Time‐ and temperature‐dependent appearance of vitellogenin in Golgi vesicles and serum after estrogen treatment of salmon ( Salmo salar )

• Published in: Journal of Experimental Zoology
• Main Authors: Olin, T., Westman, A., von der Decken, A.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1989
• Subjects: Animal Science and Zoology; General Medicine; Salmo salar
• Online Access: http://dx.doi.org/10.1002/jez.1402490214; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1402490214; https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1402490214

Abstract The temperature‐dependent time course of vitellogenesis was examined in presmolt salmon ( Salmo salar ). Fishes were kept at 8°C or 16°C and injected with 17‐β‐estradiol. The rise in translation activity in liver preceeded that of RNA. At both acclimation temperatures, vitellogenin appeared in the Golgi vesicles at 3 days after the initial hormone injection. At 8°C, vitellogenin was detectable in the serum on day 7 and at 16°C on day 3 after the initial hormone treatment. At 8°C, protein synthesis per g wet weight of liver as well as vitellogenin content in the Golgi vesicles and in serum continued to rise during the 2 weeks of treatment. At 16°C, protein synthesis per g wet weight of liver reached a plateau during the second week of hormone treatment. A similar trend was seen in the vitellogenin content in the Golgi vesicles and the serum, suggesting a limiting step in either the translation or the export process of the protein at elevated temperature. The results indicate a correlation between translation activity, accumulation of vitellogenin in the Golgi vesicles, and concentration of vitellogenin in the blood.