Response surface methodology as a tool to study the lipase‐catalyzed synthesis of betulinic acid ester

• Published in: Journal of Chemical Technology & Biotechnology
• Main Authors: Yasin, Yamin, Basri, Mahiran, Ahmad, Faujan, Salleh, Abu Bakar
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2008
• Subjects: Antarc*; Antarctica
• Online Access: http://dx.doi.org/10.1002/jctb.1858; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjctb.1858; https://onlinelibrary.wiley.com/doi/pdf/10.1002/jctb.1858

Abstract BACKGROUND: The synthesis of betulinic acid ester using betulinic acid and oleyl alcohol catalyzed by Novozym 435 (immobilized Candida antarctica lipase) was carried out. Response surface methodology (RSM) based on a five‐level, three‐variable, central composite rotatable design (CCRD) was employed to evaluate the interactive effects of various parameters. The parameters were reaction time (8–16 h), temperature (20–60 °C) and enzyme amount (120–160 mg). RESULTS: Simultaneously increasing reaction time, temperature and amount of enzyme increased the yields of betulinic acid ester produced. CONCLUSION: The optimum conditions derived via RSM for the reaction were reaction time of 10.2 h, temperature of 53.1 °C and enzyme amount of 138 mg. The actual experimental yield was 48.5% under optimum conditions, which compared well with the maximum predicted value of 47.6%. Copyright © 2008 Society of Chemical Industry