Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas)
Abstract By gel exclusion chromatography the molecular weight of T. arcticus LDH was estimated to be 135,000–140,000 and that of MDH to be 65,000–70,000. Employing electrophoretic mobility in starch gel, molecular hybridization, and immunoprecipitation, the LDH isozymes could be classified into thre...
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crwiley:10.1002/jez.1401860206 2024-06-02T08:15:15+00:00 Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas) Massaro, Edward J. 1973 http://dx.doi.org/10.1002/jez.1401860206 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1401860206 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1401860206 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Experimental Zoology volume 186, issue 2, page 151-157 ISSN 0022-104X 1097-010X journal-article 1973 crwiley https://doi.org/10.1002/jez.1401860206 2024-05-03T10:47:29Z Abstract By gel exclusion chromatography the molecular weight of T. arcticus LDH was estimated to be 135,000–140,000 and that of MDH to be 65,000–70,000. Employing electrophoretic mobility in starch gel, molecular hybridization, and immunoprecipitation, the LDH isozymes could be classified into three major groups designated a , b and c and a minor group designated β. The c group was found exclusively in the eye. All tissues examined contained a, b and c group isozymes. The interaction of skeletal muscle a ‐group isozymes with rabbit anti‐weak fish ( Cynoscion regalis ) A 4 LDH antiserum was weak and, therefore, similar to that of the group of isozymes of Prosobium cylindraceum previously designated f (Massaro, '72). Starch gel electrophoresis at pH 8.6 resolved three major isozymes of supernatant MDH. In skeletal muscle, two additional minor, weakly detectable isozymes were observed. These exhibited a greater net negative charge than the major isozymes and may be conformational isozymes. Article in Journal/Newspaper Thymallus arcticus Wiley Online Library Journal of Experimental Zoology 186 2 151 157 |
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Wiley Online Library |
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English |
description |
Abstract By gel exclusion chromatography the molecular weight of T. arcticus LDH was estimated to be 135,000–140,000 and that of MDH to be 65,000–70,000. Employing electrophoretic mobility in starch gel, molecular hybridization, and immunoprecipitation, the LDH isozymes could be classified into three major groups designated a , b and c and a minor group designated β. The c group was found exclusively in the eye. All tissues examined contained a, b and c group isozymes. The interaction of skeletal muscle a ‐group isozymes with rabbit anti‐weak fish ( Cynoscion regalis ) A 4 LDH antiserum was weak and, therefore, similar to that of the group of isozymes of Prosobium cylindraceum previously designated f (Massaro, '72). Starch gel electrophoresis at pH 8.6 resolved three major isozymes of supernatant MDH. In skeletal muscle, two additional minor, weakly detectable isozymes were observed. These exhibited a greater net negative charge than the major isozymes and may be conformational isozymes. |
format |
Article in Journal/Newspaper |
author |
Massaro, Edward J. |
spellingShingle |
Massaro, Edward J. Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas) |
author_facet |
Massaro, Edward J. |
author_sort |
Massaro, Edward J. |
title |
Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas) |
title_short |
Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas) |
title_full |
Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas) |
title_fullStr |
Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas) |
title_full_unstemmed |
Tissue distribution and properties of the lactate and supernatant Malate dehydrogenase isozymes of the grayling, Thymallus arcticus (Pallas) |
title_sort |
tissue distribution and properties of the lactate and supernatant malate dehydrogenase isozymes of the grayling, thymallus arcticus (pallas) |
publisher |
Wiley |
publishDate |
1973 |
url |
http://dx.doi.org/10.1002/jez.1401860206 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1401860206 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1401860206 |
genre |
Thymallus arcticus |
genre_facet |
Thymallus arcticus |
op_source |
Journal of Experimental Zoology volume 186, issue 2, page 151-157 ISSN 0022-104X 1097-010X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jez.1401860206 |
container_title |
Journal of Experimental Zoology |
container_volume |
186 |
container_issue |
2 |
container_start_page |
151 |
op_container_end_page |
157 |
_version_ |
1800739367998717952 |