Cleavage initiation activities of microtubules and in vitro reassembled tubulins of sperm flagella
Abstract Cleavage of fish ( Oryzias latipes ) eggs was induced by injection of heterologous (sea urchin Hemicentrotus pulcherrimus and oyster Crassostrea gigas ) sperm microtubules. Cleavage initiating (CI) activity of microtubules was higher in 3% PVP suspension than in 6% BSA, and not affected sig...
| Published in: | Journal of Experimental Zoology |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
1976
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1002/jez.1401950109 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1401950109 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1401950109 |
| Summary: | Abstract Cleavage of fish ( Oryzias latipes ) eggs was induced by injection of heterologous (sea urchin Hemicentrotus pulcherrimus and oyster Crassostrea gigas ) sperm microtubules. Cleavage initiating (CI) activity of microtubules was higher in 3% PVP suspension than in 6% BSA, and not affected significantly by the concentration of microtubules themselves. The CI activity of microtubules suspended in 3% PVP was comparatively stable in the frozen state. Heat‐treatment at more than 55°C resulted in the loss of most or all of their CI activity. Such activity was observed in side‐by‐side aggregates of tubulin linear polymers of sea urchin ( Hemicentrotus pulcherrimus ) spermatozoa but not in dispersed linear polymers or tubulin dimers. Microtubules with CI activity seem to participate in initiating cleavage as astral centers, or a “seed” for polymerization of ooplasmic tubulins in activated eggs. |
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