Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa )
Abstract Metallothionein (MT) is a low‐molecular‐weight protein with a high metal binding capacity and plays a key role in organism adaptation to heavy metals. In this study, a metallothionein gene was successfully cloned and sequenced from Antarctic sea‐ice yeast Rhodotorula mucilaginosa AN5. Nucle...
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crwiley:10.1002/jobm.201900240 2024-04-14T08:03:39+00:00 Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa ) Kan, Guangfeng Ju, Yun Zhou, Ying Shi, Cuijuan Qiao, Yongping Yang, Yu Wang, Ruiqi Wang, Xiaofei Science and Technology Project of Weihai Key Technologies R & D Program of Shandong Natural Scientific Research Innovation Foundation in Harbin Institute of Technology 2019 http://dx.doi.org/10.1002/jobm.201900240 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201900240 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201900240 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Basic Microbiology volume 59, issue 9, page 879-889 ISSN 0233-111X 1521-4028 Applied Microbiology and Biotechnology General Medicine journal-article 2019 crwiley https://doi.org/10.1002/jobm.201900240 2024-03-19T10:55:14Z Abstract Metallothionein (MT) is a low‐molecular‐weight protein with a high metal binding capacity and plays a key role in organism adaptation to heavy metals. In this study, a metallothionein gene was successfully cloned and sequenced from Antarctic sea‐ice yeast Rhodotorula mucilaginosa AN5. Nucleotide sequencing and analysis revealed that the gene had four exons interrupted by three introns. MTs complementary DNA (named as RmMT ) had an open reading frame of 321 bp encoding a 106 amino acid protein with a predicted molecular weight of 10.3 kDa and pI of 8.49. The number of amino acids and distribution of cysteine residues indicated that RmMT was a novel family of fungal MTs. Quantitative real‐time polymerase chain reaction analysis showed that RmMT expression was elevated under copper‐induced stress. The RmMT gene was transferred into E. coli and the RmMT expressing bacteria showed improved tolerance to copper ion and increased accumulation of heavy metals, such as Cu 2+ , Pb 2+ , Zn 2+ , Cd 2+ , and Ag + . Moreover, in vitro studies, purified recombinant RmMT demonstrated that it could be used as a good scavenger of superoxide anion, hydroxyl, and 1,1‐Diphenyl‐2‐picrylhydrazyl (DPPH) radicals. In summary, these results demonstrate that RmMT plays a key role in the tolerance and bioaccumulation of heavy metals. Article in Journal/Newspaper Antarc* Antarctic Sea ice Wiley Online Library Antarctic Journal of Basic Microbiology 59 9 879 889 |
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Wiley Online Library |
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English |
topic |
Applied Microbiology and Biotechnology General Medicine |
spellingShingle |
Applied Microbiology and Biotechnology General Medicine Kan, Guangfeng Ju, Yun Zhou, Ying Shi, Cuijuan Qiao, Yongping Yang, Yu Wang, Ruiqi Wang, Xiaofei Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa ) |
topic_facet |
Applied Microbiology and Biotechnology General Medicine |
description |
Abstract Metallothionein (MT) is a low‐molecular‐weight protein with a high metal binding capacity and plays a key role in organism adaptation to heavy metals. In this study, a metallothionein gene was successfully cloned and sequenced from Antarctic sea‐ice yeast Rhodotorula mucilaginosa AN5. Nucleotide sequencing and analysis revealed that the gene had four exons interrupted by three introns. MTs complementary DNA (named as RmMT ) had an open reading frame of 321 bp encoding a 106 amino acid protein with a predicted molecular weight of 10.3 kDa and pI of 8.49. The number of amino acids and distribution of cysteine residues indicated that RmMT was a novel family of fungal MTs. Quantitative real‐time polymerase chain reaction analysis showed that RmMT expression was elevated under copper‐induced stress. The RmMT gene was transferred into E. coli and the RmMT expressing bacteria showed improved tolerance to copper ion and increased accumulation of heavy metals, such as Cu 2+ , Pb 2+ , Zn 2+ , Cd 2+ , and Ag + . Moreover, in vitro studies, purified recombinant RmMT demonstrated that it could be used as a good scavenger of superoxide anion, hydroxyl, and 1,1‐Diphenyl‐2‐picrylhydrazyl (DPPH) radicals. In summary, these results demonstrate that RmMT plays a key role in the tolerance and bioaccumulation of heavy metals. |
author2 |
Science and Technology Project of Weihai Key Technologies R & D Program of Shandong Natural Scientific Research Innovation Foundation in Harbin Institute of Technology |
format |
Article in Journal/Newspaper |
author |
Kan, Guangfeng Ju, Yun Zhou, Ying Shi, Cuijuan Qiao, Yongping Yang, Yu Wang, Ruiqi Wang, Xiaofei |
author_facet |
Kan, Guangfeng Ju, Yun Zhou, Ying Shi, Cuijuan Qiao, Yongping Yang, Yu Wang, Ruiqi Wang, Xiaofei |
author_sort |
Kan, Guangfeng |
title |
Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa ) |
title_short |
Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa ) |
title_full |
Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa ) |
title_fullStr |
Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa ) |
title_full_unstemmed |
Cloning and functional characterization of a novel metallothionein gene in Antarctic sea‐ice yeast ( Rhodotorula mucilaginosa ) |
title_sort |
cloning and functional characterization of a novel metallothionein gene in antarctic sea‐ice yeast ( rhodotorula mucilaginosa ) |
publisher |
Wiley |
publishDate |
2019 |
url |
http://dx.doi.org/10.1002/jobm.201900240 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201900240 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201900240 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Sea ice |
genre_facet |
Antarc* Antarctic Sea ice |
op_source |
Journal of Basic Microbiology volume 59, issue 9, page 879-889 ISSN 0233-111X 1521-4028 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jobm.201900240 |
container_title |
Journal of Basic Microbiology |
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59 |
container_issue |
9 |
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879 |
op_container_end_page |
889 |
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1796299935238324224 |