Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic
Glacier ice and firn cores have ecological and biotechnological importance. The present study is aimed at characterizing bacteria in crustal ice cores from Svalbard, the Arctic. Counts of viable isolates ranged from 10 to 7000 CFU/ml (mean 803 CFU/ml) while the total bacterial numbers ranged from 7....
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crwiley:10.1002/jobm.201500298 2024-09-15T18:07:56+00:00 Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic Singh, Purnima Singh, Shiv Mohan Roy, Utpal Department of Science & Technology (DST) India 2015 http://dx.doi.org/10.1002/jobm.201500298 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201500298 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201500298 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Basic Microbiology volume 56, issue 3, page 275-285 ISSN 0233-111X 1521-4028 journal-article 2015 crwiley https://doi.org/10.1002/jobm.201500298 2024-08-06T04:16:10Z Glacier ice and firn cores have ecological and biotechnological importance. The present study is aimed at characterizing bacteria in crustal ice cores from Svalbard, the Arctic. Counts of viable isolates ranged from 10 to 7000 CFU/ml (mean 803 CFU/ml) while the total bacterial numbers ranged from 7.20 × 10 4 to 2.59 × 10 7 cells ml −1 (mean 3.12 × 10 6 cells ml −1 ). Based on 16S rDNA sequence data, the identified species belonged to seven species, namely Bacillus barbaricus , Pseudomonas orientalis , Pseudomonas oryzihabitans , Pseudomonas fluorescens , Pseudomonas syncyanea , Sphingomonas dokdonensis , and Sphingomonas phyllosphaerae , with a sequence similarity ranging between 93.5 and 99.9% with taxa present in the database. The isolates exhibited unique phenotypic properties, and three isolates (MLB‐2, MLB‐5, and MLB‐9) are novel species, yet to be described. To the best of our knowledge, this is the first report on characterization of cultured bacterial communities from Svalbard ice cores. We conclude that high lipase, protease, cellulase, amylase, and urease activities expressed by most of the isolates provide a clue to the potential industrial applications of these organisms. These microbes, producing cold‐adapted enzymes may provide an opportunity for biotechnological research. Article in Journal/Newspaper glacier Svalbard Wiley Online Library Journal of Basic Microbiology 56 3 275 285 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Glacier ice and firn cores have ecological and biotechnological importance. The present study is aimed at characterizing bacteria in crustal ice cores from Svalbard, the Arctic. Counts of viable isolates ranged from 10 to 7000 CFU/ml (mean 803 CFU/ml) while the total bacterial numbers ranged from 7.20 × 10 4 to 2.59 × 10 7 cells ml −1 (mean 3.12 × 10 6 cells ml −1 ). Based on 16S rDNA sequence data, the identified species belonged to seven species, namely Bacillus barbaricus , Pseudomonas orientalis , Pseudomonas oryzihabitans , Pseudomonas fluorescens , Pseudomonas syncyanea , Sphingomonas dokdonensis , and Sphingomonas phyllosphaerae , with a sequence similarity ranging between 93.5 and 99.9% with taxa present in the database. The isolates exhibited unique phenotypic properties, and three isolates (MLB‐2, MLB‐5, and MLB‐9) are novel species, yet to be described. To the best of our knowledge, this is the first report on characterization of cultured bacterial communities from Svalbard ice cores. We conclude that high lipase, protease, cellulase, amylase, and urease activities expressed by most of the isolates provide a clue to the potential industrial applications of these organisms. These microbes, producing cold‐adapted enzymes may provide an opportunity for biotechnological research. |
author2 |
Department of Science & Technology (DST) India |
format |
Article in Journal/Newspaper |
author |
Singh, Purnima Singh, Shiv Mohan Roy, Utpal |
spellingShingle |
Singh, Purnima Singh, Shiv Mohan Roy, Utpal Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic |
author_facet |
Singh, Purnima Singh, Shiv Mohan Roy, Utpal |
author_sort |
Singh, Purnima |
title |
Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic |
title_short |
Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic |
title_full |
Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic |
title_fullStr |
Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic |
title_full_unstemmed |
Taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the High Arctic |
title_sort |
taxonomic characterization and the bio‐potential of bacteria isolated from glacier ice cores in the high arctic |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1002/jobm.201500298 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201500298 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201500298 |
genre |
glacier Svalbard |
genre_facet |
glacier Svalbard |
op_source |
Journal of Basic Microbiology volume 56, issue 3, page 275-285 ISSN 0233-111X 1521-4028 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jobm.201500298 |
container_title |
Journal of Basic Microbiology |
container_volume |
56 |
container_issue |
3 |
container_start_page |
275 |
op_container_end_page |
285 |
_version_ |
1810445302170124288 |