Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates
Abstract Background Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates ( PHA ) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial . Results The effects of reaction variables reaction t...
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crwiley:10.1002/jctb.3980 2024-06-02T07:58:18+00:00 Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates Gumel, Ahmad M Annuar, Suffian M Heidelberg, Thorsten 2012 http://dx.doi.org/10.1002/jctb.3980 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjctb.3980 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jctb.3980 en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Journal of Chemical Technology & Biotechnology volume 88, issue 7, page 1328-1335 ISSN 0268-2575 1097-4660 journal-article 2012 crwiley https://doi.org/10.1002/jctb.3980 2024-05-03T11:23:41Z Abstract Background Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates ( PHA ) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial . Results The effects of reaction variables reaction time, temperature, enzyme concentration and substrate ratio on reaction rate and yield in the synthesis of poly(1'‐ O ‐3‐hydroxyacyl‐sucrose) using Candida antarctica lipase B ( EC 3.1.1.3) were studied. Using H 2 O 2 as micro‐initiator, enzyme‐mediated synthesis yielded reaction rate, v app of 0.076 x 10 −5 mol L −1 s −1 . The biodegradability of the functionalized polymer was observed to increase by 1.5 fold compared with the non‐functionalized material apart from showing better compostability. Increasing the reaction temperature (>50°C), enzyme concentration (>15 g L −1 ) and reactant ratio (w/w) of sucrose: PHA (>2) did not increase further the rate or yield. The sucrose‐functionalized mcl‐ PHA was characterized with respect to the non‐functionalized material . Conclusions Novozym® 435 can be used effectively to synthesize poly(1'‐ O ‐3‐hydroxyacyl sucrose) in micro‐aqueous medium bypassing the need for chemo‐synthetic steps. The synthesized biomaterials have potential applications in biomedical and industrial niches.© 2012 Society of Chemical Industry Article in Journal/Newspaper Antarc* Antarctica Wiley Online Library Journal of Chemical Technology & Biotechnology 88 7 1328 1335 |
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English |
description |
Abstract Background Functionalization of aliphatic biopolymers such as bacterial polyhydroxyalkanoates ( PHA ) using biologically active hydrophilic moieties like sugars helps to improve the hydrophilicity and biodegradability of the biomaterial . Results The effects of reaction variables reaction time, temperature, enzyme concentration and substrate ratio on reaction rate and yield in the synthesis of poly(1'‐ O ‐3‐hydroxyacyl‐sucrose) using Candida antarctica lipase B ( EC 3.1.1.3) were studied. Using H 2 O 2 as micro‐initiator, enzyme‐mediated synthesis yielded reaction rate, v app of 0.076 x 10 −5 mol L −1 s −1 . The biodegradability of the functionalized polymer was observed to increase by 1.5 fold compared with the non‐functionalized material apart from showing better compostability. Increasing the reaction temperature (>50°C), enzyme concentration (>15 g L −1 ) and reactant ratio (w/w) of sucrose: PHA (>2) did not increase further the rate or yield. The sucrose‐functionalized mcl‐ PHA was characterized with respect to the non‐functionalized material . Conclusions Novozym® 435 can be used effectively to synthesize poly(1'‐ O ‐3‐hydroxyacyl sucrose) in micro‐aqueous medium bypassing the need for chemo‐synthetic steps. The synthesized biomaterials have potential applications in biomedical and industrial niches.© 2012 Society of Chemical Industry |
format |
Article in Journal/Newspaper |
author |
Gumel, Ahmad M Annuar, Suffian M Heidelberg, Thorsten |
spellingShingle |
Gumel, Ahmad M Annuar, Suffian M Heidelberg, Thorsten Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates |
author_facet |
Gumel, Ahmad M Annuar, Suffian M Heidelberg, Thorsten |
author_sort |
Gumel, Ahmad M |
title |
Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates |
title_short |
Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates |
title_full |
Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates |
title_fullStr |
Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates |
title_full_unstemmed |
Single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates |
title_sort |
single‐step lipase‐catalyzed functionalization of medium‐chain‐length polyhydroxyalkanoates |
publisher |
Wiley |
publishDate |
2012 |
url |
http://dx.doi.org/10.1002/jctb.3980 https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjctb.3980 https://onlinelibrary.wiley.com/doi/pdf/10.1002/jctb.3980 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Journal of Chemical Technology & Biotechnology volume 88, issue 7, page 1328-1335 ISSN 0268-2575 1097-4660 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1002/jctb.3980 |
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Journal of Chemical Technology & Biotechnology |
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88 |
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7 |
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1328 |
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1335 |
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1800741596697722880 |