Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003
Abstract The molecular epidemiology of hepatitis A virus (HAV) strains circulating in the St. Petersburg and Karelia regions was studied during 1997–2003. Hepatitis A virus RNA was isolated from both clinical samples (stools or sera) and environmental samples (sewage water). RT‐PCR was carried out u...
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crwiley:10.1002/jmv.20843 2024-06-02T08:09:46+00:00 Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003 Davidkin, Irja Zheleznova, Nina Jokinen, Sari Gorchakova, Olga Broman, Mia Mukomolov, Sergey 2007 http://dx.doi.org/10.1002/jmv.20843 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjmv.20843 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmv.20843 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Medical Virology volume 79, issue 6, page 657-662 ISSN 0146-6615 1096-9071 journal-article 2007 crwiley https://doi.org/10.1002/jmv.20843 2024-05-03T10:40:53Z Abstract The molecular epidemiology of hepatitis A virus (HAV) strains circulating in the St. Petersburg and Karelia regions was studied during 1997–2003. Hepatitis A virus RNA was isolated from both clinical samples (stools or sera) and environmental samples (sewage water). RT‐PCR was carried out using different primer pairs from the VP1/2A and VP1 genomic regions, the variable parts of the HAV genome. PCR products were sequenced and 306 nucleotides from the VP1/2A and 332 nucleotides from the VP1 region were used for phylogenetic analysis. The results show that the IA subtype was the most common during the follow‐up period: >90% of the isolated HAV strains belonged to that subtype. The HAV strains found in intravenous drug users belonged to subtypes IA and IIIA. Only one out of a total of 88 sequenced strains was of the IB subtype. The subtypes IB and IIIA were found only in 2001–2003, which suggests that new strains were introduced into the endemic situation. The results indicate the usefulness of molecular epidemiological methods in studying changes in the circulating HAV strains and in tracing transmission routes. J. Med. Virol. 79: 657–662, 2007. © 2007 Wiley‐Liss, Inc. Article in Journal/Newspaper karelia* Wiley Online Library Journal of Medical Virology 79 6 657 662 |
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Wiley Online Library |
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English |
description |
Abstract The molecular epidemiology of hepatitis A virus (HAV) strains circulating in the St. Petersburg and Karelia regions was studied during 1997–2003. Hepatitis A virus RNA was isolated from both clinical samples (stools or sera) and environmental samples (sewage water). RT‐PCR was carried out using different primer pairs from the VP1/2A and VP1 genomic regions, the variable parts of the HAV genome. PCR products were sequenced and 306 nucleotides from the VP1/2A and 332 nucleotides from the VP1 region were used for phylogenetic analysis. The results show that the IA subtype was the most common during the follow‐up period: >90% of the isolated HAV strains belonged to that subtype. The HAV strains found in intravenous drug users belonged to subtypes IA and IIIA. Only one out of a total of 88 sequenced strains was of the IB subtype. The subtypes IB and IIIA were found only in 2001–2003, which suggests that new strains were introduced into the endemic situation. The results indicate the usefulness of molecular epidemiological methods in studying changes in the circulating HAV strains and in tracing transmission routes. J. Med. Virol. 79: 657–662, 2007. © 2007 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Davidkin, Irja Zheleznova, Nina Jokinen, Sari Gorchakova, Olga Broman, Mia Mukomolov, Sergey |
spellingShingle |
Davidkin, Irja Zheleznova, Nina Jokinen, Sari Gorchakova, Olga Broman, Mia Mukomolov, Sergey Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003 |
author_facet |
Davidkin, Irja Zheleznova, Nina Jokinen, Sari Gorchakova, Olga Broman, Mia Mukomolov, Sergey |
author_sort |
Davidkin, Irja |
title |
Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003 |
title_short |
Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003 |
title_full |
Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003 |
title_fullStr |
Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003 |
title_full_unstemmed |
Molecular epidemiology of hepatitis A in St. Petersburg, Russia, 1997–2003 |
title_sort |
molecular epidemiology of hepatitis a in st. petersburg, russia, 1997–2003 |
publisher |
Wiley |
publishDate |
2007 |
url |
http://dx.doi.org/10.1002/jmv.20843 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjmv.20843 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jmv.20843 |
genre |
karelia* |
genre_facet |
karelia* |
op_source |
Journal of Medical Virology volume 79, issue 6, page 657-662 ISSN 0146-6615 1096-9071 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jmv.20843 |
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Journal of Medical Virology |
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79 |
container_issue |
6 |
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657 |
op_container_end_page |
662 |
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1800755552654983168 |