Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling
Piscirickettsia salmonis is a pathogenic bacteria known as the aetiological agent of the salmonid rickettsial syndrome and causes a high mortality in farmed salmonid fishes. Detection of P . salmonis in farmed fishes is based mainly on molecular biology and immunohistochemistry techniques. These tec...
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crwiley:10.1002/jms.3734 2024-06-09T07:49:20+00:00 Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling Olate, Verónica R. Nachtigall, Fabiane M. Santos, Leonardo S. Soto, Alex Araya, Macarena Oyanedel, Sandra Díaz, Verónica Marchant, Vanessa Rios‐Momberg, Mauricio Fondo Nacional de Desarrollo Científico y Tecnológico 2016 http://dx.doi.org/10.1002/jms.3734 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjms.3734 https://onlinelibrary.wiley.com/doi/full/10.1002/jms.3734 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Mass Spectrometry volume 51, issue 3, page 200-206 ISSN 1076-5174 1096-9888 journal-article 2016 crwiley https://doi.org/10.1002/jms.3734 2024-05-16T14:26:37Z Piscirickettsia salmonis is a pathogenic bacteria known as the aetiological agent of the salmonid rickettsial syndrome and causes a high mortality in farmed salmonid fishes. Detection of P . salmonis in farmed fishes is based mainly on molecular biology and immunohistochemistry techniques. These techniques are in most of the cases expensive and time consuming. In the search of new alternatives to detect the presence of P . salmonis in salmonid fishes, this work proposed the use of MALDI‐TOF‐MS to compare serum protein profiles from Salmo salar fish, including experimentally infected and non‐infected fishes using principal component analysis (PCA). Samples were obtained from a controlled bioassay where S. salar was challenged with P . salmonis in a cohabitation model and classified according to the presence or absence of the bacteria by real time PCR analysis. MALDI spectra of the fish serum samples showed differences in its serum protein composition. These differences were corroborated with PCA analysis. The results demonstrated that the use of both MALDI‐TOF‐MS and PCA represents a useful tool to discriminate the fish status through the analysis of salmonid serum samples. Copyright © 2016 John Wiley & Sons, Ltd. Article in Journal/Newspaper Salmo salar Wiley Online Library Journal of Mass Spectrometry 51 3 200 206 |
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English |
description |
Piscirickettsia salmonis is a pathogenic bacteria known as the aetiological agent of the salmonid rickettsial syndrome and causes a high mortality in farmed salmonid fishes. Detection of P . salmonis in farmed fishes is based mainly on molecular biology and immunohistochemistry techniques. These techniques are in most of the cases expensive and time consuming. In the search of new alternatives to detect the presence of P . salmonis in salmonid fishes, this work proposed the use of MALDI‐TOF‐MS to compare serum protein profiles from Salmo salar fish, including experimentally infected and non‐infected fishes using principal component analysis (PCA). Samples were obtained from a controlled bioassay where S. salar was challenged with P . salmonis in a cohabitation model and classified according to the presence or absence of the bacteria by real time PCR analysis. MALDI spectra of the fish serum samples showed differences in its serum protein composition. These differences were corroborated with PCA analysis. The results demonstrated that the use of both MALDI‐TOF‐MS and PCA represents a useful tool to discriminate the fish status through the analysis of salmonid serum samples. Copyright © 2016 John Wiley & Sons, Ltd. |
author2 |
Fondo Nacional de Desarrollo Científico y Tecnológico |
format |
Article in Journal/Newspaper |
author |
Olate, Verónica R. Nachtigall, Fabiane M. Santos, Leonardo S. Soto, Alex Araya, Macarena Oyanedel, Sandra Díaz, Verónica Marchant, Vanessa Rios‐Momberg, Mauricio |
spellingShingle |
Olate, Verónica R. Nachtigall, Fabiane M. Santos, Leonardo S. Soto, Alex Araya, Macarena Oyanedel, Sandra Díaz, Verónica Marchant, Vanessa Rios‐Momberg, Mauricio Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling |
author_facet |
Olate, Verónica R. Nachtigall, Fabiane M. Santos, Leonardo S. Soto, Alex Araya, Macarena Oyanedel, Sandra Díaz, Verónica Marchant, Vanessa Rios‐Momberg, Mauricio |
author_sort |
Olate, Verónica R. |
title |
Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling |
title_short |
Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling |
title_full |
Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling |
title_fullStr |
Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling |
title_full_unstemmed |
Fast detection of Piscirickettsia salmonis in Salmo salar serum through MALDI‐TOF‐MS profiling |
title_sort |
fast detection of piscirickettsia salmonis in salmo salar serum through maldi‐tof‐ms profiling |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1002/jms.3734 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjms.3734 https://onlinelibrary.wiley.com/doi/full/10.1002/jms.3734 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Journal of Mass Spectrometry volume 51, issue 3, page 200-206 ISSN 1076-5174 1096-9888 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jms.3734 |
container_title |
Journal of Mass Spectrometry |
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51 |
container_issue |
3 |
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200 |
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206 |
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1801381798925565952 |