Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes
Abstract Introduction Modic changes (MC) are bone marrow lesions of vertebral bones, which can be detected with magnetic resonance imaging (MRI) adjacent to degenerated intervertebral discs. Defined by their appearance on T1 and T2 weighted images, there are three interconvertible types: MC1, MC2, a...
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crwiley:10.1002/jsp2.1337 2024-09-15T18:25:39+00:00 Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes Schulze, Friederike Määttä, Juhani Grad, Sybille Heggli, Irina Brunner, Florian Farshad, Mazda Distler, Oliver Karppinen, Jaro Lotz, Jeffrey Dudli, Stefan National Institute of Arthritis and Musculoskeletal and Skin Diseases Balgrist Stiftung Oulun Yliopisto AO Foundation Velux Stiftung Oulun Yliopistollinen Sairaala 2024 http://dx.doi.org/10.1002/jsp2.1337 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsp2.1337 en eng Wiley http://creativecommons.org/licenses/by/4.0/ JOR SPINE volume 7, issue 3 ISSN 2572-1143 2572-1143 journal-article 2024 crwiley https://doi.org/10.1002/jsp2.1337 2024-07-18T04:27:00Z Abstract Introduction Modic changes (MC) are bone marrow lesions of vertebral bones, which can be detected with magnetic resonance imaging (MRI) adjacent to degenerated intervertebral discs. Defined by their appearance on T1 and T2 weighted images, there are three interconvertible types: MC1, MC2, and MC3. The inter‐observer variability of the MRI diagnosis is high, therefore a diagnostic serum biomarker complementing the MRI to facilitate diagnosis and follow‐up would be of great value. Methods We used a highly sensitive and reproducible proteomics approach: DIA/SWATH‐MS to find serum biomarkers in a subset of the Northern Finland Birth Cohort 1966. Separately, we measured a panel of factors involved in inflammation and angiogenesis to confirm some potential biomarkers published before with an ELISA‐based method called V‐Plex. Results We found neither an association between the serum concentrations of the proteins detected with DIA/SWATH‐MS with the presence of MC, nor a correlation with the size of the MC lesions. We did not find any association between the factors measured with the V‐Plex and the presence of MC or their size. Conclusion Altogether, our study suggests that a robust and generally usable biomarker to facilitate the diagnosis of MC cannot readily be found in serum. Article in Journal/Newspaper Northern Finland Wiley Online Library JOR SPINE 7 3 |
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description |
Abstract Introduction Modic changes (MC) are bone marrow lesions of vertebral bones, which can be detected with magnetic resonance imaging (MRI) adjacent to degenerated intervertebral discs. Defined by their appearance on T1 and T2 weighted images, there are three interconvertible types: MC1, MC2, and MC3. The inter‐observer variability of the MRI diagnosis is high, therefore a diagnostic serum biomarker complementing the MRI to facilitate diagnosis and follow‐up would be of great value. Methods We used a highly sensitive and reproducible proteomics approach: DIA/SWATH‐MS to find serum biomarkers in a subset of the Northern Finland Birth Cohort 1966. Separately, we measured a panel of factors involved in inflammation and angiogenesis to confirm some potential biomarkers published before with an ELISA‐based method called V‐Plex. Results We found neither an association between the serum concentrations of the proteins detected with DIA/SWATH‐MS with the presence of MC, nor a correlation with the size of the MC lesions. We did not find any association between the factors measured with the V‐Plex and the presence of MC or their size. Conclusion Altogether, our study suggests that a robust and generally usable biomarker to facilitate the diagnosis of MC cannot readily be found in serum. |
author2 |
National Institute of Arthritis and Musculoskeletal and Skin Diseases Balgrist Stiftung Oulun Yliopisto AO Foundation Velux Stiftung Oulun Yliopistollinen Sairaala |
format |
Article in Journal/Newspaper |
author |
Schulze, Friederike Määttä, Juhani Grad, Sybille Heggli, Irina Brunner, Florian Farshad, Mazda Distler, Oliver Karppinen, Jaro Lotz, Jeffrey Dudli, Stefan |
spellingShingle |
Schulze, Friederike Määttä, Juhani Grad, Sybille Heggli, Irina Brunner, Florian Farshad, Mazda Distler, Oliver Karppinen, Jaro Lotz, Jeffrey Dudli, Stefan Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes |
author_facet |
Schulze, Friederike Määttä, Juhani Grad, Sybille Heggli, Irina Brunner, Florian Farshad, Mazda Distler, Oliver Karppinen, Jaro Lotz, Jeffrey Dudli, Stefan |
author_sort |
Schulze, Friederike |
title |
Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes |
title_short |
Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes |
title_full |
Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes |
title_fullStr |
Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes |
title_full_unstemmed |
Proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for Modic changes |
title_sort |
proteomic analysis of serum in a population‐based cohort did not reveal a biomarker for modic changes |
publisher |
Wiley |
publishDate |
2024 |
url |
http://dx.doi.org/10.1002/jsp2.1337 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsp2.1337 |
genre |
Northern Finland |
genre_facet |
Northern Finland |
op_source |
JOR SPINE volume 7, issue 3 ISSN 2572-1143 2572-1143 |
op_rights |
http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1002/jsp2.1337 |
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JOR SPINE |
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7 |
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3 |
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1810466151662092288 |