The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics

Abstract BACKGROUND The matrix effect is considered to be a problem in the immunoassay of foodstuffs. However, information on the interference from aquatic products, as well as the mechanism involved, is very limited. In this study, using three flatfishes ( Scophthalmus maximus , Paralichthys olivac...

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Published in:Journal of the Science of Food and Agriculture
Main Authors: Wang, Xiudan, Lin, Hong, Sui, Jianxin, Cao, Limin
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2012
Subjects:
Online Access:http://dx.doi.org/10.1002/jsfa.5931
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spelling crwiley:10.1002/jsfa.5931 2024-10-06T13:52:36+00:00 The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics Wang, Xiudan Lin, Hong Sui, Jianxin Cao, Limin 2012 http://dx.doi.org/10.1002/jsfa.5931 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.5931 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.5931 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the Science of Food and Agriculture volume 93, issue 7, page 1603-1609 ISSN 0022-5142 1097-0010 journal-article 2012 crwiley https://doi.org/10.1002/jsfa.5931 2024-09-17T04:52:27Z Abstract BACKGROUND The matrix effect is considered to be a problem in the immunoassay of foodstuffs. However, information on the interference from aquatic products, as well as the mechanism involved, is very limited. In this study, using three flatfishes ( Scophthalmus maximus , Paralichthys olivaceus and Cymoglossus robustus ) as samples, the effect of the fish matrix on the competitive indirect enzyme‐linked immunosorbent assay (ci‐ ELISA ) of antibiotic (norfloxacin) residues was investigated. The mechanism of the observed matrix effect is also preliminarily discussed . RESULTS Within the working range of the calibration curves, a significant ( P = 0.05) but irregular variation in the inhibition ratio was observed in the presence of fish extracts. Further experiments revealed that such a matrix effect could be caused by some water‐soluble fish proteins with a wide range of molecular weight (from below 14.4 kDa to about 116.0 kDa ), and the ions from fish muscles may also contribute to the interference. The results of western blotting indicated that some fish protein components might effectively bind with antibody reagents used . CONCLUSION Significant interference in the immunoassay of norfloxacin was observed in the presence of fish matrix. Some proteins and ions were demonstrated to contribute to the matrix effect investigated. Although the detailed mechanism is still unclear, the non‐specific interaction between fish proteins and immunoglobulin G ( IgG ) or horseradish peroxidase (HRP) labelled IgG was assumed to be an important source of the matrix effect in immunoassays. © 2012 Society of Chemical Industry Article in Journal/Newspaper Scophthalmus maximus Wiley Online Library Journal of the Science of Food and Agriculture 93 7 1603 1609
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collection Wiley Online Library
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language English
description Abstract BACKGROUND The matrix effect is considered to be a problem in the immunoassay of foodstuffs. However, information on the interference from aquatic products, as well as the mechanism involved, is very limited. In this study, using three flatfishes ( Scophthalmus maximus , Paralichthys olivaceus and Cymoglossus robustus ) as samples, the effect of the fish matrix on the competitive indirect enzyme‐linked immunosorbent assay (ci‐ ELISA ) of antibiotic (norfloxacin) residues was investigated. The mechanism of the observed matrix effect is also preliminarily discussed . RESULTS Within the working range of the calibration curves, a significant ( P = 0.05) but irregular variation in the inhibition ratio was observed in the presence of fish extracts. Further experiments revealed that such a matrix effect could be caused by some water‐soluble fish proteins with a wide range of molecular weight (from below 14.4 kDa to about 116.0 kDa ), and the ions from fish muscles may also contribute to the interference. The results of western blotting indicated that some fish protein components might effectively bind with antibody reagents used . CONCLUSION Significant interference in the immunoassay of norfloxacin was observed in the presence of fish matrix. Some proteins and ions were demonstrated to contribute to the matrix effect investigated. Although the detailed mechanism is still unclear, the non‐specific interaction between fish proteins and immunoglobulin G ( IgG ) or horseradish peroxidase (HRP) labelled IgG was assumed to be an important source of the matrix effect in immunoassays. © 2012 Society of Chemical Industry
format Article in Journal/Newspaper
author Wang, Xiudan
Lin, Hong
Sui, Jianxin
Cao, Limin
spellingShingle Wang, Xiudan
Lin, Hong
Sui, Jianxin
Cao, Limin
The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
author_facet Wang, Xiudan
Lin, Hong
Sui, Jianxin
Cao, Limin
author_sort Wang, Xiudan
title The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
title_short The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
title_full The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
title_fullStr The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
title_full_unstemmed The effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
title_sort effect of fish matrix on the enzyme‐linked immunosorbent assay of antibiotics
publisher Wiley
publishDate 2012
url http://dx.doi.org/10.1002/jsfa.5931
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.5931
https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.5931
genre Scophthalmus maximus
genre_facet Scophthalmus maximus
op_source Journal of the Science of Food and Agriculture
volume 93, issue 7, page 1603-1609
ISSN 0022-5142 1097-0010
op_rights http://onlinelibrary.wiley.com/termsAndConditions#vor
op_doi https://doi.org/10.1002/jsfa.5931
container_title Journal of the Science of Food and Agriculture
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container_issue 7
container_start_page 1603
op_container_end_page 1609
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