Comparison of antifreeze glycopeptides from arctic and antarctic fishes
Abstract Antifreeze glycopeptides from the Atlantic tomcod Microgadus tomcod ), saffron cod ( Eleginus gracilis ), and Greenland cod ( Gadus ogac ) were isolated by ion exchange chromatography. Polyacrylamide gel electrophoresis revealed that fewer sizes of antifreeze glycopeptides are present in th...
Published in: | Journal of Experimental Zoology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
1982
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1002/jez.1402240207 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1402240207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1402240207 |
id |
crwiley:10.1002/jez.1402240207 |
---|---|
record_format |
openpolar |
spelling |
crwiley:10.1002/jez.1402240207 2024-09-15T17:41:08+00:00 Comparison of antifreeze glycopeptides from arctic and antarctic fishes O'Grady, Scott M. Schrag, Joseph D. Raymond, James A. Devries, Arthur L. 1982 http://dx.doi.org/10.1002/jez.1402240207 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1402240207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1402240207 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Experimental Zoology volume 224, issue 2, page 177-185 ISSN 0022-104X 1097-010X journal-article 1982 crwiley https://doi.org/10.1002/jez.1402240207 2024-08-06T04:18:42Z Abstract Antifreeze glycopeptides from the Atlantic tomcod Microgadus tomcod ), saffron cod ( Eleginus gracilis ), and Greenland cod ( Gadus ogac ) were isolated by ion exchange chromatography. Polyacrylamide gel electrophoresis revealed that fewer sizes of antifreeze glycopeptides are present in the serum of M. tomcod and E. gracilis compared to the Antarctic nototheniid ( Dissostichus mawsoni ), Glycopeptide 6 from M. tomcod and E. gracilis as well as glycopeptide 8 from G. ogac were sequenced and compared with known sequences of similar‐sized glycopeptides from Antarctic fishes. It was found that arginine residues in glycopeptide 6 from E. gracilis and M. tomcod occur at positions 15 and 18, that proline periodically replaces alanine (ala) at positions 7, 13, and 19. In contrast, none of the antifreeze glycopeptides from Antarctic fishes contain arginine. Threonine (thr) occupies positions 15 and 18 instead. Glycopeptide 8 from G. ogac was found to have the same sequence as glycopeptide 8 from the polar cod ( Boreogadus saida ). The results indicate that the repeating sequence (ala‐ala‐thr) appears to be highly conserved within polar fishes. However, greater variation in antifreeze glycopeptide structure exists within one family of northern cods (Gadidae) than within species belonging to four separate families of Antarctic fishes. Article in Journal/Newspaper Antarc* Antarctic Boreogadus saida Gadus ogac Greenland Greenland cod polar cod Wiley Online Library Journal of Experimental Zoology 224 2 177 185 |
institution |
Open Polar |
collection |
Wiley Online Library |
op_collection_id |
crwiley |
language |
English |
description |
Abstract Antifreeze glycopeptides from the Atlantic tomcod Microgadus tomcod ), saffron cod ( Eleginus gracilis ), and Greenland cod ( Gadus ogac ) were isolated by ion exchange chromatography. Polyacrylamide gel electrophoresis revealed that fewer sizes of antifreeze glycopeptides are present in the serum of M. tomcod and E. gracilis compared to the Antarctic nototheniid ( Dissostichus mawsoni ), Glycopeptide 6 from M. tomcod and E. gracilis as well as glycopeptide 8 from G. ogac were sequenced and compared with known sequences of similar‐sized glycopeptides from Antarctic fishes. It was found that arginine residues in glycopeptide 6 from E. gracilis and M. tomcod occur at positions 15 and 18, that proline periodically replaces alanine (ala) at positions 7, 13, and 19. In contrast, none of the antifreeze glycopeptides from Antarctic fishes contain arginine. Threonine (thr) occupies positions 15 and 18 instead. Glycopeptide 8 from G. ogac was found to have the same sequence as glycopeptide 8 from the polar cod ( Boreogadus saida ). The results indicate that the repeating sequence (ala‐ala‐thr) appears to be highly conserved within polar fishes. However, greater variation in antifreeze glycopeptide structure exists within one family of northern cods (Gadidae) than within species belonging to four separate families of Antarctic fishes. |
format |
Article in Journal/Newspaper |
author |
O'Grady, Scott M. Schrag, Joseph D. Raymond, James A. Devries, Arthur L. |
spellingShingle |
O'Grady, Scott M. Schrag, Joseph D. Raymond, James A. Devries, Arthur L. Comparison of antifreeze glycopeptides from arctic and antarctic fishes |
author_facet |
O'Grady, Scott M. Schrag, Joseph D. Raymond, James A. Devries, Arthur L. |
author_sort |
O'Grady, Scott M. |
title |
Comparison of antifreeze glycopeptides from arctic and antarctic fishes |
title_short |
Comparison of antifreeze glycopeptides from arctic and antarctic fishes |
title_full |
Comparison of antifreeze glycopeptides from arctic and antarctic fishes |
title_fullStr |
Comparison of antifreeze glycopeptides from arctic and antarctic fishes |
title_full_unstemmed |
Comparison of antifreeze glycopeptides from arctic and antarctic fishes |
title_sort |
comparison of antifreeze glycopeptides from arctic and antarctic fishes |
publisher |
Wiley |
publishDate |
1982 |
url |
http://dx.doi.org/10.1002/jez.1402240207 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1402240207 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1402240207 |
genre |
Antarc* Antarctic Boreogadus saida Gadus ogac Greenland Greenland cod polar cod |
genre_facet |
Antarc* Antarctic Boreogadus saida Gadus ogac Greenland Greenland cod polar cod |
op_source |
Journal of Experimental Zoology volume 224, issue 2, page 177-185 ISSN 0022-104X 1097-010X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jez.1402240207 |
container_title |
Journal of Experimental Zoology |
container_volume |
224 |
container_issue |
2 |
container_start_page |
177 |
op_container_end_page |
185 |
_version_ |
1810487228551397376 |