Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas)
Abstract BACKGROUND Food allergy is a serious public nutritional health problem that has attracted extensive worldwide attention. Shellfish allergy is a long‐lasting disorder that has a lifelong impact on health. Sarcoplasmic calcium‐binding protein (SCP) plays a vital role in cell and muscle functi...
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crwiley:10.1002/jsfa.11699 2024-06-02T08:05:36+00:00 Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas) Cheng, Qingli Feng, Xiaowen Zhao, Xiaohan Gu, Ruizeng Lu, Jun Liu, Wenying Li, Guoming 2021 http://dx.doi.org/10.1002/jsfa.11699 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.11699 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jsfa.11699 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of the Science of Food and Agriculture volume 102, issue 9, page 3551-3562 ISSN 0022-5142 1097-0010 journal-article 2021 crwiley https://doi.org/10.1002/jsfa.11699 2024-05-03T11:52:59Z Abstract BACKGROUND Food allergy is a serious public nutritional health problem that has attracted extensive worldwide attention. Shellfish allergy is a long‐lasting disorder that has a lifelong impact on health. Sarcoplasmic calcium‐binding protein (SCP) plays a vital role in cell and muscle functions and has been identified as an allergen in oyster. RESULTS In this study, recombinant SCP (rSCP) with a molecular mass of 21 kDa was produced and identified based on SCP amino acid sequencing of Pacific oyster ( Crassostrea gigas ), and was used as a follow‐up experimental material. Its physicochemical characterization showed that purified rSCP is highly stable to heat and acid‐alkali and trypsin digestion but less resistant to pepsin digestion. We established an animal sensitization model and rSCP displayed stronger Immunoglobulin E (IgE)‐binding activity with rat serum in the rSCP + cholera toxin (CT) group compared with the CT group and a control group. Five epitope peptides were identified as linear immunodominant epitopes by indirect competitive enzyme‐linked immunosorbent assay (icELISA) for the first time. We also found that conformational epitopes may play a major role in the immunoreactivity of SCP. CONCLUSION These results are significant for understanding hypersensitization of humans to oyster and offer available preventive measures and treatment programs in further research. © 2021 Society of Chemical Industry. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Journal of the Science of Food and Agriculture |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract BACKGROUND Food allergy is a serious public nutritional health problem that has attracted extensive worldwide attention. Shellfish allergy is a long‐lasting disorder that has a lifelong impact on health. Sarcoplasmic calcium‐binding protein (SCP) plays a vital role in cell and muscle functions and has been identified as an allergen in oyster. RESULTS In this study, recombinant SCP (rSCP) with a molecular mass of 21 kDa was produced and identified based on SCP amino acid sequencing of Pacific oyster ( Crassostrea gigas ), and was used as a follow‐up experimental material. Its physicochemical characterization showed that purified rSCP is highly stable to heat and acid‐alkali and trypsin digestion but less resistant to pepsin digestion. We established an animal sensitization model and rSCP displayed stronger Immunoglobulin E (IgE)‐binding activity with rat serum in the rSCP + cholera toxin (CT) group compared with the CT group and a control group. Five epitope peptides were identified as linear immunodominant epitopes by indirect competitive enzyme‐linked immunosorbent assay (icELISA) for the first time. We also found that conformational epitopes may play a major role in the immunoreactivity of SCP. CONCLUSION These results are significant for understanding hypersensitization of humans to oyster and offer available preventive measures and treatment programs in further research. © 2021 Society of Chemical Industry. |
format |
Article in Journal/Newspaper |
author |
Cheng, Qingli Feng, Xiaowen Zhao, Xiaohan Gu, Ruizeng Lu, Jun Liu, Wenying Li, Guoming |
spellingShingle |
Cheng, Qingli Feng, Xiaowen Zhao, Xiaohan Gu, Ruizeng Lu, Jun Liu, Wenying Li, Guoming Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas) |
author_facet |
Cheng, Qingli Feng, Xiaowen Zhao, Xiaohan Gu, Ruizeng Lu, Jun Liu, Wenying Li, Guoming |
author_sort |
Cheng, Qingli |
title |
Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas) |
title_short |
Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas) |
title_full |
Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas) |
title_fullStr |
Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas) |
title_full_unstemmed |
Physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( SCP) allergen from Pacific oyster ( Crassostrea gigas) |
title_sort |
physicochemical characterization and identification of major linear epitopes of sarcoplasmic calcium‐binding protein ( scp) allergen from pacific oyster ( crassostrea gigas) |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1002/jsfa.11699 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.11699 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jsfa.11699 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal of the Science of Food and Agriculture volume 102, issue 9, page 3551-3562 ISSN 0022-5142 1097-0010 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jsfa.11699 |
container_title |
Journal of the Science of Food and Agriculture |
_version_ |
1800750453967814656 |