Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii
Abstract Vitellogenin is the yolk protein precursor. Multiple vitellogenins identified in several teleosts have been attributed different roles in the control of egg buoyancy and in early embryonic vs. late larval nutrition. In this study, the cDNA encoding VtgAa was characterized in the Antarctic f...
Published in: | Journal of Experimental Zoology Part B: Molecular and Developmental Evolution |
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crwiley:10.1002/jez.b.21365 2024-06-02T07:56:14+00:00 Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii Barucca, Marco Biscotti, Maria Assunta Forconi, Mariko Regoli, Francesco Olmo, Ettore Canapa, Adriana 2010 http://dx.doi.org/10.1002/jez.b.21365 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.b.21365 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.b.21365 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Experimental Zoology Part B: Molecular and Developmental Evolution volume 314B, issue 8, page 645-652 ISSN 1552-5007 1552-5015 journal-article 2010 crwiley https://doi.org/10.1002/jez.b.21365 2024-05-03T11:49:23Z Abstract Vitellogenin is the yolk protein precursor. Multiple vitellogenins identified in several teleosts have been attributed different roles in the control of egg buoyancy and in early embryonic vs. late larval nutrition. In this study, the cDNA encoding VtgAa was characterized in the Antarctic fish Trematomus bernacchii (suborder Notothenioidei). The sequence contains 4,964 nucleotides and encodes 1,629 amino acids of the precursor molecule. To gain insights into the evolution of vitellogenin in Antarctic fishes, we identified the partial sequence of vtgAb , and vtgAa and vtgAb partial sequences of five other notothenioids. The phylogenetic analysis highlighted a close correlation between the Vtg amino acid sequences of the six Antarctic species and VtgAa and VtgAb of other perciforms. Finally, analysis of the ratio of vtgAa to vtgAb expression, evaluated in T. bernacchii by real‐time PCR, showed a considerably greater expression of vtgAa in different periods of austral summer. J. Exp. Zool. (Mol. Dev. Evol.) 314B:645–652, 2010 . © 2010 Wiley‐Liss, Inc. Article in Journal/Newspaper Antarc* Antarctic Wiley Online Library Antarctic Austral The Antarctic Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 314B 8 645 652 |
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Open Polar |
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Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Vitellogenin is the yolk protein precursor. Multiple vitellogenins identified in several teleosts have been attributed different roles in the control of egg buoyancy and in early embryonic vs. late larval nutrition. In this study, the cDNA encoding VtgAa was characterized in the Antarctic fish Trematomus bernacchii (suborder Notothenioidei). The sequence contains 4,964 nucleotides and encodes 1,629 amino acids of the precursor molecule. To gain insights into the evolution of vitellogenin in Antarctic fishes, we identified the partial sequence of vtgAb , and vtgAa and vtgAb partial sequences of five other notothenioids. The phylogenetic analysis highlighted a close correlation between the Vtg amino acid sequences of the six Antarctic species and VtgAa and VtgAb of other perciforms. Finally, analysis of the ratio of vtgAa to vtgAb expression, evaluated in T. bernacchii by real‐time PCR, showed a considerably greater expression of vtgAa in different periods of austral summer. J. Exp. Zool. (Mol. Dev. Evol.) 314B:645–652, 2010 . © 2010 Wiley‐Liss, Inc. |
format |
Article in Journal/Newspaper |
author |
Barucca, Marco Biscotti, Maria Assunta Forconi, Mariko Regoli, Francesco Olmo, Ettore Canapa, Adriana |
spellingShingle |
Barucca, Marco Biscotti, Maria Assunta Forconi, Mariko Regoli, Francesco Olmo, Ettore Canapa, Adriana Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii |
author_facet |
Barucca, Marco Biscotti, Maria Assunta Forconi, Mariko Regoli, Francesco Olmo, Ettore Canapa, Adriana |
author_sort |
Barucca, Marco |
title |
Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii |
title_short |
Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii |
title_full |
Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii |
title_fullStr |
Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii |
title_full_unstemmed |
Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii |
title_sort |
characterization and phylogenetic analysis of vitellogenin coding sequences in the antarctic fish trematomus bernacchii |
publisher |
Wiley |
publishDate |
2010 |
url |
http://dx.doi.org/10.1002/jez.b.21365 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.b.21365 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.b.21365 |
geographic |
Antarctic Austral The Antarctic |
geographic_facet |
Antarctic Austral The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution volume 314B, issue 8, page 645-652 ISSN 1552-5007 1552-5015 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jez.b.21365 |
container_title |
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution |
container_volume |
314B |
container_issue |
8 |
container_start_page |
645 |
op_container_end_page |
652 |
_version_ |
1800754434347630592 |