Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum
Abstract Cold‐active catalase (CAT) elicits great interest because of its vast prospective at the medical, commercial, and biotechnological levels. The study paper reports the production of cold‐active CAT by the strain Penicillium griseofulvum P29 isolated from Antarctic soil. Improved enzyme produ...
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Online Access: | http://dx.doi.org/10.1002/jobm.202100209 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.202100209 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jobm.202100209 |
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crwiley:10.1002/jobm.202100209 2024-06-02T07:57:30+00:00 Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum Krumova, Ekaterina Abrashev, Radoslav Dishliyska, Vladislava Stoyancheva, Galina Kostadinova, Nedelina Miteva‐Staleva, Jeny Spasova, Boryana Angelova, Maria Bulgarian National Science Fund 2021 http://dx.doi.org/10.1002/jobm.202100209 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.202100209 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jobm.202100209 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Basic Microbiology volume 61, issue 9, page 782-794 ISSN 0233-111X 1521-4028 journal-article 2021 crwiley https://doi.org/10.1002/jobm.202100209 2024-05-03T12:03:53Z Abstract Cold‐active catalase (CAT) elicits great interest because of its vast prospective at the medical, commercial, and biotechnological levels. The study paper reports the production of cold‐active CAT by the strain Penicillium griseofulvum P29 isolated from Antarctic soil. Improved enzyme production was achieved by optimization of medium and culture conditions. Maximum CAT was demonstrated under low glucose content (2%), 10% inoculum size, temperature 20°C, and dissolved oxygen concentration (DO) 40%. An effective laboratory technology based on changing the oxidative stress level through an increase of DO in the bioreactor was developed. The used strategy resulted in a 1.7‐ and 1.4‐fold enhanced total enzyme activity and maximum enzyme productivity. The enzyme was purified and characterized. P. griseofulvum P29 CAT was most active at approximately 20°C and pH 6.0. Its thermostability was in the range between 5°C and 40°C. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic Journal of Basic Microbiology 61 9 782 794 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Cold‐active catalase (CAT) elicits great interest because of its vast prospective at the medical, commercial, and biotechnological levels. The study paper reports the production of cold‐active CAT by the strain Penicillium griseofulvum P29 isolated from Antarctic soil. Improved enzyme production was achieved by optimization of medium and culture conditions. Maximum CAT was demonstrated under low glucose content (2%), 10% inoculum size, temperature 20°C, and dissolved oxygen concentration (DO) 40%. An effective laboratory technology based on changing the oxidative stress level through an increase of DO in the bioreactor was developed. The used strategy resulted in a 1.7‐ and 1.4‐fold enhanced total enzyme activity and maximum enzyme productivity. The enzyme was purified and characterized. P. griseofulvum P29 CAT was most active at approximately 20°C and pH 6.0. Its thermostability was in the range between 5°C and 40°C. |
author2 |
Bulgarian National Science Fund |
format |
Article in Journal/Newspaper |
author |
Krumova, Ekaterina Abrashev, Radoslav Dishliyska, Vladislava Stoyancheva, Galina Kostadinova, Nedelina Miteva‐Staleva, Jeny Spasova, Boryana Angelova, Maria |
spellingShingle |
Krumova, Ekaterina Abrashev, Radoslav Dishliyska, Vladislava Stoyancheva, Galina Kostadinova, Nedelina Miteva‐Staleva, Jeny Spasova, Boryana Angelova, Maria Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum |
author_facet |
Krumova, Ekaterina Abrashev, Radoslav Dishliyska, Vladislava Stoyancheva, Galina Kostadinova, Nedelina Miteva‐Staleva, Jeny Spasova, Boryana Angelova, Maria |
author_sort |
Krumova, Ekaterina |
title |
Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum |
title_short |
Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum |
title_full |
Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum |
title_fullStr |
Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum |
title_full_unstemmed |
Cold‐active catalase from the psychrotolerant fungus Penicillium griseofulvum |
title_sort |
cold‐active catalase from the psychrotolerant fungus penicillium griseofulvum |
publisher |
Wiley |
publishDate |
2021 |
url |
http://dx.doi.org/10.1002/jobm.202100209 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.202100209 https://onlinelibrary.wiley.com/doi/full-xml/10.1002/jobm.202100209 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Journal of Basic Microbiology volume 61, issue 9, page 782-794 ISSN 0233-111X 1521-4028 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jobm.202100209 |
container_title |
Journal of Basic Microbiology |
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61 |
container_issue |
9 |
container_start_page |
782 |
op_container_end_page |
794 |
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1800740667067990016 |