Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry
Bacteria belonging to the Burkholderia cepacia complex (Bcc) are significant pathogens in Cystic Fibrosis (CF) patients and are resistant to a plethora of antibiotics. In this context, microorganisms from Antarctica are interesting because they produce antimicrobial compounds inhibiting the growth o...
Published in: | Journal of Mass Spectrometry |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2011
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/jms.1987 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjms.1987 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jms.1987/fullpdf |
id |
crwiley:10.1002/jms.1987 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/jms.1987 2024-09-15T17:46:17+00:00 Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry Romoli, Riccardo Papaleo, Maria Cristiana de Pascale, Donatella Tutino, Maria Luisa Michaud, Luigi LoGiudice, Angelina Fani, Renato Bartolucci, Gianluca 2011 http://dx.doi.org/10.1002/jms.1987 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjms.1987 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jms.1987/fullpdf en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Mass Spectrometry volume 46, issue 10, page 1051-1059 ISSN 1076-5174 1096-9888 journal-article 2011 crwiley https://doi.org/10.1002/jms.1987 2024-08-09T04:26:47Z Bacteria belonging to the Burkholderia cepacia complex (Bcc) are significant pathogens in Cystic Fibrosis (CF) patients and are resistant to a plethora of antibiotics. In this context, microorganisms from Antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. This is particularly true for bacteria isolated from Antarctic sponges. The aim of this work was to characterize a set of Antarctic bacteria for their ability to produce new natural drugs that could be exploited in the control of infections in CF patients by Bcc bacteria. Hence, 11 bacterial strains allocated to different genera (e.g., Pseudoalteromonas, Arthrobacter and Psychrobacter ) were tested for their ability to inhibit the growth of 21 Bcc strains and some other human pathogens. All these bacteria completely inhibited the growth of most, if not all, Bcc strains, suggesting a highly specific activity toward Bcc strains. Experimental evidences showed that the antimicrobial compounds are small volatile organic compounds, and are constitutively produced via an unknown pathway. The microbial volatile profile was obtained by SPME‐GC‐MS within the m/z interval of 40–450. Solid phase micro extraction technique affords the possibility to extract the volatile compounds in head space with a minimal sample perturbation. Principal component analysis and successive cluster discriminant analysis was applied to evaluate the relationships among the volatile organic compounds with the aim of classifying the microorganisms by their volatile profile. These data highlight the potentiality of Antarctic bacteria as novel sources of antibacterial substances to face Bcc infections in CF patients. Copyright © 2011 John Wiley & Sons, Ltd. Article in Journal/Newspaper Antarc* Antarctic Antarctica Wiley Online Library Journal of Mass Spectrometry 46 10 1051 1059 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Bacteria belonging to the Burkholderia cepacia complex (Bcc) are significant pathogens in Cystic Fibrosis (CF) patients and are resistant to a plethora of antibiotics. In this context, microorganisms from Antarctica are interesting because they produce antimicrobial compounds inhibiting the growth of other bacteria. This is particularly true for bacteria isolated from Antarctic sponges. The aim of this work was to characterize a set of Antarctic bacteria for their ability to produce new natural drugs that could be exploited in the control of infections in CF patients by Bcc bacteria. Hence, 11 bacterial strains allocated to different genera (e.g., Pseudoalteromonas, Arthrobacter and Psychrobacter ) were tested for their ability to inhibit the growth of 21 Bcc strains and some other human pathogens. All these bacteria completely inhibited the growth of most, if not all, Bcc strains, suggesting a highly specific activity toward Bcc strains. Experimental evidences showed that the antimicrobial compounds are small volatile organic compounds, and are constitutively produced via an unknown pathway. The microbial volatile profile was obtained by SPME‐GC‐MS within the m/z interval of 40–450. Solid phase micro extraction technique affords the possibility to extract the volatile compounds in head space with a minimal sample perturbation. Principal component analysis and successive cluster discriminant analysis was applied to evaluate the relationships among the volatile organic compounds with the aim of classifying the microorganisms by their volatile profile. These data highlight the potentiality of Antarctic bacteria as novel sources of antibacterial substances to face Bcc infections in CF patients. Copyright © 2011 John Wiley & Sons, Ltd. |
format |
Article in Journal/Newspaper |
author |
Romoli, Riccardo Papaleo, Maria Cristiana de Pascale, Donatella Tutino, Maria Luisa Michaud, Luigi LoGiudice, Angelina Fani, Renato Bartolucci, Gianluca |
spellingShingle |
Romoli, Riccardo Papaleo, Maria Cristiana de Pascale, Donatella Tutino, Maria Luisa Michaud, Luigi LoGiudice, Angelina Fani, Renato Bartolucci, Gianluca Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry |
author_facet |
Romoli, Riccardo Papaleo, Maria Cristiana de Pascale, Donatella Tutino, Maria Luisa Michaud, Luigi LoGiudice, Angelina Fani, Renato Bartolucci, Gianluca |
author_sort |
Romoli, Riccardo |
title |
Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry |
title_short |
Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry |
title_full |
Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry |
title_fullStr |
Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry |
title_full_unstemmed |
Characterization of the volatile profile of Antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry |
title_sort |
characterization of the volatile profile of antarctic bacteria by using solid‐phase microextraction‐gas chromatography‐mass spectrometry |
publisher |
Wiley |
publishDate |
2011 |
url |
http://dx.doi.org/10.1002/jms.1987 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjms.1987 http://onlinelibrary.wiley.com/wol1/doi/10.1002/jms.1987/fullpdf |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Journal of Mass Spectrometry volume 46, issue 10, page 1051-1059 ISSN 1076-5174 1096-9888 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jms.1987 |
container_title |
Journal of Mass Spectrometry |
container_volume |
46 |
container_issue |
10 |
container_start_page |
1051 |
op_container_end_page |
1059 |
_version_ |
1810494297189908480 |