Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus)
Abstract BACKGROUND Parvalbumin (PV) can be subdivided into two phylogenetic lineages, αPV and βPV. The bony fish βPV is considered a major fish allergen. However, there is no available report on the immunological property and epitope mapping of bony fish αPV. RESULTS To characterize the allergenic...
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Online Access: | http://dx.doi.org/10.1002/jsfa.12432 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.12432 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jsfa.12432 |
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crwiley:10.1002/jsfa.12432 2024-09-15T18:34:00+00:00 Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus) Huang, Yuhao Li, Zhenxing Wu, Yeting Li, Yonghong Pramod, Siddanakoppalu Chen, Guanzhi Zhu, Wenjia Zhang, Ziye Wang, Hao Lin, Hong Fundamental Research Funds for the Central Universities 2023 http://dx.doi.org/10.1002/jsfa.12432 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.12432 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jsfa.12432 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the Science of Food and Agriculture volume 103, issue 5, page 2313-2324 ISSN 0022-5142 1097-0010 journal-article 2023 crwiley https://doi.org/10.1002/jsfa.12432 2024-09-03T04:25:16Z Abstract BACKGROUND Parvalbumin (PV) can be subdivided into two phylogenetic lineages, αPV and βPV. The bony fish βPV is considered a major fish allergen. However, there is no available report on the immunological property and epitope mapping of bony fish αPV. RESULTS To characterize the allergenic property of bony fish αPV and investigate the difference in allergenic property of bony fish αPV and βPV, turbot ( Scophthalmus maximus ) αPV and βPV were identified by mass spectrometry and were expressed in Escherichia coli system in this study. Spectra analysis and three‐dimensional (3D) modeling showed the similar structure between αPV and βPV. However, αPV exhibited lower immunoglobulin E/immunoglobulin G (IgE/IgG) binding capacity than βPV. Three identified βPV epitopes possessed higher IgE reactivity and more hydrophobic residues than three identified αPV epitopes. In addition, less similarity in sequence homology of αPV epitopes was observed with allergen sequences in database. CONCLUSION These finding expanded information on fish PV epitopes and substantiated the difference in allergenicity and epitope mapping between fish αPV and βPV, which will improve the epitope‐based detection tools of PV and diagnostic of PV induced fish allergy. © 2023 Society of Chemical Industry. Article in Journal/Newspaper Scophthalmus maximus Turbot Wiley Online Library Journal of the Science of Food and Agriculture 103 5 2313 2324 |
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Wiley Online Library |
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English |
description |
Abstract BACKGROUND Parvalbumin (PV) can be subdivided into two phylogenetic lineages, αPV and βPV. The bony fish βPV is considered a major fish allergen. However, there is no available report on the immunological property and epitope mapping of bony fish αPV. RESULTS To characterize the allergenic property of bony fish αPV and investigate the difference in allergenic property of bony fish αPV and βPV, turbot ( Scophthalmus maximus ) αPV and βPV were identified by mass spectrometry and were expressed in Escherichia coli system in this study. Spectra analysis and three‐dimensional (3D) modeling showed the similar structure between αPV and βPV. However, αPV exhibited lower immunoglobulin E/immunoglobulin G (IgE/IgG) binding capacity than βPV. Three identified βPV epitopes possessed higher IgE reactivity and more hydrophobic residues than three identified αPV epitopes. In addition, less similarity in sequence homology of αPV epitopes was observed with allergen sequences in database. CONCLUSION These finding expanded information on fish PV epitopes and substantiated the difference in allergenicity and epitope mapping between fish αPV and βPV, which will improve the epitope‐based detection tools of PV and diagnostic of PV induced fish allergy. © 2023 Society of Chemical Industry. |
author2 |
Fundamental Research Funds for the Central Universities |
format |
Article in Journal/Newspaper |
author |
Huang, Yuhao Li, Zhenxing Wu, Yeting Li, Yonghong Pramod, Siddanakoppalu Chen, Guanzhi Zhu, Wenjia Zhang, Ziye Wang, Hao Lin, Hong |
spellingShingle |
Huang, Yuhao Li, Zhenxing Wu, Yeting Li, Yonghong Pramod, Siddanakoppalu Chen, Guanzhi Zhu, Wenjia Zhang, Ziye Wang, Hao Lin, Hong Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus) |
author_facet |
Huang, Yuhao Li, Zhenxing Wu, Yeting Li, Yonghong Pramod, Siddanakoppalu Chen, Guanzhi Zhu, Wenjia Zhang, Ziye Wang, Hao Lin, Hong |
author_sort |
Huang, Yuhao |
title |
Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus) |
title_short |
Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus) |
title_full |
Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus) |
title_fullStr |
Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus) |
title_full_unstemmed |
Comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( Scophthalmus maximus) |
title_sort |
comparative analysis of allergenicity and predicted linear epitopes in α and β parvalbumin from turbot ( scophthalmus maximus) |
publisher |
Wiley |
publishDate |
2023 |
url |
http://dx.doi.org/10.1002/jsfa.12432 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.12432 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jsfa.12432 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Journal of the Science of Food and Agriculture volume 103, issue 5, page 2313-2324 ISSN 0022-5142 1097-0010 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jsfa.12432 |
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Journal of the Science of Food and Agriculture |
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103 |
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5 |
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2313 |
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2324 |
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1810475723124637696 |