Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica
ABSTRACT Sex steroid hormones are widely detected in molluscs and play important roles in sex determination, gonadal tissue maturation, and gametogenesis. Nevertheless, the signaling pathways of sex steroids in cephalopod have not yet been clearly elucidated. In the present study, a full‐length sequ...
Published in: | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
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crwiley:10.1002/jez.2011 2024-09-09T19:37:30+00:00 Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica Lü, Zhen‐Ming Liu, Wan Liu, Li‐Qin Wang, Tian‐Ming Shi, Hui‐Lai Ping, Hong‐Ling Chi, Chang‐Feng Yang, Jing‐Wen Wu, Chang‐Wen Zhejiang Provincial Natural Science Foundation Foundation of National Engineering Research Center for Marine Aquaculture; 2016 http://dx.doi.org/10.1002/jez.2011 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.2011 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.2011 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Experimental Zoology Part A: Ecological Genetics and Physiology volume 325, issue 3, page 181-193 ISSN 1932-5223 1932-5231 journal-article 2016 crwiley https://doi.org/10.1002/jez.2011 2024-08-13T04:14:13Z ABSTRACT Sex steroid hormones are widely detected in molluscs and play important roles in sex determination, gonadal tissue maturation, and gametogenesis. Nevertheless, the signaling pathways of sex steroids in cephalopod have not yet been clearly elucidated. In the present study, a full‐length sequence encoding the estrogen receptor (ER) was isolated from common Chinese cuttlefish, Sepiella japonica . The sjER cDNA clone was found to contain 1,788 nucleotides including a 1,470 bp open reading frame encoding 489 amino acid (aa) residues. The deduced ER protein consisted of six nuclear receptor characteristic domains. Based on a phylogenetic analysis, the ER DNA‐binding domain and ligand‐binding domain are highly conserved compared to other mollusc ERs. Highest aa identities were found for sjER with common octopus ( Octopus vulgaris ) ER (89%) and pacific oyster ( Crassostrea gigas ) ER (61%). Tissue expression analysis confirmed that sjER was widely distributed among tissues and predominantly expressed in the brain, liver, gonad (testis and ovary), and other accessory sexual gland (nidamental gland). The ER expression was temporally upregulated in the brain, liver, and ovary during the early sexual maturation period in S. japonica , which is coincident with the fluctuation of ovary estradiol content. These suggest that sjER may be involved in regulating the reproductive cycle of S. japonica . A fusion protein transient transfections assay showed that sjER was mainly located in the nucleus, suggesting a possible orthodox working mechanism of S. japonica ER in the nucleus through a ligand‐dependent activation of specific gene transcription. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Wiley Online Library Pacific Journal of Experimental Zoology Part A: Ecological Genetics and Physiology 325 3 181 193 |
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Wiley Online Library |
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crwiley |
language |
English |
description |
ABSTRACT Sex steroid hormones are widely detected in molluscs and play important roles in sex determination, gonadal tissue maturation, and gametogenesis. Nevertheless, the signaling pathways of sex steroids in cephalopod have not yet been clearly elucidated. In the present study, a full‐length sequence encoding the estrogen receptor (ER) was isolated from common Chinese cuttlefish, Sepiella japonica . The sjER cDNA clone was found to contain 1,788 nucleotides including a 1,470 bp open reading frame encoding 489 amino acid (aa) residues. The deduced ER protein consisted of six nuclear receptor characteristic domains. Based on a phylogenetic analysis, the ER DNA‐binding domain and ligand‐binding domain are highly conserved compared to other mollusc ERs. Highest aa identities were found for sjER with common octopus ( Octopus vulgaris ) ER (89%) and pacific oyster ( Crassostrea gigas ) ER (61%). Tissue expression analysis confirmed that sjER was widely distributed among tissues and predominantly expressed in the brain, liver, gonad (testis and ovary), and other accessory sexual gland (nidamental gland). The ER expression was temporally upregulated in the brain, liver, and ovary during the early sexual maturation period in S. japonica , which is coincident with the fluctuation of ovary estradiol content. These suggest that sjER may be involved in regulating the reproductive cycle of S. japonica . A fusion protein transient transfections assay showed that sjER was mainly located in the nucleus, suggesting a possible orthodox working mechanism of S. japonica ER in the nucleus through a ligand‐dependent activation of specific gene transcription. |
author2 |
Zhejiang Provincial Natural Science Foundation Foundation of National Engineering Research Center for Marine Aquaculture; |
format |
Article in Journal/Newspaper |
author |
Lü, Zhen‐Ming Liu, Wan Liu, Li‐Qin Wang, Tian‐Ming Shi, Hui‐Lai Ping, Hong‐Ling Chi, Chang‐Feng Yang, Jing‐Wen Wu, Chang‐Wen |
spellingShingle |
Lü, Zhen‐Ming Liu, Wan Liu, Li‐Qin Wang, Tian‐Ming Shi, Hui‐Lai Ping, Hong‐Ling Chi, Chang‐Feng Yang, Jing‐Wen Wu, Chang‐Wen Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica |
author_facet |
Lü, Zhen‐Ming Liu, Wan Liu, Li‐Qin Wang, Tian‐Ming Shi, Hui‐Lai Ping, Hong‐Ling Chi, Chang‐Feng Yang, Jing‐Wen Wu, Chang‐Wen |
author_sort |
Lü, Zhen‐Ming |
title |
Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica |
title_short |
Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica |
title_full |
Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica |
title_fullStr |
Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica |
title_full_unstemmed |
Cloning, Characterization, and Expression Profile of Estrogen Receptor in Common Chinese Cuttlefish, Sepiella japonica |
title_sort |
cloning, characterization, and expression profile of estrogen receptor in common chinese cuttlefish, sepiella japonica |
publisher |
Wiley |
publishDate |
2016 |
url |
http://dx.doi.org/10.1002/jez.2011 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.2011 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.2011 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology volume 325, issue 3, page 181-193 ISSN 1932-5223 1932-5231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jez.2011 |
container_title |
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
container_volume |
325 |
container_issue |
3 |
container_start_page |
181 |
op_container_end_page |
193 |
_version_ |
1809906655628886016 |