Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W
Abstract A chemically defined minimal medium has been developed for growing the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W, a model system for studying cold adaptation. This natural isolate from Antarctica has an absolute requirement for two branched chain amino acids, isoleucine a...
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crwiley:10.1002/jobm.200700185 2024-09-15T17:41:14+00:00 Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W Sahu, Bhubanananda Ray, Malay K. 2008 http://dx.doi.org/10.1002/jobm.200700185 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.200700185 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.200700185 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Basic Microbiology volume 48, issue 1, page 38-47 ISSN 0233-111X 1521-4028 journal-article 2008 crwiley https://doi.org/10.1002/jobm.200700185 2024-08-06T04:21:15Z Abstract A chemically defined minimal medium has been developed for growing the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W, a model system for studying cold adaptation. This natural isolate from Antarctica has an absolute requirement for two branched chain amino acids, isoleucine and valine, in addition to low osmolality of the growth medium. The bacterium contains threonine deaminase but lacks acetohydroxyacid synthase suggesting that a defect lies in the isoleucine and valine biosynthetic pathway causing auxotrophy. Succinate was found to be preferred carbon source over glucose as it could suppress the glucose metabolizing enzymes in the cells, like in other pseudomonads. The development of the minimal medium (MM Lz ) for growing the Antarctic P. syringae Lz4W strain would be useful for investigation of the catabolite repression control mechanism at a very low temperature (below 5 °C), which is predominant in vast area of our global ecosystems. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) Article in Journal/Newspaper Antarc* Antarctic Antarctica Wiley Online Library Journal of Basic Microbiology 48 1 38 47 |
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Wiley Online Library |
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language |
English |
description |
Abstract A chemically defined minimal medium has been developed for growing the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W, a model system for studying cold adaptation. This natural isolate from Antarctica has an absolute requirement for two branched chain amino acids, isoleucine and valine, in addition to low osmolality of the growth medium. The bacterium contains threonine deaminase but lacks acetohydroxyacid synthase suggesting that a defect lies in the isoleucine and valine biosynthetic pathway causing auxotrophy. Succinate was found to be preferred carbon source over glucose as it could suppress the glucose metabolizing enzymes in the cells, like in other pseudomonads. The development of the minimal medium (MM Lz ) for growing the Antarctic P. syringae Lz4W strain would be useful for investigation of the catabolite repression control mechanism at a very low temperature (below 5 °C), which is predominant in vast area of our global ecosystems. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) |
format |
Article in Journal/Newspaper |
author |
Sahu, Bhubanananda Ray, Malay K. |
spellingShingle |
Sahu, Bhubanananda Ray, Malay K. Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W |
author_facet |
Sahu, Bhubanananda Ray, Malay K. |
author_sort |
Sahu, Bhubanananda |
title |
Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W |
title_short |
Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W |
title_full |
Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W |
title_fullStr |
Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W |
title_full_unstemmed |
Auxotrophy in natural isolate: minimal requirements for growth of the Antarctic psychrotrophic bacterium Pseudomonas syringae Lz4W |
title_sort |
auxotrophy in natural isolate: minimal requirements for growth of the antarctic psychrotrophic bacterium pseudomonas syringae lz4w |
publisher |
Wiley |
publishDate |
2008 |
url |
http://dx.doi.org/10.1002/jobm.200700185 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjobm.200700185 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jobm.200700185 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Journal of Basic Microbiology volume 48, issue 1, page 38-47 ISSN 0233-111X 1521-4028 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jobm.200700185 |
container_title |
Journal of Basic Microbiology |
container_volume |
48 |
container_issue |
1 |
container_start_page |
38 |
op_container_end_page |
47 |
_version_ |
1810487385618644992 |