Encapsulation of <scp>CALB</scp> by nucleotide/metal ions coordination nanoparticles: highly selective catalysis of esterification while poor performance in glycerolysis reaction

• Published in: Journal of the Science of Food and Agriculture
• Main Authors: Chen, Wenyi, Xu, Li, Zhong, Nanjing
• Other Authors: National Natural Science Foundation of China
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2021
• Subjects: Nutrition and Dietetics; Agronomy and Crop Science; Food Science; Biotechnology; Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1002/jsfa.11516; https://onlinelibrary.wiley.com/doi/pdf/10.1002/jsfa.11516; https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jsfa.11516

Abstract BACKGROUND Enzymatic esterification is attracting for particular high‐acid oil deacidification. In this study, Candida antarctica lipase B (CALB) was encapsulated into a series of nucleotide‐hybrid metal coordination polymers (CPs), which were constructed by guanosine 5′‐monophosphate (GMP) and various metals. RESULTS We here found that, most of the present CPs encapsulated CALB (CALB@CPs) samples were highly selective for esterification while poor in glycerolysis reaction. They exhibited quite poor performance in glycerolysis, with triacylglycerols (TAGs) conversion lower than 5%, despite this considerable enzymatic hydrolysis activities were observed. However, they (most of them) showed good performance in esterification of fatty acids and glycerol for TAG synthesis. In addition, the GMP/Tb (CPs constructed by GMP and Tb 3+ ) encapsulated CALB (CALB@GMP/Tb) transformed over 98% of oleic acid into glycerides in the high‐acid oil deacidification process, and TAG content from 87 to 89% was obtained. Moreover, the CALB@GMP/Tb showed good reusability in the esterification system. CONCLUSION The present CALB@CPs samples are selective for esterification and suitable for high‐acid oils deacidification. This work provides a new system for enzymatic selectivity improvement study. © 2021 Society of Chemical Industry.