A method for the determination of available fluoride from marine products using young chicks

Abstract A method is described for the determination of available fluoride in biological materials. Groups of 1‐day‐old male White Leghorn chicks were fed a low fluoride diet for 14 days (2.3 mg F − kg −1 ) to which graded levels of fluoride were added as sodium fluoride, or in the form of fluoride‐...

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Bibliographic Details
Published in:Journal of the Science of Food and Agriculture
Main Authors: Soevik, Torleif, Braekkan, Olaf R.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1981
Subjects:
Online Access:http://dx.doi.org/10.1002/jsfa.2740320507
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.2740320507
https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.2740320507
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Summary:Abstract A method is described for the determination of available fluoride in biological materials. Groups of 1‐day‐old male White Leghorn chicks were fed a low fluoride diet for 14 days (2.3 mg F − kg −1 ) to which graded levels of fluoride were added as sodium fluoride, or in the form of fluoride‐containing foods. The drinking water did not contain any detectable amount of fluoride. Water and feeds were provided ad lib . The response was measured as the concentration of fluoride in the three bones of the third toe from both feet on a fat‐free dry matter basis. A linear accumulation was observed for the addition of up to 500 mg F − kg −1 to the diet. An experimental dose range between 0 and 8 mg kg −1 was chosen. The availability of fluoride was measured as the slope ratio between highly correlated regression lines for test material and a standard. The lines had a common intersection on the y ‐axis. Relative to sodium fluoride, the biological availability of fluoride from different types of fish protein concentrate showed values of 110‐151%. The corresponding values for krill ( Meganyctiphanes norvegica ) and a fluoridated toothpaste containing sodium monofluoro‐phosphate were 112 and 145%, respectively.