Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China
Abstract Thermus strains are regarded as models to investigate the mechanism of thermostability of thermophiles, and phages from Thermus are particularly interesting because of their way to regulate gene expression. In this research, a Thermus bacteriophage named TSP4 ( Thermus Siphoviridae phage) w...
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crwiley:10.1002/jobm.201000116 2024-06-23T07:54:18+00:00 Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China Lin, Lianbing Hong, Wei Ji, Xiuling Han, Jian Huang, Li Wei, Yunlin 2010 http://dx.doi.org/10.1002/jobm.201000116 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201000116 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201000116 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Basic Microbiology volume 50, issue 5, page 452-456 ISSN 0233-111X 1521-4028 journal-article 2010 crwiley https://doi.org/10.1002/jobm.201000116 2024-06-13T04:24:46Z Abstract Thermus strains are regarded as models to investigate the mechanism of thermostability of thermophiles, and phages from Thermus are particularly interesting because of their way to regulate gene expression. In this research, a Thermus bacteriophage named TSP4 ( Thermus Siphoviridae phage) was isolated from Tengchong hot springs in China, and characteristics of morphology, temperature for phage production, pH and organic solvent sensitivity, DNA restriction endonuclease digestion and protein composition of TSP4 were further studied. TSP4 belonged to the Siphoviridae family and had a hexagonal head of 73 nm in diameter, an extremely long and flexible tail of 785 nm in length and 10 nm in width. TSP4 was very stable at 65 °C and pH 7.6. The capsid was apparently devoid of lipid. By SDS‐PAGE, six protein bands were found in purified virions. Despite their exceptional habitats separated by thousands of kilometers, the characteristics of this thermophilic phage showed high similarity to Thermus siphoviruses P23‐45 and P74‐26 isolated from Kamchatka peninsula hot springs in the Far East, Russia. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) Article in Journal/Newspaper Kamchatka Kamchatka Peninsula Wiley Online Library Kamchatka Peninsula ENVELOPE(160.000,160.000,56.000,56.000) Journal of Basic Microbiology 50 5 452 456 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Thermus strains are regarded as models to investigate the mechanism of thermostability of thermophiles, and phages from Thermus are particularly interesting because of their way to regulate gene expression. In this research, a Thermus bacteriophage named TSP4 ( Thermus Siphoviridae phage) was isolated from Tengchong hot springs in China, and characteristics of morphology, temperature for phage production, pH and organic solvent sensitivity, DNA restriction endonuclease digestion and protein composition of TSP4 were further studied. TSP4 belonged to the Siphoviridae family and had a hexagonal head of 73 nm in diameter, an extremely long and flexible tail of 785 nm in length and 10 nm in width. TSP4 was very stable at 65 °C and pH 7.6. The capsid was apparently devoid of lipid. By SDS‐PAGE, six protein bands were found in purified virions. Despite their exceptional habitats separated by thousands of kilometers, the characteristics of this thermophilic phage showed high similarity to Thermus siphoviruses P23‐45 and P74‐26 isolated from Kamchatka peninsula hot springs in the Far East, Russia. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
format |
Article in Journal/Newspaper |
author |
Lin, Lianbing Hong, Wei Ji, Xiuling Han, Jian Huang, Li Wei, Yunlin |
spellingShingle |
Lin, Lianbing Hong, Wei Ji, Xiuling Han, Jian Huang, Li Wei, Yunlin Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China |
author_facet |
Lin, Lianbing Hong, Wei Ji, Xiuling Han, Jian Huang, Li Wei, Yunlin |
author_sort |
Lin, Lianbing |
title |
Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China |
title_short |
Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China |
title_full |
Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China |
title_fullStr |
Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China |
title_full_unstemmed |
Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China |
title_sort |
isolation and characterization of an extremely long tail thermus bacteriophage from tengchong hot springs in china |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1002/jobm.201000116 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.201000116 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.201000116 |
long_lat |
ENVELOPE(160.000,160.000,56.000,56.000) |
geographic |
Kamchatka Peninsula |
geographic_facet |
Kamchatka Peninsula |
genre |
Kamchatka Kamchatka Peninsula |
genre_facet |
Kamchatka Kamchatka Peninsula |
op_source |
Journal of Basic Microbiology volume 50, issue 5, page 452-456 ISSN 0233-111X 1521-4028 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jobm.201000116 |
container_title |
Journal of Basic Microbiology |
container_volume |
50 |
container_issue |
5 |
container_start_page |
452 |
op_container_end_page |
456 |
_version_ |
1802646417288724480 |