Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides
Abstract Starch gel electrophoretic and molecular hybridization studies indicate that the genomes of rainbow ( Salmo gairdneri ) and brown ( S. trutta ) trouts may code for eight electrophoretically distinct types of lactate dehydrogenase (LDH) subunits. The interactions of these subunits in vivo pr...
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crwiley:10.1002/jez.1401680210 2024-06-02T08:03:41+00:00 Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides Massaro, Edward J. Markert, Clement L. 1968 http://dx.doi.org/10.1002/jez.1401680210 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1401680210 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1401680210 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Experimental Zoology volume 168, issue 2, page 223-238 ISSN 0022-104X 1097-010X journal-article 1968 crwiley https://doi.org/10.1002/jez.1401680210 2024-05-03T11:48:13Z Abstract Starch gel electrophoretic and molecular hybridization studies indicate that the genomes of rainbow ( Salmo gairdneri ) and brown ( S. trutta ) trouts may code for eight electrophoretically distinct types of lactate dehydrogenase (LDH) subunits. The interactions of these subunits in vivo produce eighteen isozymes. These isozymes are naturally arranged into four groups, designated a , b , d , and e , on the basis of physico‐chemical properties. Groups a , b , and d each consist of five isozymes while group e is composed of three isozymes. An obvious tissue specific distribution of the isozyme groups is observed. Like the rainbow and brown trouts, other salmonids such as the Atlantic salmon ( S. salar ), sockeye ( Onchorynchus nerka ), and bloater ( Coregonus hoyi ) possess numerous LDH isozymes. Also, we and others have detected an unusual multiplicity of molecular forms of other proteins in Salmonidae . Representatives of the Osmeridae , Clupeidae , Engraulidae , Argentidae , and Esocidae , which are closely related families, possess no more than five LDH isozymes. Moreover, salmonids contain twice as much DNA per cell and have karyotypes which show about twice as many chromosome arms as certain closely related forms. From these observations, it has been concluded that salmonids are tetraploids. In our view, it seems most likely that they arose through allotetraploidization of a primitive hybrid ancestor. Article in Journal/Newspaper Atlantic salmon Wiley Online Library Sockeye ENVELOPE(-130.143,-130.143,54.160,54.160) Journal of Experimental Zoology 168 2 223 238 |
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Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
Abstract Starch gel electrophoretic and molecular hybridization studies indicate that the genomes of rainbow ( Salmo gairdneri ) and brown ( S. trutta ) trouts may code for eight electrophoretically distinct types of lactate dehydrogenase (LDH) subunits. The interactions of these subunits in vivo produce eighteen isozymes. These isozymes are naturally arranged into four groups, designated a , b , d , and e , on the basis of physico‐chemical properties. Groups a , b , and d each consist of five isozymes while group e is composed of three isozymes. An obvious tissue specific distribution of the isozyme groups is observed. Like the rainbow and brown trouts, other salmonids such as the Atlantic salmon ( S. salar ), sockeye ( Onchorynchus nerka ), and bloater ( Coregonus hoyi ) possess numerous LDH isozymes. Also, we and others have detected an unusual multiplicity of molecular forms of other proteins in Salmonidae . Representatives of the Osmeridae , Clupeidae , Engraulidae , Argentidae , and Esocidae , which are closely related families, possess no more than five LDH isozymes. Moreover, salmonids contain twice as much DNA per cell and have karyotypes which show about twice as many chromosome arms as certain closely related forms. From these observations, it has been concluded that salmonids are tetraploids. In our view, it seems most likely that they arose through allotetraploidization of a primitive hybrid ancestor. |
format |
Article in Journal/Newspaper |
author |
Massaro, Edward J. Markert, Clement L. |
spellingShingle |
Massaro, Edward J. Markert, Clement L. Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides |
author_facet |
Massaro, Edward J. Markert, Clement L. |
author_sort |
Massaro, Edward J. |
title |
Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides |
title_short |
Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides |
title_full |
Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides |
title_fullStr |
Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides |
title_full_unstemmed |
Isozyme patterns of salmonid fishes: Evidence for multiple cistrons for lactate dehydrogenase polypeptides |
title_sort |
isozyme patterns of salmonid fishes: evidence for multiple cistrons for lactate dehydrogenase polypeptides |
publisher |
Wiley |
publishDate |
1968 |
url |
http://dx.doi.org/10.1002/jez.1401680210 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjez.1401680210 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jez.1401680210 |
long_lat |
ENVELOPE(-130.143,-130.143,54.160,54.160) |
geographic |
Sockeye |
geographic_facet |
Sockeye |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Experimental Zoology volume 168, issue 2, page 223-238 ISSN 0022-104X 1097-010X |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jez.1401680210 |
container_title |
Journal of Experimental Zoology |
container_volume |
168 |
container_issue |
2 |
container_start_page |
223 |
op_container_end_page |
238 |
_version_ |
1800748277844410368 |