The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
Abstract BACKGROUND The matrix effect is considered to be a problem in the immunoassay of foodstuffs. However, information on the interference from aquatic products, as well as the mechanism involved, is very limited. In this study, using three flatfishes ( Scophthalmus maximus , Paralichthys olivac...
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crwiley:10.1002/jsfa.5931 2024-10-06T13:52:36+00:00 The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics Wang, Xiudan Lin, Hong Sui, Jianxin Cao, Limin 2012 http://dx.doi.org/10.1002/jsfa.5931 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.5931 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.5931 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the Science of Food and Agriculture volume 93, issue 7, page 1603-1609 ISSN 0022-5142 1097-0010 journal-article 2012 crwiley https://doi.org/10.1002/jsfa.5931 2024-09-17T04:52:27Z Abstract BACKGROUND The matrix effect is considered to be a problem in the immunoassay of foodstuffs. However, information on the interference from aquatic products, as well as the mechanism involved, is very limited. In this study, using three flatfishes ( Scophthalmus maximus , Paralichthys olivaceus and Cymoglossus robustus ) as samples, the effect of the fish matrix on the competitive indirect enzyme‐linked immunosorbent assay (ci‐ ELISA ) of antibiotic (norfloxacin) residues was investigated. The mechanism of the observed matrix effect is also preliminarily discussed . RESULTS Within the working range of the calibration curves, a significant ( P = 0.05) but irregular variation in the inhibition ratio was observed in the presence of fish extracts. Further experiments revealed that such a matrix effect could be caused by some water‐soluble fish proteins with a wide range of molecular weight (from below 14.4 kDa to about 116.0 kDa ), and the ions from fish muscles may also contribute to the interference. The results of western blotting indicated that some fish protein components might effectively bind with antibody reagents used . CONCLUSION Significant interference in the immunoassay of norfloxacin was observed in the presence of fish matrix. Some proteins and ions were demonstrated to contribute to the matrix effect investigated. Although the detailed mechanism is still unclear, the non‐specific interaction between fish proteins and immunoglobulin G ( IgG ) or horseradish peroxidase (HRP) labelled IgG was assumed to be an important source of the matrix effect in immunoassays. © 2012 Society of Chemical Industry Article in Journal/Newspaper Scophthalmus maximus Wiley Online Library Journal of the Science of Food and Agriculture 93 7 1603 1609 |
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English |
description |
Abstract BACKGROUND The matrix effect is considered to be a problem in the immunoassay of foodstuffs. However, information on the interference from aquatic products, as well as the mechanism involved, is very limited. In this study, using three flatfishes ( Scophthalmus maximus , Paralichthys olivaceus and Cymoglossus robustus ) as samples, the effect of the fish matrix on the competitive indirect enzyme‐linked immunosorbent assay (ci‐ ELISA ) of antibiotic (norfloxacin) residues was investigated. The mechanism of the observed matrix effect is also preliminarily discussed . RESULTS Within the working range of the calibration curves, a significant ( P = 0.05) but irregular variation in the inhibition ratio was observed in the presence of fish extracts. Further experiments revealed that such a matrix effect could be caused by some water‐soluble fish proteins with a wide range of molecular weight (from below 14.4 kDa to about 116.0 kDa ), and the ions from fish muscles may also contribute to the interference. The results of western blotting indicated that some fish protein components might effectively bind with antibody reagents used . CONCLUSION Significant interference in the immunoassay of norfloxacin was observed in the presence of fish matrix. Some proteins and ions were demonstrated to contribute to the matrix effect investigated. Although the detailed mechanism is still unclear, the non‐specific interaction between fish proteins and immunoglobulin G ( IgG ) or horseradish peroxidase (HRP) labelled IgG was assumed to be an important source of the matrix effect in immunoassays. © 2012 Society of Chemical Industry |
format |
Article in Journal/Newspaper |
author |
Wang, Xiudan Lin, Hong Sui, Jianxin Cao, Limin |
spellingShingle |
Wang, Xiudan Lin, Hong Sui, Jianxin Cao, Limin The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics |
author_facet |
Wang, Xiudan Lin, Hong Sui, Jianxin Cao, Limin |
author_sort |
Wang, Xiudan |
title |
The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics |
title_short |
The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics |
title_full |
The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics |
title_fullStr |
The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics |
title_full_unstemmed |
The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics |
title_sort |
effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1002/jsfa.5931 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.5931 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.5931 |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_source |
Journal of the Science of Food and Agriculture volume 93, issue 7, page 1603-1609 ISSN 0022-5142 1097-0010 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jsfa.5931 |
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Journal of the Science of Food and Agriculture |
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93 |
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7 |
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1603 |
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1609 |
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1812181036327501824 |