Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts
Abstract BACKGROUND This study investigated the use of core–shell biocatalysts, synthesized using recombinant lipase B from Candida antarctica (CALB), for the production of isopropyl palmitate and the optimization of this biocatalytic route. RESULTS Initially, three distinct core–shell biocatalysts,...
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crwiley:10.1002/jctb.5782 2024-06-02T07:57:15+00:00 Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts Barsé, Laísa Q Graebin, Natália G Cipolatti, Eliane P Robert, Julia M Pinto, Martina CC Pinto, Jose CCS Freire, Denise MG Rodrigues, Rafael C Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Petrobras Conselho Nacional de Desenvolvimento Científico e Tecnológico 2018 http://dx.doi.org/10.1002/jctb.5782 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjctb.5782 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jctb.5782 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Chemical Technology & Biotechnology volume 94, issue 2, page 389-397 ISSN 0268-2575 1097-4660 journal-article 2018 crwiley https://doi.org/10.1002/jctb.5782 2024-05-03T11:44:40Z Abstract BACKGROUND This study investigated the use of core–shell biocatalysts, synthesized using recombinant lipase B from Candida antarctica (CALB), for the production of isopropyl palmitate and the optimization of this biocatalytic route. RESULTS Initially, three distinct core–shell biocatalysts, were evaluated for the production of isopropyl palmitate and the CALB‐P(S‐ co‐ DVB)/P(S‐ co ‐DVB) biocatalyst (biocatalyst made of polystyrene ‐co‐ divinylbenzene core–shell particles) was the most suitable biocatalyst for this purpose. The effect of organic solvents – n‐hexane, isooctane, n‐heptane and cyclohexane – in the ester conversion was evaluated, and the most suitable solvent was the isooctane. The optimal reaction conditions were pursued conducting an optimization statistical analysis. The most suitable reaction conditions were identified: reaction temperature, 55°C; substrate molar ratio, 1:1.42 (palmitic acid: isopropyl alcohol); biocatalyst content, 24% w/w. Finally, the reuse of the biocatalyst was evaluated and the relative enzymatic activity remained up to 50% after the fourth cycle. CONCLUSION Results were compared with commercial biocatalyst and the home‐made enzymatic biocatalyst (CALB‐P(S‐ co‐ DVB/PS‐ co ‐DVB) showed promise for application in the synthesis of isopropyl palmitate. The optimization study improved reaction conversion from 30% to 78%. The home‐made biocatalysts exhibited better performance for the synthesis of isopropyl palmitate compared with the commercial Novozym 435, using the same enzymatic activity. © 2018 Society of Chemical Industry Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Chemical Technology & Biotechnology 94 2 389 397 |
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Wiley Online Library |
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crwiley |
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English |
description |
Abstract BACKGROUND This study investigated the use of core–shell biocatalysts, synthesized using recombinant lipase B from Candida antarctica (CALB), for the production of isopropyl palmitate and the optimization of this biocatalytic route. RESULTS Initially, three distinct core–shell biocatalysts, were evaluated for the production of isopropyl palmitate and the CALB‐P(S‐ co‐ DVB)/P(S‐ co ‐DVB) biocatalyst (biocatalyst made of polystyrene ‐co‐ divinylbenzene core–shell particles) was the most suitable biocatalyst for this purpose. The effect of organic solvents – n‐hexane, isooctane, n‐heptane and cyclohexane – in the ester conversion was evaluated, and the most suitable solvent was the isooctane. The optimal reaction conditions were pursued conducting an optimization statistical analysis. The most suitable reaction conditions were identified: reaction temperature, 55°C; substrate molar ratio, 1:1.42 (palmitic acid: isopropyl alcohol); biocatalyst content, 24% w/w. Finally, the reuse of the biocatalyst was evaluated and the relative enzymatic activity remained up to 50% after the fourth cycle. CONCLUSION Results were compared with commercial biocatalyst and the home‐made enzymatic biocatalyst (CALB‐P(S‐ co‐ DVB/PS‐ co ‐DVB) showed promise for application in the synthesis of isopropyl palmitate. The optimization study improved reaction conversion from 30% to 78%. The home‐made biocatalysts exhibited better performance for the synthesis of isopropyl palmitate compared with the commercial Novozym 435, using the same enzymatic activity. © 2018 Society of Chemical Industry |
author2 |
Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Petrobras Conselho Nacional de Desenvolvimento Científico e Tecnológico |
format |
Article in Journal/Newspaper |
author |
Barsé, Laísa Q Graebin, Natália G Cipolatti, Eliane P Robert, Julia M Pinto, Martina CC Pinto, Jose CCS Freire, Denise MG Rodrigues, Rafael C |
spellingShingle |
Barsé, Laísa Q Graebin, Natália G Cipolatti, Eliane P Robert, Julia M Pinto, Martina CC Pinto, Jose CCS Freire, Denise MG Rodrigues, Rafael C Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts |
author_facet |
Barsé, Laísa Q Graebin, Natália G Cipolatti, Eliane P Robert, Julia M Pinto, Martina CC Pinto, Jose CCS Freire, Denise MG Rodrigues, Rafael C |
author_sort |
Barsé, Laísa Q |
title |
Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts |
title_short |
Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts |
title_full |
Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts |
title_fullStr |
Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts |
title_full_unstemmed |
Production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts |
title_sort |
production and optimization of isopropyl palmitate via biocatalytic route using home‐made enzymatic catalysts |
publisher |
Wiley |
publishDate |
2018 |
url |
http://dx.doi.org/10.1002/jctb.5782 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjctb.5782 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jctb.5782 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Chemical Technology & Biotechnology volume 94, issue 2, page 389-397 ISSN 0268-2575 1097-4660 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jctb.5782 |
container_title |
Journal of Chemical Technology & Biotechnology |
container_volume |
94 |
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2 |
container_start_page |
389 |
op_container_end_page |
397 |
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1800740194831302656 |