcoils are transversally aligned to the magnetic field and have an increased sensitivity compared to the conventional double‐saddle Helmholtz coils, thus enabling the measurement on small samples. The carotenol fatty acid esters were saponified enzymatically with Lipase type VII from Candida rugosa . The fatty acids were detected by GC MS after transesterification, but also without previous derivatization by HPLC APCI/MS. C14:0, C16:0, C16:1, C18:1, C20:0, C20:5, and C22:6 were found in astaxanthin monoesters and in astaxanthin diesters. ( all ‐E) Astaxanthin was identified as the main isomer in six fatty acid ester fractions by NMR. Quantitation was carried out by the method of internal standard. (13‐ cis ) Astaxanthin (70 μg/g), 542 μg/g ( all ‐E) astaxanthin, 36 μg/g unidentified astaxanthin isomer, 62 μg/g (9‐ cis ) astaxanthin, and 7842 μg/g astaxanthin fatty acid esters were found. Article in Journal/Newspaper Euphausia superba Wiley Online Library Rugosa ENVELOPE(-61.250,-61.250,-62.633,-62.633) Journal of Separation Science 28 14 1685 1693 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract HPLC atmospheric pressure chemical ionization (APCI)/MS, GC MS, HPLC diode array detection (DAD), and NMR were used for the identification of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana ). Matrix solid phase dispersion was applied for the extraction of the carotenoids. This gentle and expeditious extraction technique for solid and viscous samples leads to distinct higher enrichment rates than the conventional liquid–liquid extraction. The chromatographic separation was achieved employing a C 30 RP column that allows the separation of shape‐constrained geometrical isomers. A methanol/ tert ‐butylmethyl ether/water gradient was applied. ( all ‐E) Astaxanthin and the geometrical isomers were identified by HPLC APCI/MS, by coelution with isomerized authentical standard, by UV spectroscopy (DAD), and three isomers were unambiguously assigned by microcoil NMR spectroscopy. In this method, microcoils are transversally aligned to the magnetic field and have an increased sensitivity compared to the conventional double‐saddle Helmholtz coils, thus enabling the measurement on small samples. The carotenol fatty acid esters were saponified enzymatically with Lipase type VII from Candida rugosa . The fatty acids were detected by GC MS after transesterification, but also without previous derivatization by HPLC APCI/MS. C14:0, C16:0, C16:1, C18:1, C20:0, C20:5, and C22:6 were found in astaxanthin monoesters and in astaxanthin diesters. ( all ‐E) Astaxanthin was identified as the main isomer in six fatty acid ester fractions by NMR. Quantitation was carried out by the method of internal standard. (13‐ cis ) Astaxanthin (70 μg/g), 542 μg/g ( all ‐E) astaxanthin, 36 μg/g unidentified astaxanthin isomer, 62 μg/g (9‐ cis ) astaxanthin, and 7842 μg/g astaxanthin fatty acid esters were found. |
format |
Article in Journal/Newspaper |
author |
Grynbaum, Marc David Hentschel, Petra Putzbach, Karsten Rehbein, Jens Krucker, Manfred Nicholson, Graeme Albert, Klaus |
spellingShingle |
Grynbaum, Marc David Hentschel, Petra Putzbach, Karsten Rehbein, Jens Krucker, Manfred Nicholson, Graeme Albert, Klaus Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana) |
author_facet |
Grynbaum, Marc David Hentschel, Petra Putzbach, Karsten Rehbein, Jens Krucker, Manfred Nicholson, Graeme Albert, Klaus |
author_sort |
Grynbaum, Marc David |
title |
Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana) |
title_short |
Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana) |
title_full |
Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana) |
title_fullStr |
Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana) |
title_full_unstemmed |
Unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( Euphausia superba Dana) |
title_sort |
unambiguous detection of astaxanthin and astaxanthin fatty acid esters in krill ( euphausia superba dana) |
publisher |
Wiley |
publishDate |
2005 |
url |
http://dx.doi.org/10.1002/jssc.200500152 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjssc.200500152 https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.200500152 |
long_lat |
ENVELOPE(-61.250,-61.250,-62.633,-62.633) |
geographic |
Rugosa |
geographic_facet |
Rugosa |
genre |
Euphausia superba |
genre_facet |
Euphausia superba |
op_source |
Journal of Separation Science volume 28, issue 14, page 1685-1693 ISSN 1615-9306 1615-9314 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jssc.200500152 |
container_title |
Journal of Separation Science |
container_volume |
28 |
container_issue |
14 |
container_start_page |
1685 |
op_container_end_page |
1693 |
_version_ |
1802643909987270656 |