Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands
Humic substances (HS), an important fraction of soil organic carbon, are distributed widely throughout cold environments. A total of cold‐adapted 122 bacterial strains were isolated from 66 Alaska grassland soil samples based on their ability to grow on humic acids (HA), a main fraction of HS, as a...
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crwiley:10.1002/jobm.201300087 2024-06-02T08:15:02+00:00 Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands Park, Ha Ju Kim, Dockyu 2013 http://dx.doi.org/10.1002/jobm.201300087 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201300087 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201300087 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Basic Microbiology volume 55, issue 1, page 54-61 ISSN 0233-111X 1521-4028 journal-article 2013 crwiley https://doi.org/10.1002/jobm.201300087 2024-05-03T11:29:50Z Humic substances (HS), an important fraction of soil organic carbon, are distributed widely throughout cold environments. A total of cold‐adapted 122 bacterial strains were isolated from 66 Alaska grassland soil samples based on their ability to grow on humic acids (HA), a main fraction of HS, as a carbon and energy source. These isolates were identified based on 16S rRNA gene sequencing, with class Bacilli (79.5%) and γ‐Proteobacteria (17.1%) comprising the largest groups. Among them, 45 strains, mainly Paenibacillus (27 strains) and Pseudomonas (15 strains), were selected for further screening. Two strains ( Pseudomonas sp. PAMC 26793 and Paenibacillus sp. PAMC 26794) most efficiently degraded HA, but showed significant differences in their ability to grow on various monocyclic aromatics, which are putative degradative metabolites of HS. Fourier transform infrared spectra also showed substantial but different changes in HA chemical structure after incubation with each strain. Gel permeation chromatography demonstrated that depolymerization and polymerization of HA occurred during HS degradation by these newly isolated microbes. Article in Journal/Newspaper Subarctic Alaska Wiley Online Library Journal of Basic Microbiology 55 1 54 61 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Humic substances (HS), an important fraction of soil organic carbon, are distributed widely throughout cold environments. A total of cold‐adapted 122 bacterial strains were isolated from 66 Alaska grassland soil samples based on their ability to grow on humic acids (HA), a main fraction of HS, as a carbon and energy source. These isolates were identified based on 16S rRNA gene sequencing, with class Bacilli (79.5%) and γ‐Proteobacteria (17.1%) comprising the largest groups. Among them, 45 strains, mainly Paenibacillus (27 strains) and Pseudomonas (15 strains), were selected for further screening. Two strains ( Pseudomonas sp. PAMC 26793 and Paenibacillus sp. PAMC 26794) most efficiently degraded HA, but showed significant differences in their ability to grow on various monocyclic aromatics, which are putative degradative metabolites of HS. Fourier transform infrared spectra also showed substantial but different changes in HA chemical structure after incubation with each strain. Gel permeation chromatography demonstrated that depolymerization and polymerization of HA occurred during HS degradation by these newly isolated microbes. |
format |
Article in Journal/Newspaper |
author |
Park, Ha Ju Kim, Dockyu |
spellingShingle |
Park, Ha Ju Kim, Dockyu Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands |
author_facet |
Park, Ha Ju Kim, Dockyu |
author_sort |
Park, Ha Ju |
title |
Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands |
title_short |
Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands |
title_full |
Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands |
title_fullStr |
Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands |
title_full_unstemmed |
Isolation and characterization of humic substances‐degrading bacteria from the subarctic Alaska grasslands |
title_sort |
isolation and characterization of humic substances‐degrading bacteria from the subarctic alaska grasslands |
publisher |
Wiley |
publishDate |
2013 |
url |
http://dx.doi.org/10.1002/jobm.201300087 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201300087 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201300087 |
genre |
Subarctic Alaska |
genre_facet |
Subarctic Alaska |
op_source |
Journal of Basic Microbiology volume 55, issue 1, page 54-61 ISSN 0233-111X 1521-4028 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jobm.201300087 |
container_title |
Journal of Basic Microbiology |
container_volume |
55 |
container_issue |
1 |
container_start_page |
54 |
op_container_end_page |
61 |
_version_ |
1800739087277096960 |