Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar)
Abstract The gelatinolytic activity in muscle from Atlantic cod ( Gadus morhua ), spotted wolffish ( Anarhichas minor ) and Atlantic salmon ( Salmo salar ) was studied using gelatin SDS‐PAGE, gelatin affinity chromatography and enzyme inhibitors. These fish species are known to differ markedly in fi...
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crwiley:10.1002/jsfa.1501 2024-06-02T08:03:07+00:00 Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar) Lødemel, Jørgen B Olsen, Ragnar L 2003 http://dx.doi.org/10.1002/jsfa.1501 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.1501 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.1501 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the Science of Food and Agriculture volume 83, issue 10, page 1031-1036 ISSN 0022-5142 1097-0010 journal-article 2003 crwiley https://doi.org/10.1002/jsfa.1501 2024-05-03T11:45:26Z Abstract The gelatinolytic activity in muscle from Atlantic cod ( Gadus morhua ), spotted wolffish ( Anarhichas minor ) and Atlantic salmon ( Salmo salar ) was studied using gelatin SDS‐PAGE, gelatin affinity chromatography and enzyme inhibitors. These fish species are known to differ markedly in fillet softening and gaping post mortem . Atlantic cod, which is a promising species for cold water marine aquaculture, often shows such negative properties, particularly after being well fed. Gelatinolytic activity bands were present in all three species. Using gelatin chromatography and enzyme inhibitors, both serine proteinases and metalloproteinases were detected in wolffish and cod muscle, while only the latter were found in salmon muscle. Activation of the metalloproteinases by p ‐aminophenylmercuric acetate (APMA) resulted in a shift in activity from higher to lower molecular weight, as is known for mammalian matrix metalloproteinases. In all three species the molecular weight of the metalloproteinases was lowered from approximately 80 to about 70 kDa by activating with APMA. Copyright © 2003 Society of Chemical Industry Article in Journal/Newspaper atlantic cod Atlantic salmon Gadus morhua Salmo salar Wiley Online Library Journal of the Science of Food and Agriculture 83 10 1031 1036 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract The gelatinolytic activity in muscle from Atlantic cod ( Gadus morhua ), spotted wolffish ( Anarhichas minor ) and Atlantic salmon ( Salmo salar ) was studied using gelatin SDS‐PAGE, gelatin affinity chromatography and enzyme inhibitors. These fish species are known to differ markedly in fillet softening and gaping post mortem . Atlantic cod, which is a promising species for cold water marine aquaculture, often shows such negative properties, particularly after being well fed. Gelatinolytic activity bands were present in all three species. Using gelatin chromatography and enzyme inhibitors, both serine proteinases and metalloproteinases were detected in wolffish and cod muscle, while only the latter were found in salmon muscle. Activation of the metalloproteinases by p ‐aminophenylmercuric acetate (APMA) resulted in a shift in activity from higher to lower molecular weight, as is known for mammalian matrix metalloproteinases. In all three species the molecular weight of the metalloproteinases was lowered from approximately 80 to about 70 kDa by activating with APMA. Copyright © 2003 Society of Chemical Industry |
format |
Article in Journal/Newspaper |
author |
Lødemel, Jørgen B Olsen, Ragnar L |
spellingShingle |
Lødemel, Jørgen B Olsen, Ragnar L Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar) |
author_facet |
Lødemel, Jørgen B Olsen, Ragnar L |
author_sort |
Lødemel, Jørgen B |
title |
Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar) |
title_short |
Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar) |
title_full |
Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar) |
title_fullStr |
Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar) |
title_full_unstemmed |
Gelatinolytic activities in muscle of Atlantic cod ( Gadus morhua), spotted wolffish ( Anarhichas minor) and Atlantic salmon ( Salmo salar) |
title_sort |
gelatinolytic activities in muscle of atlantic cod ( gadus morhua), spotted wolffish ( anarhichas minor) and atlantic salmon ( salmo salar) |
publisher |
Wiley |
publishDate |
2003 |
url |
http://dx.doi.org/10.1002/jsfa.1501 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjsfa.1501 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.1501 |
genre |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
atlantic cod Atlantic salmon Gadus morhua Salmo salar |
op_source |
Journal of the Science of Food and Agriculture volume 83, issue 10, page 1031-1036 ISSN 0022-5142 1097-0010 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jsfa.1501 |
container_title |
Journal of the Science of Food and Agriculture |
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83 |
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10 |
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1031 |
op_container_end_page |
1036 |
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1800747593278423040 |