Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic
A yeast producing a cold‐adapted phytase was isolated from Antarctic deep‐sea sediment and identified as a Rhodotorula mucilaginosa strain JMUY14 of basidiomycetous yeasts. It was cultured in fermentation optimized by a response surface methodology based on the Box–Behnken design. The maximum activi...
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crwiley:10.1002/jobm.201400865 2024-06-02T07:57:08+00:00 Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic Yu, Peng Wang, Xue‐Ting Liu, Jing‐Wen Chinese Public Science and Technology Research Funds Projects of Ocean 2015 http://dx.doi.org/10.1002/jobm.201400865 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201400865 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201400865 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Basic Microbiology volume 55, issue 8, page 1029-1039 ISSN 0233-111X 1521-4028 journal-article 2015 crwiley https://doi.org/10.1002/jobm.201400865 2024-05-03T10:43:30Z A yeast producing a cold‐adapted phytase was isolated from Antarctic deep‐sea sediment and identified as a Rhodotorula mucilaginosa strain JMUY14 of basidiomycetous yeasts. It was cultured in fermentation optimized by a response surface methodology based on the Box–Behnken design. The maximum activity of phytase reached 205.447 U ml −1 , which was close to the predicted value of 201.948 U ml −1 and approximately 3.4 times higher than its initial activity. The extracellular phytase was purified by 15.2‐fold to homogeneity with a specific activity of 31,635 U mg −1 by (NH 4 ) 2 SO 4 precipitation, and a combination of DEAE Sepharose Fast Flow, SP Sepharose Fast Flow, and Sephadex G‐100. The molecular weight of the purified enzyme was estimated to be 63 kDa and its pI was 4.33. Its optimal temperature and pH were 50 °C and 5.0, respectively. Its activity was 85% at 37 °C, and showed good stability at pH 3.0∼7.0. When compared with mesophilic counterparts, the phytase not only exhibited a higher activity during 20∼30 °C but also had a low K m (247 µM) and high k cat (1394 s −1 ). The phytase activity was slightly stimulated in the presence of Mg 2+ , Fe 2+ , Fe 3+ , K + , Na + , Ca 2+ , EDTA, and EGTA and moderately inhibited by Cu 2+ , Zn 2+ , Mn 2+ , Ag + , PMSF, SDS, and phenylgloxal hydrate. It was resistant to both pepsin and trypsin. Since the phytase produced by the R. mucilaginosa JMUY14 showed a high specific activity, good pH stability, strong protease resistance, and high activity at low temperature, it has great potential for feed applications, especially in aquaculture. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic Journal of Basic Microbiology 55 8 1029 1039 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
A yeast producing a cold‐adapted phytase was isolated from Antarctic deep‐sea sediment and identified as a Rhodotorula mucilaginosa strain JMUY14 of basidiomycetous yeasts. It was cultured in fermentation optimized by a response surface methodology based on the Box–Behnken design. The maximum activity of phytase reached 205.447 U ml −1 , which was close to the predicted value of 201.948 U ml −1 and approximately 3.4 times higher than its initial activity. The extracellular phytase was purified by 15.2‐fold to homogeneity with a specific activity of 31,635 U mg −1 by (NH 4 ) 2 SO 4 precipitation, and a combination of DEAE Sepharose Fast Flow, SP Sepharose Fast Flow, and Sephadex G‐100. The molecular weight of the purified enzyme was estimated to be 63 kDa and its pI was 4.33. Its optimal temperature and pH were 50 °C and 5.0, respectively. Its activity was 85% at 37 °C, and showed good stability at pH 3.0∼7.0. When compared with mesophilic counterparts, the phytase not only exhibited a higher activity during 20∼30 °C but also had a low K m (247 µM) and high k cat (1394 s −1 ). The phytase activity was slightly stimulated in the presence of Mg 2+ , Fe 2+ , Fe 3+ , K + , Na + , Ca 2+ , EDTA, and EGTA and moderately inhibited by Cu 2+ , Zn 2+ , Mn 2+ , Ag + , PMSF, SDS, and phenylgloxal hydrate. It was resistant to both pepsin and trypsin. Since the phytase produced by the R. mucilaginosa JMUY14 showed a high specific activity, good pH stability, strong protease resistance, and high activity at low temperature, it has great potential for feed applications, especially in aquaculture. |
author2 |
Chinese Public Science and Technology Research Funds Projects of Ocean |
format |
Article in Journal/Newspaper |
author |
Yu, Peng Wang, Xue‐Ting Liu, Jing‐Wen |
spellingShingle |
Yu, Peng Wang, Xue‐Ting Liu, Jing‐Wen Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic |
author_facet |
Yu, Peng Wang, Xue‐Ting Liu, Jing‐Wen |
author_sort |
Yu, Peng |
title |
Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic |
title_short |
Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic |
title_full |
Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic |
title_fullStr |
Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic |
title_full_unstemmed |
Purification and characterization of a novel cold‐adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic |
title_sort |
purification and characterization of a novel cold‐adapted phytase from rhodotorula mucilaginosa strain jmuy14 isolated from antarctic |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1002/jobm.201400865 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201400865 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201400865 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Journal of Basic Microbiology volume 55, issue 8, page 1029-1039 ISSN 0233-111X 1521-4028 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jobm.201400865 |
container_title |
Journal of Basic Microbiology |
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55 |
container_issue |
8 |
container_start_page |
1029 |
op_container_end_page |
1039 |
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1800738948082827264 |