Gene analysis and structure prediction for the cold‐adaption mechanism of trypsin from the krill Euphausia superba (Dana, 1852)

• Published in: Journal of the Science of Food and Agriculture
• Main Authors: Zhou, Tingting, Wang, Xichang, Yan, Juan, Li, Yan
• Other Authors: Shanghai Jiao Tong University
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2018
• Subjects: Antarctic; Antarc*; Antarctic Krill; Euphausia superba
• Online Access: http://dx.doi.org/10.1002/jsfa.8804; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.8804; https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.8804

Abstract BACKGROUND The ability of Antarctic krill, Euphausia superba (Dana, 1852), to thrive in a cold environment comes from its capacity to synthesize cold‐adapted enzymes. Its trypsin, as a main substance in the metabolic reactions, plays a key role in the adaption to low temperatures. However, the progress of research on its cold‐adaption mechanism is being influenced due to the limited information on its gene and spatial structure. RESULTS We studied the gene of E. superba trypsin with transcriptome sequencing first, and then discussed its cold‐adaption mechanism with the full gene and predicted structure basing on bioinformatics. The results showed the proportion of certain residues played important roles in the cold‐adaptation behavior for trypsin. Furthermore, a higher proportion of random coils and reduced steric hindrance might also be key factors promoting its cold adaption. CONCLUSION This research aimed to reveal the cold‐adaption mechanism of E. superba trypsin and provide support for basic research on molecular modification by site‐directed mutagenesis of complementary DNA used to produce new and improved recombinant variants with cold adaption. Furthermore, it may broaden its commercial application on minimizing undesirable changes elevated at higher temperature in food processing and in treatment of trauma and inflammation in medicine. © 2017 Society of Chemical Industry