Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry
ABSTRACT The objectives of our study were to adapt a method to measure mitochondrial function in intact mitochondria from the small crustacean Daphnia pulex and to validate if this method was sensitive enough to characterize mitochondrial metabolism in clones of the pulex complex differing in ploidy...
Published in: | Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
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crwiley:10.1002/jez.1913 2024-06-23T07:52:07+00:00 Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry Kake‐Guena, Sandrine A. Touisse, Kamal Vergilino, Roland Dufresne, France Blier, Pierre U. Lemieux, Hélène Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada 2015 http://dx.doi.org/10.1002/jez.1913 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1913 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1913 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Experimental Zoology Part A: Ecological Genetics and Physiology volume 323, issue 5, page 292-300 ISSN 1932-5223 1932-5231 journal-article 2015 crwiley https://doi.org/10.1002/jez.1913 2024-06-11T04:50:17Z ABSTRACT The objectives of our study were to adapt a method to measure mitochondrial function in intact mitochondria from the small crustacean Daphnia pulex and to validate if this method was sensitive enough to characterize mitochondrial metabolism in clones of the pulex complex differing in ploidy levels, mitochondrial DNA haplotypes, and geographic origins. Daphnia clones belonging to the Daphnia pulex complex represent a powerful model to delineate the link between mitochondrial DNA evolution and mitochondrial phenotypes, as single genotypes with divergent mtDNA can be grown under various experimental conditions. Our study included two diploid clones from temperate environments and two triploid clones from subarctic environments. The whole animal permeabilization and measurement of respiration with high‐resolution respirometry enabled the measurement of the functional capacity of specific mitochondrial complexes in four clones. When expressing the activity as ratios, our method detected significant interclonal variations. In the triploid subarctic clone from Kuujjurapik, a higher proportion of the maximal physiological oxidative phosphorylation (OXPHOS) capacity of mitochondria was supported by complex II, and a lower proportion by complex I. The triploid subarctic clone from Churchill (Manitoba) showed the lowest proportion of the maximal OXPHOS supported by complex II. Additional studies are required to determine if these differences in mitochondrial functions are related to differences in mitochondrial haplotypes or ploidy level and if they might be associated with fitness divergences and therefore selective value. J. Exp. Zool. 323A: 292–300, 2015 . © 2015 Wiley Periodicals, Inc. Article in Journal/Newspaper Churchill Subarctic Wiley Online Library Journal of Experimental Zoology Part A: Ecological Genetics and Physiology 323 5 292 300 |
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Wiley Online Library |
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English |
description |
ABSTRACT The objectives of our study were to adapt a method to measure mitochondrial function in intact mitochondria from the small crustacean Daphnia pulex and to validate if this method was sensitive enough to characterize mitochondrial metabolism in clones of the pulex complex differing in ploidy levels, mitochondrial DNA haplotypes, and geographic origins. Daphnia clones belonging to the Daphnia pulex complex represent a powerful model to delineate the link between mitochondrial DNA evolution and mitochondrial phenotypes, as single genotypes with divergent mtDNA can be grown under various experimental conditions. Our study included two diploid clones from temperate environments and two triploid clones from subarctic environments. The whole animal permeabilization and measurement of respiration with high‐resolution respirometry enabled the measurement of the functional capacity of specific mitochondrial complexes in four clones. When expressing the activity as ratios, our method detected significant interclonal variations. In the triploid subarctic clone from Kuujjurapik, a higher proportion of the maximal physiological oxidative phosphorylation (OXPHOS) capacity of mitochondria was supported by complex II, and a lower proportion by complex I. The triploid subarctic clone from Churchill (Manitoba) showed the lowest proportion of the maximal OXPHOS supported by complex II. Additional studies are required to determine if these differences in mitochondrial functions are related to differences in mitochondrial haplotypes or ploidy level and if they might be associated with fitness divergences and therefore selective value. J. Exp. Zool. 323A: 292–300, 2015 . © 2015 Wiley Periodicals, Inc. |
author2 |
Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
Kake‐Guena, Sandrine A. Touisse, Kamal Vergilino, Roland Dufresne, France Blier, Pierre U. Lemieux, Hélène |
spellingShingle |
Kake‐Guena, Sandrine A. Touisse, Kamal Vergilino, Roland Dufresne, France Blier, Pierre U. Lemieux, Hélène Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry |
author_facet |
Kake‐Guena, Sandrine A. Touisse, Kamal Vergilino, Roland Dufresne, France Blier, Pierre U. Lemieux, Hélène |
author_sort |
Kake‐Guena, Sandrine A. |
title |
Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry |
title_short |
Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry |
title_full |
Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry |
title_fullStr |
Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry |
title_full_unstemmed |
Assessment of mitochondrial functions in Daphnia pulexclones using high‐resolution respirometry |
title_sort |
assessment of mitochondrial functions in daphnia pulexclones using high‐resolution respirometry |
publisher |
Wiley |
publishDate |
2015 |
url |
http://dx.doi.org/10.1002/jez.1913 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1913 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1913 |
genre |
Churchill Subarctic |
genre_facet |
Churchill Subarctic |
op_source |
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology volume 323, issue 5, page 292-300 ISSN 1932-5223 1932-5231 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jez.1913 |
container_title |
Journal of Experimental Zoology Part A: Ecological Genetics and Physiology |
container_volume |
323 |
container_issue |
5 |
container_start_page |
292 |
op_container_end_page |
300 |
_version_ |
1802643336904835072 |